Restriction map for
pF1KB0434
(Linear) (Six-base) MAPSORT of: KIBB0434.seq Check: 8617 from: 1 to: 2598
KIBB0434.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 406 1403 2597 2598
Size: 250 156 997 1194 1
Fragments arranged by size:
1194 997 250 156 1
AflII C'TTAA_G
Cuts at: 0 348 1513 2598
Size: 348 1165 1085
Fragments arranged by size:
1165 1085 348
AflIII A'CryG_T
Cuts at: 0 1757 2401 2598
Size: 1757 644 197
Fragments arranged by size:
1757 644 197
AlwNI CAG_nnn'CTG
Cuts at: 0 2575 2598
Size: 2575 23
ApoI r'AATT_y
Cuts at: 0 91 121 517 833 948 1431 1498 1975
Size: 91 30 396 316 115 483 67 477
Cuts at: 1975 2244 2271 2465 2598
Size: 269 27 194 133
Fragments arranged by size:
483 477 396 316 269 194 133 115
91 67 30 27
AseI AT'TA_AT
Cuts at: 0 1219 2598
Size: 1219 1379
AvrII C'CTAG_G
Cuts at: 0 2439 2598
Size: 2439 159
BanI G'GyrC_C
Cuts at: 0 873 2598
Size: 873 1725
BanII G_rGCy'C
Cuts at: 0 1806 2598
Size: 1806 792
BbsI GAAGACnn'nnnn_
Cuts at: 0 966 2598
Size: 966 1632
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 694 728 2598
Size: 694 34 1870
Fragments arranged by size:
1870 694 34
BglI GCCn_nnn'nGGC
Cuts at: 0 882 2598
Size: 882 1716
BglII A'GATC_T
Cuts at: 0 384 1688 2598
Size: 384 1304 910
Fragments arranged by size:
1304 910 384
Bme1580I G_kGCm'C
Cuts at: 0 1476 2598
Size: 1476 1122
BmrI ACTGGGnnnn_n'
Cuts at: 0 780 2598
Size: 780 1818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2380 2598
Size: 2380 218
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 409 787 2598
Size: 72 337 378 1811
Fragments arranged by size:
1811 378 337 72
BsaI GGTCTCn'nnnn_
Cuts at: 0 616 2598
Size: 616 1982
BsaAI yAC'GTr
Cuts at: 0 1786 2598
Size: 1786 812
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1912 2598
Size: 1912 686
BsiEI CG_ry'CG
Cuts at: 0 8 2598
Size: 8 2590
BsiHKAI G_wGCw'C
Cuts at: 0 1396 1806 2598
Size: 1396 410 792
Fragments arranged by size:
1396 792 410
BsmI GAATG_Cn'
Cuts at: 0 36 2598
Size: 36 2562
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2146 2598
Size: 2146 452
Bsp1286I G_dGCh'C
Cuts at: 0 1396 1476 1806 2598
Size: 1396 80 330 792
Fragments arranged by size:
1396 792 330 80
BspHI T'CATG_A
Cuts at: 0 1524 1704 2124 2598
Size: 1524 180 420 474
Fragments arranged by size:
1524 474 420 180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 441 598 2598
Size: 441 157 2000
Fragments arranged by size:
2000 441 157
BsrDI GCAATG_nn'
Cuts at: 0 1496 2281 2598
Size: 1496 785 317
Fragments arranged by size:
1496 785 317
BsrFI r'CCGG_y
Cuts at: 0 3 1067 2598
Size: 3 1064 1531
Fragments arranged by size:
1531 1064 3
BssSI C'ACGA_G
Cuts at: 0 1234 2598
Size: 1234 1364
BstAPI GCAn_nnn'nTGC
Cuts at: 0 962 1678 2598
Size: 962 716 920
Fragments arranged by size:
962 920 716
BstXI CCAn_nnnn'nTGG
Cuts at: 0 870 2598
Size: 870 1728
BstYI r'GATC_y
Cuts at: 0 47 384 1688 2325 2598
Size: 47 337 1304 637 273
Fragments arranged by size:
1304 637 337 273 47
Bsu36I CC'TnA_GG
Cuts at: 0 940 2598
Size: 940 1658
BtsI GCAGTG_nn'
Cuts at: 0 1453 2162 2598
Size: 1453 709 436
Fragments arranged by size:
1453 709 436
DraI TTT'AAA
Cuts at: 0 918 2598
Size: 918 1680
EaeI y'GGCC_r
Cuts at: 0 5 1940 2585 2598
Size: 5 1935 645 13
Fragments arranged by size:
1935 645 13 5
EagI C'GGCC_G
Cuts at: 0 5 2598
Size: 5 2593
EarI CTCTTCn'nnn_
Cuts at: 0 43 226 1022 1794 1823 2525 2598
Size: 43 183 796 772 29 702 73
Fragments arranged by size:
796 772 702 183 73 43 29
EcoICRI GAG'CTC
Cuts at: 0 1804 2598
Size: 1804 794
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 406 1403 2380 2597 2598
Size: 250 156 997 977 217 1
Fragments arranged by size:
997 977 250 217 156 1
EcoO109I rG'GnC_Cy
Cuts at: 0 354 2598
Size: 354 2244
EcoRI G'AATT_C
Cuts at: 0 2271 2598
Size: 2271 327
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 66 355 712 1657 1939 2598
Size: 66 289 357 945 282 659
Fragments arranged by size:
945 659 357 289 282 66
FspI TGC'GCA
Cuts at: 0 437 2598
Size: 437 2161
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 843 875 1893 1925 2004 2036 2598
Size: 843 32 1018 32 79 32 562
Fragments arranged by size:
1018 843 562 79 32 32 32
HindIII A'AGCT_T
Cuts at: 0 191 623 1699 2488 2598
Size: 191 432 1076 789 110
Fragments arranged by size:
1076 789 432 191 110
MscI TGG'CCA
Cuts at: 0 1942 2587 2598
Size: 1942 645 11
Fragments arranged by size:
1942 645 11
MslI CAynn'nnrTG
Cuts at: 0 254 321 2598
Size: 254 67 2277
Fragments arranged by size:
2277 254 67
MspA1I CmG'CkG
Cuts at: 0 1423 2302 2598
Size: 1423 879 296
Fragments arranged by size:
1423 879 296
NaeI GCC'GGC
Cuts at: 0 5 2598
Size: 5 2593
NdeI CA'TA_TG
Cuts at: 0 1611 2598
Size: 1611 987
NgoMIV G'CCGG_C
Cuts at: 0 3 2598
Size: 3 2595
NspI r_CATG'y
Cuts at: 0 1261 1356 1455 1761 2405 2598
Size: 1261 95 99 306 644 193
Fragments arranged by size:
1261 644 306 193 99 95
PasI CC'CwG_GG
Cuts at: 0 863 2598
Size: 863 1735
PciI A'CATG_T
Cuts at: 0 1757 2401 2598
Size: 1757 644 197
Fragments arranged by size:
1757 644 197
PflMI CCAn_nnn'nTGG
Cuts at: 0 1308 2598
Size: 1308 1290
PfoI T'CCnGG_A
Cuts at: 0 2520 2598
Size: 2520 78
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1217 1249 2598
Size: 1217 32 1349
Fragments arranged by size:
1349 1217 32
PpuMI rG'GwC_Cy
Cuts at: 0 354 2598
Size: 354 2244
PsiI TTA'TAA
Cuts at: 0 1484 2598
Size: 1484 1114
PstI C_TGCA'G
Cuts at: 0 732 2157 2571 2598
Size: 732 1425 414 27
Fragments arranged by size:
1425 732 414 27
PvuII CAG'CTG
Cuts at: 0 2302 2598
Size: 2302 296
SacI G_AGCT'C
Cuts at: 0 1806 2598
Size: 1806 792
SapI GCTCTTCn'nnn_
Cuts at: 0 43 1794 2598
Size: 43 1751 804
Fragments arranged by size:
1751 804 43
ScaI AGT'ACT
Cuts at: 0 1132 2598
Size: 1132 1466
SfcI C'TryA_G
Cuts at: 0 367 615 674 728 1682 2153 2348 2567
Size: 367 248 59 54 954 471 195 219
Cuts at: 2567 2598
Size: 31
Fragments arranged by size:
954 471 367 248 219 195 59 54
31
SmlI C'TyrA_G
Cuts at: 0 51 348 388 802 1513 2598
Size: 51 297 40 414 711 1085
Fragments arranged by size:
1085 711 414 297 51 40
SnaBI TAC'GTA
Cuts at: 0 1786 2598
Size: 1786 812
SphI G_CATG'C
Cuts at: 0 1261 2598
Size: 1261 1337
SspI AAT'ATT
Cuts at: 0 1269 2598
Size: 1269 1329
StuI AGG'CCT
Cuts at: 0 1306 2598
Size: 1306 1292
StyI C'CwwG_G
Cuts at: 0 23 143 1307 1943 2439 2598
Size: 23 120 1164 636 496 159
Fragments arranged by size:
1164 636 496 159 120 23
TatI w'GTAC_w
Cuts at: 0 1130 1184 1207 1638 2598
Size: 1130 54 23 431 960
Fragments arranged by size:
1130 960 431 54 23
XmnI GAAnn'nnTTC
Cuts at: 0 203 1229 1925 2060 2258 2598
Size: 203 1026 696 135 198 340
Fragments arranged by size:
1026 696 340 203 198 135
Enzymes that do cut:
AcuI AflII AflIII AlwNI ApoI AseI AvrII BanI
BanII BbsI BcgI BglI BglII Bme1580I BmrI BpmI
BpuEI BsaI BsaAI BseRI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrDI BsrFI BssSI BstAPI BstXI
BstYI Bsu36I BtsI DraI EaeI EagI EarI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI Hin4I HindIII MscI
MslI MspA1I NaeI NdeI NgoMIV NspI PasI PciI
PflMI PfoI PpiI PpuMI PsiI PstI PvuII SacI
SapI ScaI SfcI SmlI SnaBI SphI SspI StuI
StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BlpI
BmgBI BmtI BplI Bpu10I BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiWI BspEI BsrBI BsrGI BssHII BstBI
BstEII BstZ17I BtgI BtgZI ClaI CspCI DraIII DrdI
EciI EcoNI EcoRV FseI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MmeI NarI NcoI NheI
NotI NruI NsiI PacI PmeI PmlI PshAI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SrfI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI
ZraI