Restriction map for
pF1KB0409
(Linear) (Six-base) MAPSORT of: KIBB0409.seq Check: 2805 from: 1 to: 1398
KIBB0409.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
Acc65I G'GTAC_C
Cuts at: 0 1247 1398
Size: 1247 151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 1398
Size: 28 1370
AfeI AGC'GCT
Cuts at: 0 508 1398
Size: 508 890
AflIII A'CryG_T
Cuts at: 0 185 1157 1398
Size: 185 972 241
Fragments arranged by size:
972 241 185
AhdI GACnn_n'nnGTC
Cuts at: 0 552 1398
Size: 552 846
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1144 1176 1398
Size: 1144 32 222
Fragments arranged by size:
1144 222 32
AlwNI CAG_nnn'CTG
Cuts at: 0 684 1028 1041 1398
Size: 684 344 13 357
Fragments arranged by size:
684 357 344 13
AscI GG'CGCG_CC
Cuts at: 0 177 1398
Size: 177 1221
AvaI C'yCGr_G
Cuts at: 0 260 885 1398
Size: 260 625 513
Fragments arranged by size:
625 513 260
BanI G'GyrC_C
Cuts at: 0 170 499 518 889 954 1051 1247 1398
Size: 170 329 19 371 65 97 196 151
Fragments arranged by size:
371 329 196 170 151 97 65 19
BanII G_rGCy'C
Cuts at: 0 214 358 705 1070 1389 1398
Size: 214 144 347 365 319 9
Fragments arranged by size:
365 347 319 214 144 9
BbeI G_GCGC'C
Cuts at: 0 174 1055 1398
Size: 174 881 343
Fragments arranged by size:
881 343 174
BbsI GAAGACnn'nnnn_
Cuts at: 0 123 1398
Size: 123 1275
BbvCI CC'TCA_GC
Cuts at: 0 817 1398
Size: 817 581
BclI T'GATC_A
Cuts at: 0 725 1398
Size: 725 673
BglII A'GATC_T
Cuts at: 0 266 1398
Size: 266 1132
Bme1580I G_kGCm'C
Cuts at: 0 502 658 892 959 1088 1243 1398
Size: 502 156 234 67 129 155 155
Fragments arranged by size:
502 234 156 155 155 129 67
BmrI ACTGGGnnnn_n'
Cuts at: 0 1317 1398
Size: 1317 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 261 549 1398
Size: 261 288 849
Fragments arranged by size:
849 288 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 238 358 801 1084 1398
Size: 214 24 120 443 283 314
Fragments arranged by size:
443 314 283 214 120 24
Bpu10I CC'TnA_GC
Cuts at: 0 74 817 1398
Size: 74 743 581
Fragments arranged by size:
743 581 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 1398
Size: 318 1080
BsaI GGTCTCn'nnnn_
Cuts at: 0 9 750 1323 1398
Size: 9 741 573 75
Fragments arranged by size:
741 573 75 9
BsaAI yAC'GTr
Cuts at: 0 878 1244 1398
Size: 878 366 154
Fragments arranged by size:
878 366 154
BsaHI Gr'CG_yC
Cuts at: 0 158 171 1052 1398
Size: 158 13 881 346
Fragments arranged by size:
881 346 158 13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 211 226 241 256 1144 1174 1398
Size: 211 15 15 15 888 30 224
Fragments arranged by size:
888 224 211 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 13 101 211 250 268 1398
Size: 13 88 110 39 18 1130
Fragments arranged by size:
1130 110 88 39 18 13
BseYI C'CCAG_C
Cuts at: 0 801 871 1028 1268 1398
Size: 801 70 157 240 130
Fragments arranged by size:
801 240 157 130 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 543 907 1398
Size: 148 395 364 491
Fragments arranged by size:
491 395 364 148
BsiEI CG_ry'CG
Cuts at: 0 208 1398
Size: 208 1190
BsiHKAI G_wGCw'C
Cuts at: 0 247 705 812 827 1070 1398
Size: 247 458 107 15 243 328
Fragments arranged by size:
458 328 247 243 107 15
BsiWI C'GTAC_G
Cuts at: 0 878 1398
Size: 878 520
BsmBI CGTCTCn'nnnn_
Cuts at: 0 418 1398
Size: 418 980
Bsp1286I G_dGCh'C
Cuts at: 0 214 247 358 502 658 705 812 827
Size: 214 33 111 144 156 47 107 15
Cuts at: 827 892 959 1070 1088 1243 1389 1398
Size: 65 67 111 18 155 146 9
Fragments arranged by size:
214 156 155 146 144 111 111 107
67 65 47 33 18 15 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 669 849 1398
Size: 669 180 549
Fragments arranged by size:
669 549 180
BsrFI r'CCGG_y
Cuts at: 0 167 173 845 1168 1398
Size: 167 6 672 323 230
Fragments arranged by size:
672 323 230 167 6
BsrGI T'GTAC_A
Cuts at: 0 1307 1398
Size: 1307 91
BssHII G'CGCG_C
Cuts at: 0 177 1398
Size: 177 1221
BssSI C'ACGA_G
Cuts at: 0 523 1398
Size: 523 875
BstAPI GCAn_nnn'nTGC
Cuts at: 0 653 1398
Size: 653 745
BstXI CCAn_nnnn'nTGG
Cuts at: 0 741 1062 1398
Size: 741 321 336
Fragments arranged by size:
741 336 321
BstYI r'GATC_y
Cuts at: 0 266 1398
Size: 266 1132
Bsu36I CC'TnA_GG
Cuts at: 0 16 81 1398
Size: 16 65 1317
Fragments arranged by size:
1317 65 16
BtgI C'CryG_G
Cuts at: 0 106 153 673 749 932 1047 1055 1289
Size: 106 47 520 76 183 115 8 234
Cuts at: 1289 1398
Size: 109
Fragments arranged by size:
520 234 183 115 109 106 76 47
8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 891 1398
Size: 891 507
BtsI GCAGTG_nn'
Cuts at: 0 247 492 1398
Size: 247 245 906
Fragments arranged by size:
906 247 245
EaeI y'GGCC_r
Cuts at: 0 205 1398
Size: 205 1193
EagI C'GGCC_G
Cuts at: 0 205 1398
Size: 205 1193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 907 1398
Size: 907 491
EcoICRI GAG'CTC
Cuts at: 0 703 1068 1398
Size: 703 365 330
Fragments arranged by size:
703 365 330
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 214 238 358 801 1084 1398
Size: 28 186 24 120 443 283 314
Fragments arranged by size:
443 314 283 186 120 28 24
EcoO109I rG'GnC_Cy
Cuts at: 0 12 118 681 719 1398
Size: 12 106 563 38 679
Fragments arranged by size:
679 563 106 38 12
FspI TGC'GCA
Cuts at: 0 1284 1398
Size: 1284 114
HaeII r_GCGC'y
Cuts at: 0 174 510 1055 1398
Size: 174 336 545 343
Fragments arranged by size:
545 343 336 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 538 570 1398
Size: 538 32 828
Fragments arranged by size:
828 538 32
KasI G'GCGC_C
Cuts at: 0 170 1051 1398
Size: 170 881 347
Fragments arranged by size:
881 347 170
KpnI G_GTAC'C
Cuts at: 0 1251 1398
Size: 1251 147
MluI A'CGCG_T
Cuts at: 0 185 1398
Size: 185 1213
MslI CAynn'nnrTG
Cuts at: 0 434 739 1398
Size: 434 305 659
Fragments arranged by size:
659 434 305
MspA1I CmG'CkG
Cuts at: 0 444 751 1025 1038 1268 1398
Size: 444 307 274 13 230 130
Fragments arranged by size:
444 307 274 230 130 13
NaeI GCC'GGC
Cuts at: 0 175 847 1170 1398
Size: 175 672 323 228
Fragments arranged by size:
672 323 228 175
NarI GG'CG_CC
Cuts at: 0 171 1052 1398
Size: 171 881 346
Fragments arranged by size:
881 346 171
NcoI C'CATG_G
Cuts at: 0 673 1398
Size: 673 725
NgoMIV G'CCGG_C
Cuts at: 0 173 845 1168 1398
Size: 173 672 323 230
Fragments arranged by size:
672 323 230 173
NspI r_CATG'y
Cuts at: 0 105 1161 1225 1398
Size: 105 1056 64 173
Fragments arranged by size:
1056 173 105 64
PciI A'CATG_T
Cuts at: 0 1157 1398
Size: 1157 241
PflMI CCAn_nnn'nTGG
Cuts at: 0 933 1041 1398
Size: 933 108 357
Fragments arranged by size:
933 357 108
PfoI T'CCnGG_A
Cuts at: 0 191 1398
Size: 191 1207
PmlI CAC'GTG
Cuts at: 0 1244 1398
Size: 1244 154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1144 1176 1398
Size: 1144 32 222
Fragments arranged by size:
1144 222 32
PpuMI rG'GwC_Cy
Cuts at: 0 681 719 1398
Size: 681 38 679
Fragments arranged by size:
681 679 38
PstI C_TGCA'G
Cuts at: 0 63 487 1115 1398
Size: 63 424 628 283
Fragments arranged by size:
628 424 283 63
PvuII CAG'CTG
Cuts at: 0 444 1038 1268 1398
Size: 444 594 230 130
Fragments arranged by size:
594 444 230 130
SacI G_AGCT'C
Cuts at: 0 705 1070 1398
Size: 705 365 328
Fragments arranged by size:
705 365 328
SacII CC_GC'GG
Cuts at: 0 752 1398
Size: 752 646
SanDI GG'GwC_CC
Cuts at: 0 719 1398
Size: 719 679
ScaI AGT'ACT
Cuts at: 0 481 1398
Size: 481 917
SfcI C'TryA_G
Cuts at: 0 59 483 1111 1398
Size: 59 424 628 287
Fragments arranged by size:
628 424 287 59
SfoI GGC'GCC
Cuts at: 0 172 1053 1398
Size: 172 881 345
Fragments arranged by size:
881 345 172
SgrAI Cr'CCGG_yG
Cuts at: 0 167 173 1398
Size: 167 6 1225
Fragments arranged by size:
1225 167 6
SmaI CCC'GGG
Cuts at: 0 262 887 1398
Size: 262 625 511
Fragments arranged by size:
625 511 262
SmlI C'TyrA_G
Cuts at: 0 333 1398
Size: 333 1065
StuI AGG'CCT
Cuts at: 0 79 1398
Size: 79 1319
StyI C'CwwG_G
Cuts at: 0 627 673 1398
Size: 627 46 725
Fragments arranged by size:
725 627 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 579 729 1243 1398
Size: 579 150 514 155
Fragments arranged by size:
579 514 155 150
TatI w'GTAC_w
Cuts at: 0 479 1154 1307 1325 1398
Size: 479 675 153 18 73
Fragments arranged by size:
675 479 153 73 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 91 123 211 226 243 258 1398
Size: 91 32 88 15 17 15 1140
Fragments arranged by size:
1140 91 88 32 17 15 15
XbaI T'CTAG_A
Cuts at: 0 600 1398
Size: 600 798
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 189 1398
Size: 189 1209
XmaI C'CCGG_G
Cuts at: 0 260 885 1398
Size: 260 625 513
Fragments arranged by size:
625 513 260
XmnI GAAnn'nnTTC
Cuts at: 0 295 1398
Size: 295 1103
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AhdI AloI AlwNI AscI
AvaI BanI BanII BbeI BbsI BbvCI BclI BglII
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspMI BsrFI BsrGI BssHII BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EagI
EciI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I KasI
KpnI MluI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmlI PpiI PpuMI PstI
PvuII SacI SacII SanDI ScaI SfcI SfoI SgrAI
SmaI SmlI StuI StyI TaqII TatI TstI XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AleI AlfI
ApaI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI
BcgI BciVI BfrBI BglI BlpI BmgBI BmtI BsaBI
BsaWI BsmI BspEI BspHI BsrBI BsrDI BstBI BstEII
BstZ17I ClaI CspCI DraI DraIII DrdI EarI EcoNI
EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI
MfeI MmeI MscI NdeI NheI NotI NruI NsiI
PacI PasI PmeI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SalI SapI SbfI SexAI SfiI SgfI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XhoI
ZraI