Restriction map for
pF1KB0428
(Linear) (Six-base) MAPSORT of: KIBB0428.seq Check: 8826 from: 1 to: 2556
KIBB0428.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 721 2556
Size: 721 1835
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1097 1580 2043 2463 2556
Size: 1097 483 463 420 93
Fragments arranged by size:
1097 483 463 420 93
AflII C'TTAA_G
Cuts at: 0 1902 2556
Size: 1902 654
AlwNI CAG_nnn'CTG
Cuts at: 0 198 328 812 1336 1648 2556
Size: 198 130 484 524 312 908
Fragments arranged by size:
908 524 484 312 198 130
ApaLI G'TGCA_C
Cuts at: 0 736 2069 2333 2417 2556
Size: 736 1333 264 84 139
Fragments arranged by size:
1333 736 264 139 84
ApoI r'AATT_y
Cuts at: 0 1787 2157 2556
Size: 1787 370 399
Fragments arranged by size:
1787 399 370
BamHI G'GATC_C
Cuts at: 0 578 2556
Size: 578 1978
BanI G'GyrC_C
Cuts at: 0 1437 2556
Size: 1437 1119
BanII G_rGCy'C
Cuts at: 0 598 2239 2556
Size: 598 1641 317
Fragments arranged by size:
1641 598 317
BbsI GAAGACnn'nnnn_
Cuts at: 0 309 1848 2556
Size: 309 1539 708
Fragments arranged by size:
1539 708 309
BbvCI CC'TCA_GC
Cuts at: 0 1577 2556
Size: 1577 979
BciVI GTATCCnnnnn_n'
Cuts at: 0 1620 2019 2339 2556
Size: 1620 399 320 217
Fragments arranged by size:
1620 399 320 217
BfrBI ATG'CAT
Cuts at: 0 2001 2556
Size: 2001 555
BglI GCCn_nnn'nGGC
Cuts at: 0 1058 2556
Size: 1058 1498
BglII A'GATC_T
Cuts at: 0 310 585 1774 2556
Size: 310 275 1189 782
Fragments arranged by size:
1189 782 310 275
BlpI GC'TnA_GC
Cuts at: 0 848 2556
Size: 848 1708
Bme1580I G_kGCm'C
Cuts at: 0 740 2073 2337 2421 2556
Size: 740 1333 264 84 135
Fragments arranged by size:
1333 740 264 135 84
BmrI ACTGGGnnnn_n'
Cuts at: 0 750 1010 1239 1272 2556
Size: 750 260 229 33 1284
Fragments arranged by size:
1284 750 260 229 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 537 726 898 1649 2123 2556
Size: 537 189 172 751 474 433
Fragments arranged by size:
751 537 474 433 189 172
Bpu10I CC'TnA_GC
Cuts at: 0 139 856 998 1133 1577 2556
Size: 139 717 142 135 444 979
Fragments arranged by size:
979 717 444 142 139 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2223 2556
Size: 2223 333
BsaI GGTCTCn'nnnn_
Cuts at: 0 37 555 748 916 949 1237 1267 1438
Size: 37 518 193 168 33 288 30 171
Cuts at: 1438 1920 1938 2355 2556
Size: 482 18 417 201
Fragments arranged by size:
518 482 417 288 201 193 171 168
37 33 30 18
BsaAI yAC'GTr
Cuts at: 0 2416 2556
Size: 2416 140
BsaWI w'CCGG_w
Cuts at: 0 541 875 1295 2087 2556
Size: 541 334 420 792 469
Fragments arranged by size:
792 541 469 420 334
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1647 1677 1871 1901 2556
Size: 1647 30 194 30 655
Fragments arranged by size:
1647 655 194 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 274 757 1123 2556
Size: 274 483 366 1433
Fragments arranged by size:
1433 483 366 274
BseYI C'CCAG_C
Cuts at: 0 88 419 591 686 2556
Size: 88 331 172 95 1870
Fragments arranged by size:
1870 331 172 95 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1178 2556
Size: 1178 1378
BsiHKAI G_wGCw'C
Cuts at: 0 740 999 1665 2073 2239 2337 2421 2556
Size: 740 259 666 408 166 98 84 135
Fragments arranged by size:
740 666 408 259 166 135 98 84
Bsp1286I G_dGCh'C
Cuts at: 0 598 740 999 1665 2073 2239 2337 2421
Size: 598 142 259 666 408 166 98 84
Cuts at: 2421 2556
Size: 135
Fragments arranged by size:
666 598 408 259 166 142 135 98
84
BspEI T'CCGG_A
Cuts at: 0 875 1295 2556
Size: 875 420 1261
Fragments arranged by size:
1261 875 420
BspHI T'CATG_A
Cuts at: 0 2460 2556
Size: 2460 96
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 721 967 2556
Size: 721 246 1589
Fragments arranged by size:
1589 721 246
BsrBI CCG'CTC
Cuts at: 0 521 852 941 2556
Size: 521 331 89 1615
Fragments arranged by size:
1615 521 331 89
BsrDI GCAATG_nn'
Cuts at: 0 1004 1824 2556
Size: 1004 820 732
Fragments arranged by size:
1004 820 732
BsrGI T'GTAC_A
Cuts at: 0 1224 2556
Size: 1224 1332
BssSI C'ACGA_G
Cuts at: 0 274 2556
Size: 274 2282
BstAPI GCAn_nnn'nTGC
Cuts at: 0 345 2556
Size: 345 2211
BstXI CCAn_nnnn'nTGG
Cuts at: 0 835 1324 2556
Size: 835 489 1232
Fragments arranged by size:
1232 835 489
BstYI r'GATC_y
Cuts at: 0 218 310 578 585 1158 1774 2556
Size: 218 92 268 7 573 616 782
Fragments arranged by size:
782 616 573 268 218 92 7
Bsu36I CC'TnA_GG
Cuts at: 0 1860 2556
Size: 1860 696
BtgI C'CryG_G
Cuts at: 0 94 574 1686 2556
Size: 94 480 1112 870
Fragments arranged by size:
1112 870 480 94
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1079 2556
Size: 1079 1477
BtsI GCAGTG_nn'
Cuts at: 0 272 1337 2556
Size: 272 1065 1219
Fragments arranged by size:
1219 1065 272
DraI TTT'AAA
Cuts at: 0 1395 2556
Size: 1395 1161
DraIII CAC_nnn'GTG
Cuts at: 0 2019 2556
Size: 2019 537
EaeI y'GGCC_r
Cuts at: 0 5 942 1431 1512 2556
Size: 5 937 489 81 1044
Fragments arranged by size:
1044 937 489 81 5
EarI CTCTTCn'nnn_
Cuts at: 0 746 913 968 1073 1115 1235 1457 1514
Size: 746 167 55 105 42 120 222 57
Cuts at: 1514 1616 1891 2288 2556
Size: 102 275 397 268
Fragments arranged by size:
746 397 275 268 222 167 120 105
102 57 55 42
EcoICRI GAG'CTC
Cuts at: 0 2237 2556
Size: 2237 319
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 537 726 898 1097 1580 1649 2043 2123
Size: 537 189 172 199 483 69 394 80
Cuts at: 2123 2463 2556
Size: 340 93
Fragments arranged by size:
537 483 394 340 199 189 172 93
80 69
EcoO109I rG'GnC_Cy
Cuts at: 0 1179 1367 2556
Size: 1179 188 1189
Fragments arranged by size:
1189 1179 188
FspI TGC'GCA
Cuts at: 0 154 2556
Size: 154 2402
FspAI rTGC'GCAy
Cuts at: 0 154 2556
Size: 154 2402
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 302 334 570 602 981 1013 1171 1203
Size: 302 32 236 32 379 32 158 32
Cuts at: 1203 1819 1851 2556
Size: 616 32 705
Fragments arranged by size:
705 616 379 302 236 158 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1921 2556
Size: 1921 635
HindIII A'AGCT_T
Cuts at: 0 1974 2055 2307 2530 2556
Size: 1974 81 252 223 26
Fragments arranged by size:
1974 252 223 81 26
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 539 874 1363 2044 2556
Size: 75 464 335 489 681 512
Fragments arranged by size:
681 512 489 464 335 75
MscI TGG'CCA
Cuts at: 0 7 1433 1514 2556
Size: 7 1426 81 1042
Fragments arranged by size:
1426 1042 81 7
MslI CAynn'nnrTG
Cuts at: 0 2034 2330 2465 2556
Size: 2034 296 135 91
Fragments arranged by size:
2034 296 135 91
MspA1I CmG'CkG
Cuts at: 0 96 1192 1320 2556
Size: 96 1096 128 1236
Fragments arranged by size:
1236 1096 128 96
NcoI C'CATG_G
Cuts at: 0 574 2556
Size: 574 1982
NsiI A_TGCA'T
Cuts at: 0 2003 2556
Size: 2003 553
NspI r_CATG'y
Cuts at: 0 652 2556
Size: 652 1904
PasI CC'CwG_GG
Cuts at: 0 425 1642 2556
Size: 425 1217 914
Fragments arranged by size:
1217 914 425
PflMI CCAn_nnn'nTGG
Cuts at: 0 84 253 2019 2556
Size: 84 169 1766 537
Fragments arranged by size:
1766 537 169 84
PfoI T'CCnGG_A
Cuts at: 0 1889 2556
Size: 1889 667
PpuMI rG'GwC_Cy
Cuts at: 0 1179 1367 2556
Size: 1179 188 1189
Fragments arranged by size:
1189 1179 188
PsiI TTA'TAA
Cuts at: 0 2117 2556
Size: 2117 439
PvuII CAG'CTG
Cuts at: 0 1192 1320 2556
Size: 1192 128 1236
Fragments arranged by size:
1236 1192 128
SacI G_AGCT'C
Cuts at: 0 2239 2556
Size: 2239 317
SacII CC_GC'GG
Cuts at: 0 97 2556
Size: 97 2459
SapI GCTCTTCn'nnn_
Cuts at: 0 913 1073 1891 2556
Size: 913 160 818 665
Fragments arranged by size:
913 818 665 160
SexAI A'CCwGG_T
Cuts at: 0 2015 2556
Size: 2015 541
SfcI C'TryA_G
Cuts at: 0 1830 2556
Size: 1830 726
SmlI C'TyrA_G
Cuts at: 0 1902 2238 2556
Size: 1902 336 318
Fragments arranged by size:
1902 336 318
SphI G_CATG'C
Cuts at: 0 652 2556
Size: 652 1904
StyI C'CwwG_G
Cuts at: 0 158 574 2556
Size: 158 416 1982
Fragments arranged by size:
1982 416 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 306 678 962 1518 2182 2444 2556
Size: 306 372 284 556 664 262 112
Fragments arranged by size:
664 556 372 306 284 262 112
TatI w'GTAC_w
Cuts at: 0 1224 1746 2556
Size: 1224 522 810
Fragments arranged by size:
1224 810 522
Tth111I GACn'n_nGTC
Cuts at: 0 2541 2556
Size: 2541 15
XbaI T'CTAG_A
Cuts at: 0 1161 2556
Size: 1161 1395
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 85 1023 1684 2556
Size: 85 938 661 872
Fragments arranged by size:
938 872 661 85
XmnI GAAnn'nnTTC
Cuts at: 0 2056 2224 2531 2556
Size: 2056 168 307 25
Fragments arranged by size:
2056 307 168 25
Enzymes that do cut:
AarI AcuI AflII AlwNI ApaLI ApoI BamHI BanI
BanII BbsI BbvCI BciVI BfrBI BglI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII EaeI EarI
EcoICRI Eco57MI EcoO109I FspI FspAI Hin4I HincII HindIII
MmeI MscI MslI MspA1I NcoI NsiI NspI PasI
PflMI PfoI PpuMI PsiI PvuII SacI SacII SapI
SexAI SfcI SmlI SphI StyI TaqII TatI Tth111I
XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BcgI BclI BmgBI BmtI BplI
BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BsrFI BssHII
BstBI BstEII BstZ17I ClaI CspCI DrdI EagI EciI
EcoNI EcoRI EcoRV FalI FseI HaeII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PciI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI PstI PvuI RsrII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI TstI XhoI XmaI
ZraI