Restriction map for
pF1KB0410
(Linear) (Six-base) MAPSORT of: KIBB0410.seq Check: 5243 from: 1 to: 2466
KIBB0410.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 524 2466
Size: 524 1942
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1568 1760 2466
Size: 1568 192 706
Fragments arranged by size:
1568 706 192
AfeI AGC'GCT
Cuts at: 0 132 2466
Size: 132 2334
AleI CACnn'nnGTG
Cuts at: 0 322 2466
Size: 322 2144
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 277 340 2466
Size: 277 63 2126
Fragments arranged by size:
2126 277 63
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 433 465 2466
Size: 433 32 2001
Fragments arranged by size:
2001 433 32
AlwNI CAG_nnn'CTG
Cuts at: 0 303 1575 2466
Size: 303 1272 891
Fragments arranged by size:
1272 891 303
ApaI G_GGCC'C
Cuts at: 0 32 128 2466
Size: 32 96 2338
Fragments arranged by size:
2338 96 32
ApaLI G'TGCA_C
Cuts at: 0 283 1111 2466
Size: 283 828 1355
Fragments arranged by size:
1355 828 283
ApoI r'AATT_y
Cuts at: 0 394 1022 2466
Size: 394 628 1444
Fragments arranged by size:
1444 628 394
AvaI C'yCGr_G
Cuts at: 0 20 120 291 697 2466
Size: 20 100 171 406 1769
Fragments arranged by size:
1769 406 171 100 20
BanI G'GyrC_C
Cuts at: 0 324 536 2466
Size: 324 212 1930
Fragments arranged by size:
1930 324 212
BanII G_rGCy'C
Cuts at: 0 32 128 272 1450 1943 2466
Size: 32 96 144 1178 493 523
Fragments arranged by size:
1178 523 493 144 96 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 1198 2454 2466
Size: 1198 1256 12
Fragments arranged by size:
1256 1198 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 28 62 955 989 2466
Size: 28 34 893 34 1477
Fragments arranged by size:
1477 893 34 34 28
BclI T'GATC_A
Cuts at: 0 1490 2466
Size: 1490 976
BglI GCCn_nnn'nGGC
Cuts at: 0 16 2466
Size: 16 2450
BglII A'GATC_T
Cuts at: 0 629 2255 2466
Size: 629 1626 211
Fragments arranged by size:
1626 629 211
BlpI GC'TnA_GC
Cuts at: 0 1568 2466
Size: 1568 898
Bme1580I G_kGCm'C
Cuts at: 0 32 128 287 329 1115 2466
Size: 32 96 159 42 786 1351
Fragments arranged by size:
1351 786 159 96 42 32
BmgBI CAC'GTC
Cuts at: 0 282 2466
Size: 282 2184
BmrI ACTGGGnnnn_n'
Cuts at: 0 736 932 2466
Size: 736 196 1534
Fragments arranged by size:
1534 736 196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 1308 1988 1997 2381 2466
Size: 286 1022 680 9 384 85
Fragments arranged by size:
1022 680 384 286 85 9
Bpu10I CC'TnA_GC
Cuts at: 0 2231 2466
Size: 2231 235
BsaI GGTCTCn'nnnn_
Cuts at: 0 512 646 2466
Size: 512 134 1820
Fragments arranged by size:
1820 512 134
BsaWI w'CCGG_w
Cuts at: 0 2312 2466
Size: 2312 154
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 79 340 735 1929 1951 2466
Size: 76 3 261 395 1194 22 515
Fragments arranged by size:
1194 515 395 261 76 22 3
BseYI C'CCAG_C
Cuts at: 0 127 776 2466
Size: 127 649 1690
Fragments arranged by size:
1690 649 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2074 2162 2466
Size: 2074 88 304
Fragments arranged by size:
2074 304 88
BsiEI CG_ry'CG
Cuts at: 0 11 170 2466
Size: 11 159 2296
Fragments arranged by size:
2296 159 11
BsiHKAI G_wGCw'C
Cuts at: 0 272 287 824 968 1115 2237 2466
Size: 272 15 537 144 147 1122 229
Fragments arranged by size:
1122 537 272 229 147 144 15
BsmI GAATG_Cn'
Cuts at: 0 136 805 2466
Size: 136 669 1661
Fragments arranged by size:
1661 669 136
Bsp1286I G_dGCh'C
Cuts at: 0 32 128 272 287 329 824 968 1115
Size: 32 96 144 15 42 495 144 147
Cuts at: 1115 1450 1943 2237 2466
Size: 335 493 294 229
Fragments arranged by size:
495 493 335 294 229 147 144 144
96 42 32 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 524 771 1596 2466
Size: 524 247 825 870
Fragments arranged by size:
870 825 524 247
BsrBI CCG'CTC
Cuts at: 0 194 336 2466
Size: 194 142 2130
Fragments arranged by size:
2130 194 142
BsrDI GCAATG_nn'
Cuts at: 0 1851 2378 2466
Size: 1851 527 88
Fragments arranged by size:
1851 527 88
BsrFI r'CCGG_y
Cuts at: 0 165 2466
Size: 165 2301
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1905 2466
Size: 1905 561
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1890 2466
Size: 1890 576
BstYI r'GATC_y
Cuts at: 0 629 2255 2403 2466
Size: 629 1626 148 63
Fragments arranged by size:
1626 629 148 63
Bsu36I CC'TnA_GG
Cuts at: 0 458 1741 2466
Size: 458 1283 725
Fragments arranged by size:
1283 725 458
BtgI C'CryG_G
Cuts at: 0 32 242 416 512 557 2127 2466
Size: 32 210 174 96 45 1570 339
Fragments arranged by size:
1570 339 210 174 96 45 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 240 536 2466
Size: 240 296 1930
Fragments arranged by size:
1930 296 240
BtsI GCAGTG_nn'
Cuts at: 0 607 822 1267 1448 1707 2466
Size: 607 215 445 181 259 759
Fragments arranged by size:
759 607 445 259 215 181
DraI TTT'AAA
Cuts at: 0 1643 2466
Size: 1643 823
DraIII CAC_nnn'GTG
Cuts at: 0 231 2466
Size: 231 2235
DrdI GACnn_nn'nnGTC
Cuts at: 0 418 2466
Size: 418 2048
EaeI y'GGCC_r
Cuts at: 0 8 167 1466 2298 2466
Size: 8 159 1299 832 168
Fragments arranged by size:
1299 832 168 159 8
EagI C'GGCC_G
Cuts at: 0 8 167 2466
Size: 8 159 2299
Fragments arranged by size:
2299 159 8
EarI CTCTTCn'nnn_
Cuts at: 0 906 1991 2153 2466
Size: 906 1085 162 313
Fragments arranged by size:
1085 906 313 162
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 130 2466
Size: 130 2336
EcoICRI GAG'CTC
Cuts at: 0 270 2466
Size: 270 2196
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 1308 1568 1760 1988 1997 2381 2466
Size: 286 1022 260 192 228 9 384 85
Fragments arranged by size:
1022 384 286 260 228 192 85 9
EcoO109I rG'GnC_Cy
Cuts at: 0 28 124 300 411 681 2466
Size: 28 96 176 111 270 1785
Fragments arranged by size:
1785 270 176 111 96 28
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1205 2088 2466
Size: 1205 883 378
Fragments arranged by size:
1205 883 378
HaeII r_GCGC'y
Cuts at: 0 134 2466
Size: 134 2332
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1737 1769 2466
Size: 1737 32 697
Fragments arranged by size:
1737 697 32
HincII GTy'rAC
Cuts at: 0 618 1512 2466
Size: 618 894 954
Fragments arranged by size:
954 894 618
HindIII A'AGCT_T
Cuts at: 0 1676 1729 2466
Size: 1676 53 737
Fragments arranged by size:
1676 737 53
HpaI GTT'AAC
Cuts at: 0 1512 2466
Size: 1512 954
MfeI C'AATT_G
Cuts at: 0 914 1274 2466
Size: 914 360 1192
Fragments arranged by size:
1192 914 360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 2466
Size: 419 2047
MscI TGG'CCA
Cuts at: 0 1468 2300 2466
Size: 1468 832 166
Fragments arranged by size:
1468 832 166
MslI CAynn'nnrTG
Cuts at: 0 259 322 2466
Size: 259 63 2144
Fragments arranged by size:
2144 259 63
MspA1I CmG'CkG
Cuts at: 0 34 109 207 344 503 612 873 1347
Size: 34 75 98 137 159 109 261 474
Cuts at: 1347 1564 2129 2466
Size: 217 565 337
Fragments arranged by size:
565 474 337 261 217 159 137 109
98 75 34
NaeI GCC'GGC
Cuts at: 0 167 2466
Size: 167 2299
NcoI C'CATG_G
Cuts at: 0 512 557 2466
Size: 512 45 1909
Fragments arranged by size:
1909 512 45
NgoMIV G'CCGG_C
Cuts at: 0 165 2466
Size: 165 2301
NspI r_CATG'y
Cuts at: 0 735 2466
Size: 735 1731
PflMI CCAn_nnn'nTGG
Cuts at: 0 303 2466
Size: 303 2163
PfoI T'CCnGG_A
Cuts at: 0 1574 2006 2466
Size: 1574 432 460
Fragments arranged by size:
1574 460 432
PpuMI rG'GwC_Cy
Cuts at: 0 300 411 2466
Size: 300 111 2055
Fragments arranged by size:
2055 300 111
PshAI GACnn'nnGTC
Cuts at: 0 742 2466
Size: 742 1724
PspOMI G'GGCC_C
Cuts at: 0 28 124 2466
Size: 28 96 2342
Fragments arranged by size:
2342 96 28
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1299 1331 2466
Size: 1299 32 1135
Fragments arranged by size:
1299 1135 32
PstI C_TGCA'G
Cuts at: 0 182 602 1293 1910 2466
Size: 182 420 691 617 556
Fragments arranged by size:
691 617 556 420 182
PvuII CAG'CTG
Cuts at: 0 344 503 612 873 1347 1564 2466
Size: 344 159 109 261 474 217 902
Fragments arranged by size:
902 474 344 261 217 159 109
SacI G_AGCT'C
Cuts at: 0 272 2466
Size: 272 2194
SacII CC_GC'GG
Cuts at: 0 35 2130 2466
Size: 35 2095 336
Fragments arranged by size:
2095 336 35
SbfI CC_TGCA'GG
Cuts at: 0 1910 2466
Size: 1910 556
SexAI A'CCwGG_T
Cuts at: 0 302 1523 2466
Size: 302 1221 943
Fragments arranged by size:
1221 943 302
SfcI C'TryA_G
Cuts at: 0 178 598 1289 1906 2238 2466
Size: 178 420 691 617 332 228
Fragments arranged by size:
691 617 420 332 228 178
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 16 2466
Size: 16 2450
SmaI CCC'GGG
Cuts at: 0 22 122 293 2466
Size: 22 100 171 2173
Fragments arranged by size:
2173 171 100 22
SpeI A'CTAG_T
Cuts at: 0 1821 2466
Size: 1821 645
SphI G_CATG'C
Cuts at: 0 735 2466
Size: 735 1731
StuI AGG'CCT
Cuts at: 0 1259 1904 2466
Size: 1259 645 562
Fragments arranged by size:
1259 645 562
StyI C'CwwG_G
Cuts at: 0 407 512 557 2016 2466
Size: 407 105 45 1459 450
Fragments arranged by size:
1459 450 407 105 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 790 1813 2466
Size: 790 1023 653
Fragments arranged by size:
1023 790 653
TatI w'GTAC_w
Cuts at: 0 494 2466
Size: 494 1972
XmaI C'CCGG_G
Cuts at: 0 20 120 291 2466
Size: 20 100 171 2175
Fragments arranged by size:
2175 171 100 20
Enzymes that do cut:
AarI AcuI AfeI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbsI BcgI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BsaI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII
Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PflMI PfoI PpuMI
PshAI PspOMI PsrI PstI PvuII SacI SacII SbfI
SexAI SfcI SfiI SmaI SpeI SphI StuI StyI
TaqII TatI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI
BciVI BfrBI BmtI BplI BpuEI BsaAI BsaBI BsaHI
BsaXI BsiWI BsmBI BspEI BspHI BsrGI BssHII BssSI
BstBI BstEII BstZ17I ClaI CspCI EcoNI EcoRI EcoRV
FseI FspI FspAI KasI KpnI MluI NarI NdeI
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PsiI PspXI PvuI RsrII SalI SanDI
SapI ScaI SfoI SgfI SgrAI SmlI SnaBI SrfI
SspI SwaI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI