Restriction map for
pF1KB0438
(Linear) (Six-base) MAPSORT of: KIBB0438.seq Check: 2875 from: 1 to: 2607
KIBB0438.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 990 1049 2352 2607
Size: 990 59 1303 255
Fragments arranged by size:
1303 990 255 59
AatII G_ACGT'C
Cuts at: 0 65 2607
Size: 65 2542
AccI GT'mk_AC
Cuts at: 0 689 2607
Size: 689 1918
Acc65I G'GTAC_C
Cuts at: 0 269 2607
Size: 269 2338
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1453 1939 2607
Size: 1453 486 668
Fragments arranged by size:
1453 668 486
AflIII A'CryG_T
Cuts at: 0 1308 2607
Size: 1308 1299
AgeI A'CCGG_T
Cuts at: 0 272 2607
Size: 272 2335
AhdI GACnn_n'nnGTC
Cuts at: 0 1697 2607
Size: 1697 910
AleI CACnn'nnGTG
Cuts at: 0 553 1219 2607
Size: 553 666 1388
Fragments arranged by size:
1388 666 553
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2020 2607
Size: 2020 587
AlwNI CAG_nnn'CTG
Cuts at: 0 1329 1512 1548 1917 1989 2034 2073 2607
Size: 1329 183 36 369 72 45 39 534
Fragments arranged by size:
1329 534 369 183 72 45 39 36
ApaI G_GGCC'C
Cuts at: 0 538 1246 2607
Size: 538 708 1361
Fragments arranged by size:
1361 708 538
ApoI r'AATT_y
Cuts at: 0 384 2607
Size: 384 2223
AvaI C'yCGr_G
Cuts at: 0 245 290 529 1045 1071 1190 2060 2125
Size: 245 45 239 516 26 119 870 65
Cuts at: 2125 2227 2607
Size: 102 380
Fragments arranged by size:
870 516 380 245 239 119 102 65
45 26
BamHI G'GATC_C
Cuts at: 0 2298 2607
Size: 2298 309
BanI G'GyrC_C
Cuts at: 0 269 703 1422 1575 2607
Size: 269 434 719 153 1032
Fragments arranged by size:
1032 719 434 269 153
BanII G_rGCy'C
Cuts at: 0 538 1096 1246 2095 2228 2607
Size: 538 558 150 849 133 379
Fragments arranged by size:
849 558 538 379 150 133
BbeI G_GCGC'C
Cuts at: 0 707 2607
Size: 707 1900
BbsI GAAGACnn'nnnn_
Cuts at: 0 430 1773 2607
Size: 430 1343 834
Fragments arranged by size:
1343 834 430
BbvCI CC'TCA_GC
Cuts at: 0 499 912 1030 1728 2607
Size: 499 413 118 698 879
Fragments arranged by size:
879 698 499 413 118
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1271 1305 2607
Size: 1271 34 1302
Fragments arranged by size:
1302 1271 34
BfrBI ATG'CAT
Cuts at: 0 2404 2607
Size: 2404 203
BglI GCCn_nnn'nGGC
Cuts at: 0 1250 1589 2607
Size: 1250 339 1018
Fragments arranged by size:
1250 1018 339
Bme1580I G_kGCm'C
Cuts at: 0 147 538 550 1246 1337 1580 1940 2607
Size: 147 391 12 696 91 243 360 667
Fragments arranged by size:
696 667 391 360 243 147 91 12
BmgBI CAC'GTC
Cuts at: 0 551 2607
Size: 551 2056
BmrI ACTGGGnnnn_n'
Cuts at: 0 644 666 1317 1377 1593 2607
Size: 644 22 651 60 216 1014
Fragments arranged by size:
1014 651 644 216 60 22
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2086 2607
Size: 2086 521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 415 436 719 775 1323 1666 1797
Size: 400 15 21 283 56 548 343 131
Cuts at: 1797 2505 2607
Size: 708 102
Fragments arranged by size:
708 548 400 343 283 131 102 56
21 15
Bpu10I CC'TnA_GC
Cuts at: 0 499 912 1030 1728 2607
Size: 499 413 118 698 879
Fragments arranged by size:
879 698 499 413 118
BsaI GGTCTCn'nnnn_
Cuts at: 0 1126 2078 2607
Size: 1126 952 529
Fragments arranged by size:
1126 952 529
BsaHI Gr'CG_yC
Cuts at: 0 62 704 1570 2417 2607
Size: 62 642 866 847 190
Fragments arranged by size:
866 847 642 190 62
BsaWI w'CCGG_w
Cuts at: 0 272 1216 2397 2582 2607
Size: 272 944 1181 185 25
Fragments arranged by size:
1181 944 272 185 25
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2077 2107 2302 2332 2487 2517 2607
Size: 2077 30 195 30 155 30 90
Fragments arranged by size:
2077 195 155 90 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 601 900 1143 1507 1937 2607
Size: 601 299 243 364 430 670
Fragments arranged by size:
670 601 430 364 299 243
BseYI C'CCAG_C
Cuts at: 0 10 119 1495 1831 1989 2034 2261 2543
Size: 10 109 1376 336 158 45 227 282
Cuts at: 2543 2607
Size: 64
Fragments arranged by size:
1376 336 282 227 158 109 64 45
10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 565 615 1525 1534 2105 2607
Size: 105 460 50 910 9 571 502
Fragments arranged by size:
910 571 502 460 105 50 9
BsiHKAI G_wGCw'C
Cuts at: 0 1754 2045 2607
Size: 1754 291 562
Fragments arranged by size:
1754 562 291
BsmBI CGTCTCn'nnnn_
Cuts at: 0 89 338 2607
Size: 89 249 2269
Fragments arranged by size:
2269 249 89
Bsp1286I G_dGCh'C
Cuts at: 0 147 538 550 1096 1246 1337 1580 1754
Size: 147 391 12 546 150 91 243 174
Cuts at: 1754 1940 2045 2095 2228 2607
Size: 186 105 50 133 379
Fragments arranged by size:
546 391 379 243 186 174 150 147
133 105 91 50 12
BspEI T'CCGG_A
Cuts at: 0 2397 2582 2607
Size: 2397 185 25
Fragments arranged by size:
2397 185 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 173 260 886 990 1049 2352 2607
Size: 173 87 626 104 59 1303 255
Fragments arranged by size:
1303 626 255 173 104 87 59
BsrBI CCG'CTC
Cuts at: 0 403 932 1070 1461 2607
Size: 403 529 138 391 1146
Fragments arranged by size:
1146 529 403 391 138
BsrDI GCAATG_nn'
Cuts at: 0 1001 2607
Size: 1001 1606
BsrFI r'CCGG_y
Cuts at: 0 40 177 272 323 1900 2607
Size: 40 137 95 51 1577 707
Fragments arranged by size:
1577 707 137 95 51 40
BssHII G'CGCG_C
Cuts at: 0 641 2607
Size: 641 1966
BssSI C'ACGA_G
Cuts at: 0 583 1138 2607
Size: 583 555 1469
Fragments arranged by size:
1469 583 555
BstAPI GCAn_nnn'nTGC
Cuts at: 0 996 1512 1548 2073 2607
Size: 996 516 36 525 534
Fragments arranged by size:
996 534 525 516 36
BstEII G'GTnAC_C
Cuts at: 0 1207 2607
Size: 1207 1400
BstXI CCAn_nnnn'nTGG
Cuts at: 0 766 1701 2607
Size: 766 935 906
Fragments arranged by size:
935 906 766
BstYI r'GATC_y
Cuts at: 0 1655 2298 2607
Size: 1655 643 309
Fragments arranged by size:
1655 643 309
Bsu36I CC'TnA_GG
Cuts at: 0 1097 2607
Size: 1097 1510
BtgI C'CryG_G
Cuts at: 0 261 1016 1202 1443 2054 2607
Size: 261 755 186 241 611 553
Fragments arranged by size:
755 611 553 261 241 186
BtsI GCAGTG_nn'
Cuts at: 0 145 1178 1895 2607
Size: 145 1033 717 712
Fragments arranged by size:
1033 717 712 145
ClaI AT'CG_AT
Cuts at: 0 218 2607
Size: 218 2389
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 983 1018 2607
Size: 983 35 1589
Fragments arranged by size:
1589 983 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2265 2607
Size: 2265 342
EaeI y'GGCC_r
Cuts at: 0 923 1055 2289 2338 2456 2607
Size: 923 132 1234 49 118 151
Fragments arranged by size:
1234 923 151 132 118 49
EarI CTCTTCn'nnn_
Cuts at: 0 1161 1370 1610 1759 2012 2607
Size: 1161 209 240 149 253 595
Fragments arranged by size:
1161 595 253 240 209 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 10 1475 1786 2607
Size: 10 1465 311 821
Fragments arranged by size:
1465 821 311 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 415 436 719 775 1323 1453 1666
Size: 400 15 21 283 56 548 130 213
Cuts at: 1666 1797 1939 2505 2607
Size: 131 142 566 102
Fragments arranged by size:
566 548 400 283 213 142 131 130
102 56 21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 698 988 2607
Size: 698 290 1619
Fragments arranged by size:
1619 698 290
EcoO109I rG'GnC_Cy
Cuts at: 0 162 228 249 456 534 535 796 843
Size: 162 66 21 207 78 1 261 47
Cuts at: 843 1125 1242 1591 1923 2216 2607
Size: 282 117 349 332 293 391
Fragments arranged by size:
391 349 332 293 282 261 207 162
117 78 66 47 21 1
FspI TGC'GCA
Cuts at: 0 1184 2607
Size: 1184 1423
HaeII r_GCGC'y
Cuts at: 0 516 707 1413 1680 1735 2160 2256 2607
Size: 516 191 706 267 55 425 96 351
Fragments arranged by size:
706 516 425 351 267 191 96 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1683 1715 2077 2109 2302 2334 2607
Size: 1683 32 362 32 193 32 273
Fragments arranged by size:
1683 362 273 193 32 32 32
HincII GTy'rAC
Cuts at: 0 2393 2607
Size: 2393 214
KasI G'GCGC_C
Cuts at: 0 703 2607
Size: 703 1904
KpnI G_GTAC'C
Cuts at: 0 273 2607
Size: 273 2334
MscI TGG'CCA
Cuts at: 0 1057 2291 2340 2458 2607
Size: 1057 1234 49 118 149
Fragments arranged by size:
1234 1057 149 118 49
MslI CAynn'nnrTG
Cuts at: 0 553 1219 2607
Size: 553 666 1388
Fragments arranged by size:
1388 666 553
MspA1I CmG'CkG
Cuts at: 0 89 263 612 684 1499 1539 1545 2031
Size: 89 174 349 72 815 40 6 486
Cuts at: 2031 2265 2433 2607
Size: 234 168 174
Fragments arranged by size:
815 486 349 234 174 174 168 89
72 40 6
NaeI GCC'GGC
Cuts at: 0 179 1902 2607
Size: 179 1723 705
Fragments arranged by size:
1723 705 179
NarI GG'CG_CC
Cuts at: 0 704 2607
Size: 704 1903
NcoI C'CATG_G
Cuts at: 0 1443 2054 2607
Size: 1443 611 553
Fragments arranged by size:
1443 611 553
NgoMIV G'CCGG_C
Cuts at: 0 177 1900 2607
Size: 177 1723 707
Fragments arranged by size:
1723 707 177
NsiI A_TGCA'T
Cuts at: 0 2406 2607
Size: 2406 201
NspI r_CATG'y
Cuts at: 0 154 821 2408 2607
Size: 154 667 1587 199
Fragments arranged by size:
1587 667 199 154
PasI CC'CwG_GG
Cuts at: 0 187 224 363 476 488 2412 2607
Size: 187 37 139 113 12 1924 195
Fragments arranged by size:
1924 195 187 139 113 37 12
PflMI CCAn_nnn'nTGG
Cuts at: 0 1329 1989 2607
Size: 1329 660 618
Fragments arranged by size:
1329 660 618
PfoI T'CCnGG_A
Cuts at: 0 452 657 1145 1612 1879 2607
Size: 452 205 488 467 267 728
Fragments arranged by size:
728 488 467 452 267 205
PpuMI rG'GwC_Cy
Cuts at: 0 162 228 249 456 796 1125 2216 2607
Size: 162 66 21 207 340 329 1091 391
Fragments arranged by size:
1091 391 340 329 207 162 66 21
PshAI GACnn'nnGTC
Cuts at: 0 430 2607
Size: 430 2177
PspOMI G'GGCC_C
Cuts at: 0 534 1242 2607
Size: 534 708 1365
Fragments arranged by size:
1365 708 534
PstI C_TGCA'G
Cuts at: 0 992 1066 1544 1673 1909 2081 2607
Size: 992 74 478 129 236 172 526
Fragments arranged by size:
992 526 478 236 172 129 74
PvuII CAG'CTG
Cuts at: 0 89 612 684 1499 1539 1545 2265 2607
Size: 89 523 72 815 40 6 720 342
Fragments arranged by size:
815 720 523 342 89 72 40 6
SacII CC_GC'GG
Cuts at: 0 264 2607
Size: 264 2343
SanDI GG'GwC_CC
Cuts at: 0 228 1125 2607
Size: 228 897 1482
Fragments arranged by size:
1482 897 228
SapI GCTCTTCn'nnn_
Cuts at: 0 1759 2607
Size: 1759 848
SfcI C'TryA_G
Cuts at: 0 988 1062 1119 1540 1669 1905 2077 2607
Size: 988 74 57 421 129 236 172 530
Fragments arranged by size:
988 530 421 236 172 129 74 57
SfoI GGC'GCC
Cuts at: 0 705 2607
Size: 705 1902
SmaI CCC'GGG
Cuts at: 0 247 1047 2229 2607
Size: 247 800 1182 378
Fragments arranged by size:
1182 800 378 247
SphI G_CATG'C
Cuts at: 0 2408 2607
Size: 2408 199
StuI AGG'CCT
Cuts at: 0 628 1745 2135 2518 2607
Size: 628 1117 390 383 89
Fragments arranged by size:
1117 628 390 383 89
StyI C'CwwG_G
Cuts at: 0 48 158 765 1443 2054 2607
Size: 48 110 607 678 611 553
Fragments arranged by size:
678 611 607 553 110 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1195 1864 1980 1990 2026 2607
Size: 1195 669 116 10 36 581
Fragments arranged by size:
1195 669 581 116 36 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 154 186 2487 2519 2607
Size: 154 32 2301 32 88
Fragments arranged by size:
2301 154 88 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 460 1743 2607
Size: 460 1283 864
Fragments arranged by size:
1283 864 460
XmaI C'CCGG_G
Cuts at: 0 245 1045 2227 2607
Size: 245 800 1182 380
Fragments arranged by size:
1182 800 380 245
ZraI GAC'GTC
Cuts at: 0 63 2607
Size: 63 2544
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BfrBI BglI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI ClaI CspCI DrdI
EaeI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I HincII KasI KpnI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PflMI PfoI
PpuMI PshAI PspOMI PstI PvuII SacII SanDI SapI
SfcI SfoI SmaI SphI StuI StyI TaqII TstI
XcmI XmaI ZraI
Enzymes that do not cut:
AclI AfeI AflII AloI ApaLI AscI AseI AsiSI
AvrII BaeI BciVI BclI BglII BlpI BmtI BpuEI
BsaAI BsaBI BsiEI BsiWI BsmI BspHI BsrGI BstBI
BstZ17I BtgZI DraI DraIII EagI EcoICRI EcoRI EcoRV
FalI FseI FspAI HindIII HpaI MfeI MluI MmeI
NdeI NheI NotI NruI PacI PciI PmeI PmlI
PpiI PsiI PspXI PsrI PvuI RsrII SacI SalI
SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI
SpeI SrfI SspI SwaI TatI Tth111I XbaI XhoI
XmnI