Restriction map for
pF1KB0411
(Linear) (Six-base) MAPSORT of: KIBB0411.seq Check: 5169 from: 1 to: 1401
KIBB0411.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AccI GT'mk_AC
Cuts at: 0 455 1401
Size: 455 946
Acc65I G'GTAC_C
Cuts at: 0 207 1401
Size: 207 1194
AfeI AGC'GCT
Cuts at: 0 988 1401
Size: 988 413
AhdI GACnn_n'nnGTC
Cuts at: 0 146 1401
Size: 146 1255
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1083 1246 1401
Size: 1083 163 155
Fragments arranged by size:
1083 163 155
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 402 434 626 658 1401
Size: 402 32 192 32 743
Fragments arranged by size:
743 402 192 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1200 1401
Size: 1200 201
ApaLI G'TGCA_C
Cuts at: 0 1281 1401
Size: 1281 120
ApoI r'AATT_y
Cuts at: 0 320 679 1401
Size: 320 359 722
Fragments arranged by size:
722 359 320
AseI AT'TA_AT
Cuts at: 0 49 1401
Size: 49 1352
AvaI C'yCGr_G
Cuts at: 0 434 1401
Size: 434 967
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 199 232 1401
Size: 199 33 1169
Fragments arranged by size:
1169 199 33
BanI G'GyrC_C
Cuts at: 0 207 439 967 1401
Size: 207 232 528 434
Fragments arranged by size:
528 434 232 207
BanII G_rGCy'C
Cuts at: 0 886 1401
Size: 886 515
BbeI G_GCGC'C
Cuts at: 0 443 1401
Size: 443 958
BbsI GAAGACnn'nnnn_
Cuts at: 0 1058 1401
Size: 1058 343
BlpI GC'TnA_GC
Cuts at: 0 999 1401
Size: 999 402
Bme1580I G_kGCm'C
Cuts at: 0 970 1285 1401
Size: 970 315 116
Fragments arranged by size:
970 315 116
BmrI ACTGGGnnnn_n'
Cuts at: 0 125 502 1401
Size: 125 377 899
Fragments arranged by size:
899 377 125
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 676 1126 1401
Size: 667 9 450 275
Fragments arranged by size:
667 450 275 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 1297 1401
Size: 697 600 104
Fragments arranged by size:
697 600 104
BsaHI Gr'CG_yC
Cuts at: 0 440 1401
Size: 440 961
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 626 656 1401
Size: 626 30 745
Fragments arranged by size:
745 626 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 921 1401
Size: 921 480
BsiHKAI G_wGCw'C
Cuts at: 0 16 1285 1401
Size: 16 1269 116
Fragments arranged by size:
1269 116 16
Bsp1286I G_dGCh'C
Cuts at: 0 16 886 970 1285 1401
Size: 16 870 84 315 116
Fragments arranged by size:
870 315 116 84 16
BspHI T'CATG_A
Cuts at: 0 753 1401
Size: 753 648
BsrDI GCAATG_nn'
Cuts at: 0 1055 1265 1401
Size: 1055 210 136
Fragments arranged by size:
1055 210 136
BsrGI T'GTAC_A
Cuts at: 0 1019 1401
Size: 1019 382
BssSI C'ACGA_G
Cuts at: 0 559 1401
Size: 559 842
BstZ17I GTA'TAC
Cuts at: 0 456 1401
Size: 456 945
Bsu36I CC'TnA_GG
Cuts at: 0 851 1401
Size: 851 550
BtgI C'CryG_G
Cuts at: 0 211 1401
Size: 211 1190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 552 1401
Size: 552 849
BtsI GCAGTG_nn'
Cuts at: 0 278 1401
Size: 278 1123
DraI TTT'AAA
Cuts at: 0 62 1401
Size: 62 1339
EaeI y'GGCC_r
Cuts at: 0 198 1401
Size: 198 1203
EarI CTCTTCn'nnn_
Cuts at: 0 29 104 1401
Size: 29 75 1297
Fragments arranged by size:
1297 75 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 676 1126 1401
Size: 667 9 450 275
Fragments arranged by size:
667 450 275 9
EcoO109I rG'GnC_Cy
Cuts at: 0 855 1158 1401
Size: 855 303 243
Fragments arranged by size:
855 303 243
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 474 1401
Size: 474 927
HaeII r_GCGC'y
Cuts at: 0 443 990 1349 1401
Size: 443 547 359 52
Fragments arranged by size:
547 443 359 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 132 164 622 654 1401
Size: 132 32 458 32 747
Fragments arranged by size:
747 458 132 32 32
HindIII A'AGCT_T
Cuts at: 0 1097 1401
Size: 1097 304
KasI G'GCGC_C
Cuts at: 0 439 1401
Size: 439 962
KpnI G_GTAC'C
Cuts at: 0 211 1401
Size: 211 1190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 1401
Size: 299 1102
MscI TGG'CCA
Cuts at: 0 200 1401
Size: 200 1201
MslI CAynn'nnrTG
Cuts at: 0 62 293 955 1401
Size: 62 231 662 446
Fragments arranged by size:
662 446 231 62
NarI GG'CG_CC
Cuts at: 0 440 1401
Size: 440 961
NcoI C'CATG_G
Cuts at: 0 211 1401
Size: 211 1190
NspI r_CATG'y
Cuts at: 0 709 960 1401
Size: 709 251 441
Fragments arranged by size:
709 441 251
PacI TTA_AT'TAA
Cuts at: 0 53 1401
Size: 53 1348
PasI CC'CwG_GG
Cuts at: 0 977 1401
Size: 977 424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 626 658 1401
Size: 626 32 743
Fragments arranged by size:
743 626 32
PpuMI rG'GwC_Cy
Cuts at: 0 855 1401
Size: 855 546
PshAI GACnn'nnGTC
Cuts at: 0 1070 1401
Size: 1070 331
PstI C_TGCA'G
Cuts at: 0 1072 1401
Size: 1072 329
SapI GCTCTTCn'nnn_
Cuts at: 0 104 1401
Size: 104 1297
SfcI C'TryA_G
Cuts at: 0 930 1068 1401
Size: 930 138 333
Fragments arranged by size:
930 333 138
SfoI GGC'GCC
Cuts at: 0 441 1401
Size: 441 960
SmlI C'TyrA_G
Cuts at: 0 712 1312 1401
Size: 712 600 89
Fragments arranged by size:
712 600 89
SspI AAT'ATT
Cuts at: 0 414 1401
Size: 414 987
StyI C'CwwG_G
Cuts at: 0 211 1401
Size: 211 1190
SwaI ATTT'AAAT
Cuts at: 0 62 1401
Size: 62 1339
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 684 847 969 1401
Size: 684 163 122 432
Fragments arranged by size:
684 432 163 122
TatI w'GTAC_w
Cuts at: 0 5 1019 1401
Size: 5 1014 382
Fragments arranged by size:
1014 382 5
Enzymes that do cut:
AccI Acc65I AfeI AhdI AlfI AloI AlwNI ApaLI
ApoI AseI AvaI BaeI BanI BanII BbeI BbsI
BlpI Bme1580I BmrI BpmI BpuEI BsaHI BsaXI BsgI
BsiHKAI Bsp1286I BspHI BsrDI BsrGI BssSI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI EaeI EarI Eco57MI EcoO109I
FalI HaeII Hin4I HindIII KasI KpnI MmeI MscI
MslI NarI NcoI NspI PacI PasI PpiI PpuMI
PshAI PstI SapI SfcI SfoI SmlI SspI StyI
SwaI TaqII TatI
Enzymes that do not cut:
AarI AatII AclI AcuI AflII AflIII AgeI AleI
ApaI AscI AsiSI AvrII BamHI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BmgBI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsaWI BseRI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrFI BssHII BstAPI
BstBI BstEII BstXI BstYI ClaI CspCI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HincII HpaI MfeI MluI MspA1I NaeI NdeI
NgoMIV NheI NotI NruI NsiI PciI PflMI PfoI
PmeI PmlI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI