Restriction map for
pF1KB0421
(Linear) (Six-base) MAPSORT of: KIBB0421.seq Check: 7897 from: 1 to: 1230
KIBB0421.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AccI GT'mk_AC
Cuts at: 0 977 1230
Size: 977 253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 1046 1230
Size: 504 542 184
Fragments arranged by size:
542 504 184
AhdI GACnn_n'nnGTC
Cuts at: 0 586 1230
Size: 586 644
AlwNI CAG_nnn'CTG
Cuts at: 0 527 1066 1230
Size: 527 539 164
Fragments arranged by size:
539 527 164
ApaI G_GGCC'C
Cuts at: 0 1143 1230
Size: 1143 87
AvaI C'yCGr_G
Cuts at: 0 133 377 1230
Size: 133 244 853
Fragments arranged by size:
853 244 133
BamHI G'GATC_C
Cuts at: 0 137 1230
Size: 137 1093
BanII G_rGCy'C
Cuts at: 0 224 512 1143 1218 1230
Size: 224 288 631 75 12
Fragments arranged by size:
631 288 224 75 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 138 839 1034 1124 1230
Size: 138 701 195 90 106
Fragments arranged by size:
701 195 138 106 90
BbvCI CC'TCA_GC
Cuts at: 0 1103 1230
Size: 1103 127
BglI GCCn_nnn'nGGC
Cuts at: 0 30 1222 1230
Size: 30 1192 8
Fragments arranged by size:
1192 30 8
Bme1580I G_kGCm'C
Cuts at: 0 1143 1230
Size: 1143 87
BmgBI CAC'GTC
Cuts at: 0 144 1230
Size: 144 1086
BmrI ACTGGGnnnn_n'
Cuts at: 0 828 837 1230
Size: 828 9 393
Fragments arranged by size:
828 393 9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 27 1230
Size: 27 1203
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 764 1230
Size: 764 466
Bpu10I CC'TnA_GC
Cuts at: 0 1103 1230
Size: 1103 127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 1021 1230
Size: 409 612 209
Fragments arranged by size:
612 409 209
BsaI GGTCTCn'nnnn_
Cuts at: 0 380 573 594 624 947 1230
Size: 380 193 21 30 323 283
Fragments arranged by size:
380 323 283 193 30 21
BsaWI w'CCGG_w
Cuts at: 0 244 1230
Size: 244 986
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 452 727 1230
Size: 103 349 275 503
Fragments arranged by size:
503 349 275 103
BseYI C'CCAG_C
Cuts at: 0 358 1081 1230
Size: 358 723 149
Fragments arranged by size:
723 358 149
BsiHKAI G_wGCw'C
Cuts at: 0 800 1230
Size: 800 430
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1203 1230
Size: 1203 27
Bsp1286I G_dGCh'C
Cuts at: 0 224 512 800 1143 1218 1230
Size: 224 288 288 343 75 12
Fragments arranged by size:
343 288 288 224 75 12
BspEI T'CCGG_A
Cuts at: 0 244 1230
Size: 244 986
BsrBI CCG'CTC
Cuts at: 0 30 1230
Size: 30 1200
BsrFI r'CCGG_y
Cuts at: 0 47 1230
Size: 47 1183
BsrGI T'GTAC_A
Cuts at: 0 729 1230
Size: 729 501
BssHII G'CGCG_C
Cuts at: 0 161 1230
Size: 161 1069
BssSI C'ACGA_G
Cuts at: 0 76 223 444 1230
Size: 76 147 221 786
Fragments arranged by size:
786 221 147 76
BstEII G'GTnAC_C
Cuts at: 0 1095 1230
Size: 1095 135
BstXI CCAn_nnnn'nTGG
Cuts at: 0 753 1230
Size: 753 477
BstYI r'GATC_y
Cuts at: 0 137 1230
Size: 137 1093
Bsu36I CC'TnA_GG
Cuts at: 0 12 479 1230
Size: 12 467 751
Fragments arranged by size:
751 467 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 673 1230
Size: 673 557
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 652 687 1230
Size: 652 35 543
Fragments arranged by size:
652 543 35
DraIII CAC_nnn'GTG
Cuts at: 0 727 1230
Size: 727 503
EaeI y'GGCC_r
Cuts at: 0 498 529 743 959 1230
Size: 498 31 214 216 271
Fragments arranged by size:
498 271 216 214 31
EarI CTCTTCn'nnn_
Cuts at: 0 479 1230
Size: 479 751
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 764 1046 1230
Size: 504 260 282 184
Fragments arranged by size:
504 282 260 184
EcoO109I rG'GnC_Cy
Cuts at: 0 270 1230
Size: 270 960
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 572 604 1230
Size: 572 32 626
Fragments arranged by size:
626 572 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 1230
Size: 305 925
MscI TGG'CCA
Cuts at: 0 531 745 961 1230
Size: 531 214 216 269
Fragments arranged by size:
531 269 216 214
MslI CAynn'nnrTG
Cuts at: 0 391 1230
Size: 391 839
MspA1I CmG'CkG
Cuts at: 0 158 738 784 1230
Size: 158 580 46 446
Fragments arranged by size:
580 446 158 46
PflMI CCAn_nnn'nTGG
Cuts at: 0 549 1230
Size: 549 681
PfoI T'CCnGG_A
Cuts at: 0 251 1230
Size: 251 979
PpuMI rG'GwC_Cy
Cuts at: 0 270 1230
Size: 270 960
PspOMI G'GGCC_C
Cuts at: 0 1139 1230
Size: 1139 91
PstI C_TGCA'G
Cuts at: 0 1160 1230
Size: 1160 70
PvuII CAG'CTG
Cuts at: 0 158 738 784 1230
Size: 158 580 46 446
Fragments arranged by size:
580 446 158 46
SbfI CC_TGCA'GG
Cuts at: 0 1160 1230
Size: 1160 70
SfcI C'TryA_G
Cuts at: 0 330 1156 1230
Size: 330 826 74
Fragments arranged by size:
826 330 74
SmlI C'TyrA_G
Cuts at: 0 424 1000 1230
Size: 424 576 230
Fragments arranged by size:
576 424 230
StyI C'CwwG_G
Cuts at: 0 746 1037 1230
Size: 746 291 193
Fragments arranged by size:
746 291 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 928 1230
Size: 928 302
TatI w'GTAC_w
Cuts at: 0 729 1230
Size: 729 501
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 192 224 435 467 629 661 1230
Size: 192 32 211 32 162 32 569
Fragments arranged by size:
569 211 192 162 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 175 600 1230
Size: 175 425 630
Fragments arranged by size:
630 425 175
Enzymes that do cut:
AccI AcuI AhdI AlwNI ApaI AvaI BamHI BanII
BbsI BbvCI BglI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaWI BseRI BseYI BsiHKAI BsmBI
Bsp1286I BspEI BsrBI BsrFI BsrGI BssHII BssSI BstEII
BstXI BstYI Bsu36I BtgZI CspCI DraIII EaeI EarI
Eco57MI EcoO109I Hin4I MmeI MscI MslI MspA1I PflMI
PfoI PpuMI PspOMI PstI PvuII SbfI SfcI SmlI
StyI TaqII TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BanI BbeI BcgI BciVI BclI BfrBI
BglII BlpI BmtI BsaAI BsaBI BsaHI BsaXI BsgI
BsiEI BsiWI BsmI BspHI BspMI BsrDI BstAPI BstBI
BstZ17I BtgI BtsI ClaI DraI DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI XbaI XcmI XhoI XmaI XmnI ZraI