Restriction map for
pF1KB0432
(Linear) (Six-base) MAPSORT of: KIBB0432.seq Check: 7118 from: 1 to: 2562
KIBB0432.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1299 1401 1682 2562
Size: 1299 102 281 880
Fragments arranged by size:
1299 880 281 102
AccI GT'mk_AC
Cuts at: 0 1616 2543 2562
Size: 1616 927 19
Fragments arranged by size:
1616 927 19
Acc65I G'GTAC_C
Cuts at: 0 2448 2562
Size: 2448 114
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 916 1930 2029 2058 2347 2562
Size: 916 1014 99 29 289 215
Fragments arranged by size:
1014 916 289 215 99 29
AflIII A'CryG_T
Cuts at: 0 1919 2562
Size: 1919 643
AgeI A'CCGG_T
Cuts at: 0 1033 2562
Size: 1033 1529
AleI CACnn'nnGTG
Cuts at: 0 1034 1139 1850 2562
Size: 1034 105 711 712
Fragments arranged by size:
1034 712 711 105
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 490 522 2562
Size: 490 32 2040
Fragments arranged by size:
2040 490 32
AlwNI CAG_nnn'CTG
Cuts at: 0 91 384 609 711 1236 2341 2562
Size: 91 293 225 102 525 1105 221
Fragments arranged by size:
1105 525 293 225 221 102 91
ApaI G_GGCC'C
Cuts at: 0 475 616 2518 2562
Size: 475 141 1902 44
Fragments arranged by size:
1902 475 141 44
ApaLI G'TGCA_C
Cuts at: 0 1558 2562
Size: 1558 1004
ApoI r'AATT_y
Cuts at: 0 1357 2562
Size: 1357 1205
AvaI C'yCGr_G
Cuts at: 0 1848 2353 2562
Size: 1848 505 209
Fragments arranged by size:
1848 505 209
BanI G'GyrC_C
Cuts at: 0 741 916 1144 2349 2448 2562
Size: 741 175 228 1205 99 114
Fragments arranged by size:
1205 741 228 175 114 99
BanII G_rGCy'C
Cuts at: 0 475 616 1937 2476 2518 2562
Size: 475 141 1321 539 42 44
Fragments arranged by size:
1321 539 475 141 44 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 904 2562
Size: 904 1658
BbvCI CC'TCA_GC
Cuts at: 0 581 2462 2562
Size: 581 1881 100
Fragments arranged by size:
1881 581 100
BfrBI ATG'CAT
Cuts at: 0 504 2562
Size: 504 2058
BglII A'GATC_T
Cuts at: 0 338 2562
Size: 338 2224
BlpI GC'TnA_GC
Cuts at: 0 1501 2562
Size: 1501 1061
Bme1580I G_kGCm'C
Cuts at: 0 362 475 515 616 865 1443 1562 2088
Size: 362 113 40 101 249 578 119 526
Cuts at: 2088 2354 2518 2540 2562
Size: 266 164 22 22
Fragments arranged by size:
578 526 362 266 249 164 119 113
101 40 22 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 721 2320 2562
Size: 721 1599 242
Fragments arranged by size:
1599 721 242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 749 869 1112 1492 1684 2338 2562
Size: 46 703 120 243 380 192 654 224
Fragments arranged by size:
703 654 380 243 224 192 120 46
Bpu10I CC'TnA_GC
Cuts at: 0 581 2462 2562
Size: 581 1881 100
Fragments arranged by size:
1881 581 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 949 1363 2562
Size: 949 414 1199
Fragments arranged by size:
1199 949 414
BsaHI Gr'CG_yC
Cuts at: 0 624 1746 2562
Size: 624 1122 816
Fragments arranged by size:
1122 816 624
BsaWI w'CCGG_w
Cuts at: 0 1033 2562
Size: 1033 1529
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 516 546 1552 1582 1630 1660 2008 2038
Size: 516 30 1006 30 48 30 348 30
Cuts at: 2038 2562
Size: 524
Fragments arranged by size:
1006 524 516 348 48 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 530 533 542 1510 2259 2335 2562
Size: 530 3 9 968 749 76 227
Fragments arranged by size:
968 749 530 227 76 9 3
BseYI C'CCAG_C
Cuts at: 0 514 527 682 1192 1351 1394 2341 2469
Size: 514 13 155 510 159 43 947 128
Cuts at: 2469 2562
Size: 93
Fragments arranged by size:
947 514 510 159 155 128 93 43
13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 505 2082 2562
Size: 409 96 1577 480
Fragments arranged by size:
1577 480 409 96
BsiEI CG_ry'CG
Cuts at: 0 1999 2562
Size: 1999 563
BsiHKAI G_wGCw'C
Cuts at: 0 422 973 1478 1562 1670 2562
Size: 422 551 505 84 108 892
Fragments arranged by size:
892 551 505 422 108 84
BsmI GAATG_Cn'
Cuts at: 0 240 504 2562
Size: 240 264 2058
Fragments arranged by size:
2058 264 240
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2200 2562
Size: 2200 362
Bsp1286I G_dGCh'C
Cuts at: 0 362 422 475 515 616 865 973 1443
Size: 362 60 53 40 101 249 108 470
Cuts at: 1443 1478 1562 1670 1937 2088 2354 2476 2518
Size: 35 84 108 267 151 266 122 42
Cuts at: 2518 2540 2562
Size: 22 22
Fragments arranged by size:
470 362 267 266 249 151 122 108
108 101 84 60 53 42 40 35
22 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1016 1299 1401 1682 1748 1766 2228 2562
Size: 1016 283 102 281 66 18 462 334
Fragments arranged by size:
1016 462 334 283 281 102 66 18
BsrDI GCAATG_nn'
Cuts at: 0 1055 2562
Size: 1055 1507
BsrFI r'CCGG_y
Cuts at: 0 1033 1994 2562
Size: 1033 961 568
Fragments arranged by size:
1033 961 568
BsrGI T'GTAC_A
Cuts at: 0 659 788 1415 2562
Size: 659 129 627 1147
Fragments arranged by size:
1147 659 627 129
BssHII G'CGCG_C
Cuts at: 0 2106 2562
Size: 2106 456
BssSI C'ACGA_G
Cuts at: 0 1930 2562
Size: 1930 632
BstAPI GCAn_nnn'nTGC
Cuts at: 0 510 1674 2562
Size: 510 1164 888
Fragments arranged by size:
1164 888 510
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1311 2412 2562
Size: 1311 1101 150
Fragments arranged by size:
1311 1101 150
BstYI r'GATC_y
Cuts at: 0 338 701 2562
Size: 338 363 1861
Fragments arranged by size:
1861 363 338
Bsu36I CC'TnA_GG
Cuts at: 0 1655 2562
Size: 1655 907
BtgI C'CryG_G
Cuts at: 0 1761 2562
Size: 1761 801
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 425 2562
Size: 425 2137
BtsI GCAGTG_nn'
Cuts at: 0 559 1707 2277 2562
Size: 559 1148 570 285
Fragments arranged by size:
1148 570 559 285
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 618 653 2562
Size: 618 35 1909
Fragments arranged by size:
1909 618 35
DraIII CAC_nnn'GTG
Cuts at: 0 1119 2562
Size: 1119 1443
DrdI GACnn_nn'nnGTC
Cuts at: 0 1612 2562
Size: 1612 950
EaeI y'GGCC_r
Cuts at: 0 75 248 263 869 1065 1996 2562
Size: 75 173 15 606 196 931 566
Fragments arranged by size:
931 606 566 196 173 75 15
EagI C'GGCC_G
Cuts at: 0 1996 2562
Size: 1996 566
EarI CTCTTCn'nnn_
Cuts at: 0 1277 1906 2562
Size: 1277 629 656
Fragments arranged by size:
1277 656 629
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2506 2562
Size: 2506 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 749 869 916 1112 1492 1684 1930
Size: 46 703 120 47 196 380 192 246
Cuts at: 1930 2029 2058 2338 2347 2562
Size: 99 29 280 9 215
Fragments arranged by size:
703 380 280 246 215 196 192 120
99 47 46 29 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 388 2562
Size: 388 2174
EcoO109I rG'GnC_Cy
Cuts at: 0 21 315 471 472 613 1659 2217 2514
Size: 21 294 156 1 141 1046 558 297
Cuts at: 2514 2562
Size: 48
Fragments arranged by size:
1046 558 297 294 156 141 48 21
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1821 2394 2562
Size: 1821 573 168
Fragments arranged by size:
1821 573 168
HaeII r_GCGC'y
Cuts at: 0 854 2562
Size: 854 1708
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1522 1554 1671 1703 2562
Size: 1522 32 117 32 859
Fragments arranged by size:
1522 859 117 32 32
HincII GTy'rAC
Cuts at: 0 1638 2562
Size: 1638 924
KpnI G_GTAC'C
Cuts at: 0 2452 2562
Size: 2452 110
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 2501 2562
Size: 121 2380 61
Fragments arranged by size:
2380 121 61
MscI TGG'CCA
Cuts at: 0 77 250 265 871 2562
Size: 77 173 15 606 1691
Fragments arranged by size:
1691 606 173 77 15
MslI CAynn'nnrTG
Cuts at: 0 355 403 1034 1139 1403 1850 2410 2562
Size: 355 48 631 105 264 447 560 152
Fragments arranged by size:
631 560 447 355 264 152 105 48
MspA1I CmG'CkG
Cuts at: 0 304 1273 1511 1769 2135 2189 2562
Size: 304 969 238 258 366 54 373
Fragments arranged by size:
969 373 366 304 258 238 54
NsiI A_TGCA'T
Cuts at: 0 506 2562
Size: 506 2056
NspI r_CATG'y
Cuts at: 0 504 1064 1107 1837 1923 2562
Size: 504 560 43 730 86 639
Fragments arranged by size:
730 639 560 504 86 43
PasI CC'CwG_GG
Cuts at: 0 468 619 2562
Size: 468 151 1943
Fragments arranged by size:
1943 468 151
PciI A'CATG_T
Cuts at: 0 1919 2562
Size: 1919 643
PflMI CCAn_nnn'nTGG
Cuts at: 0 138 384 609 711 756 2562
Size: 138 246 225 102 45 1806
Fragments arranged by size:
1806 246 225 138 102 45
PfoI T'CCnGG_A
Cuts at: 0 929 1128 2562
Size: 929 199 1434
Fragments arranged by size:
1434 929 199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1873 1905 2562
Size: 1873 32 657
Fragments arranged by size:
1873 657 32
PpuMI rG'GwC_Cy
Cuts at: 0 2217 2562
Size: 2217 345
PspOMI G'GGCC_C
Cuts at: 0 471 612 2514 2562
Size: 471 141 1902 48
Fragments arranged by size:
1902 471 141 48
PstI C_TGCA'G
Cuts at: 0 942 1013 1679 1981 2501 2562
Size: 942 71 666 302 520 61
Fragments arranged by size:
942 666 520 302 71 61
PvuII CAG'CTG
Cuts at: 0 1273 2562
Size: 1273 1289
ScaI AGT'ACT
Cuts at: 0 1567 2562
Size: 1567 995
SfcI C'TryA_G
Cuts at: 0 938 1009 1675 1977 2497 2562
Size: 938 71 666 302 520 65
Fragments arranged by size:
938 666 520 302 71 65
SgrAI Cr'CCGG_yG
Cuts at: 0 1033 2562
Size: 1033 1529
SmlI C'TyrA_G
Cuts at: 0 964 1378 2562
Size: 964 414 1184
Fragments arranged by size:
1184 964 414
SphI G_CATG'C
Cuts at: 0 1837 2562
Size: 1837 725
StuI AGG'CCT
Cuts at: 0 1261 2554 2562
Size: 1261 1293 8
Fragments arranged by size:
1293 1261 8
StyI C'CwwG_G
Cuts at: 0 78 2150 2562
Size: 78 2072 412
Fragments arranged by size:
2072 412 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 312 541 1774 2404 2562
Size: 312 229 1233 630 158
Fragments arranged by size:
1233 630 312 229 158
TatI w'GTAC_w
Cuts at: 0 659 788 1415 1565 2562
Size: 659 129 627 150 997
Fragments arranged by size:
997 659 627 150 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 516 548 1552 1584 2008 2040 2562
Size: 516 32 1004 32 424 32 522
Fragments arranged by size:
1004 522 516 424 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 258 2484 2562
Size: 258 2226 78
Fragments arranged by size:
2226 258 78
XmnI GAAnn'nnTTC
Cuts at: 0 181 1045 1804 2562
Size: 181 864 759 758
Fragments arranged by size:
864 759 758 181
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AgeI AleI AloI
AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbsI
BbvCI BfrBI BglII BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KpnI
MmeI MscI MslI MspA1I NsiI NspI PasI PciI
PflMI PfoI PpiI PpuMI PspOMI PstI PvuII ScaI
SfcI SgrAI SmlI SphI StuI StyI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AhdI AlfI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI
BglI BmgBI BmtI BplI BsaI BsaAI BsaBI BsiWI
BspEI BspHI BsrBI BstBI BstEII BstZ17I ClaI DraI
EcoICRI EcoRI EcoRV FseI FspI FspAI HindIII HpaI
KasI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SmaI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmaI
ZraI