Restriction map for
pF1KB0435
(Linear) (Six-base) MAPSORT of: KIBB0435.seq Check: 9987 from: 1 to: 1344
KIBB0435.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1326 1344
Size: 1326 18
AccI GT'mk_AC
Cuts at: 0 851 1344
Size: 851 493
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 652 1168 1344
Size: 440 212 516 176
Fragments arranged by size:
516 440 212 176
AflIII A'CryG_T
Cuts at: 0 1004 1344
Size: 1004 340
AlwNI CAG_nnn'CTG
Cuts at: 0 837 1344
Size: 837 507
ApoI r'AATT_y
Cuts at: 0 760 1344
Size: 760 584
AvaI C'yCGr_G
Cuts at: 0 71 486 672 1057 1344
Size: 71 415 186 385 287
Fragments arranged by size:
415 385 287 186 71
BanI G'GyrC_C
Cuts at: 0 187 420 481 1121 1344
Size: 187 233 61 640 223
Fragments arranged by size:
640 233 223 187 61
BanII G_rGCy'C
Cuts at: 0 167 869 889 1344
Size: 167 702 20 455
Fragments arranged by size:
702 455 167 20
BbeI G_GCGC'C
Cuts at: 0 1125 1344
Size: 1125 219
BbsI GAAGACnn'nnnn_
Cuts at: 0 640 1069 1344
Size: 640 429 275
Fragments arranged by size:
640 429 275
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 793 827 979 1013 1344
Size: 793 34 152 34 331
Fragments arranged by size:
793 331 152 34 34
BclI T'GATC_A
Cuts at: 0 563 1344
Size: 563 781
BglI GCCn_nnn'nGGC
Cuts at: 0 353 1344
Size: 353 991
BlpI GC'TnA_GC
Cuts at: 0 66 838 1078 1344
Size: 66 772 240 266
Fragments arranged by size:
772 266 240 66
Bme1580I G_kGCm'C
Cuts at: 0 142 1344
Size: 142 1202
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 767 1315 1344
Size: 388 379 548 29
Fragments arranged by size:
548 388 379 29
Bpu10I CC'TnA_GC
Cuts at: 0 1226 1344
Size: 1226 118
BsaAI yAC'GTr
Cuts at: 0 1088 1344
Size: 1088 256
BsaHI Gr'CG_yC
Cuts at: 0 28 264 1122 1344
Size: 28 236 858 222
Fragments arranged by size:
858 236 222 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 489 519 765 795 1264 1294 1344
Size: 489 30 246 30 469 30 50
Fragments arranged by size:
489 469 246 50 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 175 724 877 1344
Size: 175 549 153 467
Fragments arranged by size:
549 467 175 153
BseYI C'CCAG_C
Cuts at: 0 651 1325 1344
Size: 651 674 19
Fragments arranged by size:
674 651 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 1267 1304 1344
Size: 3 1264 37 40
Fragments arranged by size:
1264 40 37 3
BsiHKAI G_wGCw'C
Cuts at: 0 67 1344
Size: 67 1277
BsmI GAATG_Cn'
Cuts at: 0 1079 1344
Size: 1079 265
Bsp1286I G_dGCh'C
Cuts at: 0 67 142 167 869 889 1344
Size: 67 75 25 702 20 455
Fragments arranged by size:
702 455 75 67 25 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1326 1344
Size: 1326 18
BsrBI CCG'CTC
Cuts at: 0 76 1257 1344
Size: 76 1181 87
Fragments arranged by size:
1181 87 76
BsrDI GCAATG_nn'
Cuts at: 0 566 578 1344
Size: 566 12 766
Fragments arranged by size:
766 566 12
BstAPI GCAn_nnn'nTGC
Cuts at: 0 401 1344
Size: 401 943
BstYI r'GATC_y
Cuts at: 0 983 1344
Size: 983 361
BtgI C'CryG_G
Cuts at: 0 1034 1344
Size: 1034 310
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 582 1344
Size: 582 762
BtsI GCAGTG_nn'
Cuts at: 0 554 1344
Size: 554 790
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 990 1025 1344
Size: 990 35 319
Fragments arranged by size:
990 319 35
EaeI y'GGCC_r
Cuts at: 0 551 593 923 1344
Size: 551 42 330 421
Fragments arranged by size:
551 421 330 42
EarI CTCTTCn'nnn_
Cuts at: 0 673 1144 1344
Size: 673 471 200
Fragments arranged by size:
673 471 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 241 1344
Size: 241 1103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 440 652 767 1168 1315 1344
Size: 388 52 212 115 401 147 29
Fragments arranged by size:
401 388 212 147 115 52 29
EcoO109I rG'GnC_Cy
Cuts at: 0 723 1344
Size: 723 621
HaeII r_GCGC'y
Cuts at: 0 1125 1344
Size: 1125 219
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 765 797 802 834 1344
Size: 765 32 5 32 510
Fragments arranged by size:
765 510 32 32 5
HincII GTy'rAC
Cuts at: 0 852 1344
Size: 852 492
KasI G'GCGC_C
Cuts at: 0 1121 1344
Size: 1121 223
MscI TGG'CCA
Cuts at: 0 553 595 925 1344
Size: 553 42 330 419
Fragments arranged by size:
553 419 330 42
MslI CAynn'nnrTG
Cuts at: 0 571 616 1344
Size: 571 45 728
Fragments arranged by size:
728 571 45
MspA1I CmG'CkG
Cuts at: 0 834 1344
Size: 834 510
NarI GG'CG_CC
Cuts at: 0 1122 1344
Size: 1122 222
NspI r_CATG'y
Cuts at: 0 366 1185 1336 1344
Size: 366 819 151 8
Fragments arranged by size:
819 366 151 8
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 799 831 1344
Size: 799 32 513
Fragments arranged by size:
799 513 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 91 123 1344
Size: 91 32 1221
Fragments arranged by size:
1221 91 32
PvuII CAG'CTG
Cuts at: 0 834 1344
Size: 834 510
SalI G'TCGA_C
Cuts at: 0 850 1344
Size: 850 494
SapI GCTCTTCn'nnn_
Cuts at: 0 673 1344
Size: 673 671
ScaI AGT'ACT
Cuts at: 0 106 1344
Size: 106 1238
SfcI C'TryA_G
Cuts at: 0 1215 1344
Size: 1215 129
SfoI GGC'GCC
Cuts at: 0 1123 1344
Size: 1123 221
SmaI CCC'GGG
Cuts at: 0 674 1344
Size: 674 670
SmlI C'TyrA_G
Cuts at: 0 1057 1344
Size: 1057 287
SphI G_CATG'C
Cuts at: 0 1185 1344
Size: 1185 159
StyI C'CwwG_G
Cuts at: 0 434 540 1344
Size: 434 106 804
Fragments arranged by size:
804 434 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 393 1344
Size: 393 951
TatI w'GTAC_w
Cuts at: 0 104 1344
Size: 104 1240
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 489 521 1264 1296 1344
Size: 489 32 743 32 48
Fragments arranged by size:
743 489 48 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 441 588 1344
Size: 441 147 756
Fragments arranged by size:
756 441 147
XhoI C'TCGA_G
Cuts at: 0 1057 1344
Size: 1057 287
XmaI C'CCGG_G
Cuts at: 0 672 1344
Size: 672 672
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI ApoI AvaI BanI
BanII BbeI BbsI BcgI BclI BglI BlpI Bme1580I
BpmI Bpu10I BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BstAPI BstYI
BtgI BtgZI BtsI CspCI EaeI EarI EciI Eco57MI
EcoO109I HaeII Hin4I HincII KasI MscI MslI MspA1I
NarI NspI PpiI PsrI PvuII SalI SapI ScaI
SfcI SfoI SmaI SmlI SphI StyI TaqII TatI
TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BciVI BfrBI BglII BmgBI BmrI
BmtI BplI BpuEI BsaI BsaBI BsaWI BsiEI BsiWI
BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I ClaI DraI DraIII DrdI
EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI MmeI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI RsrII SacI SacII
SanDI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI
SrfI SspI StuI SwaI Tth111I XbaI XmnI ZraI