Restriction map for
pF1KB0442
(Linear) (Six-base) MAPSORT of: KIBB0442.seq Check: 2803 from: 1 to: 2706
KIBB0442.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2340 2706
Size: 2340 366
AccI GT'mk_AC
Cuts at: 0 1991 2706
Size: 1991 715
Acc65I G'GTAC_C
Cuts at: 0 2308 2706
Size: 2308 398
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 818 1176 1600 2090 2706
Size: 483 335 358 424 490 616
Fragments arranged by size:
616 490 483 424 358 335
AfeI AGC'GCT
Cuts at: 0 389 1738 2706
Size: 389 1349 968
Fragments arranged by size:
1349 968 389
AflIII A'CryG_T
Cuts at: 0 1642 1644 1668 2706
Size: 1642 2 24 1038
Fragments arranged by size:
1642 1038 24 2
AleI CACnn'nnGTG
Cuts at: 0 1513 2706
Size: 1513 1193
AlwNI CAG_nnn'CTG
Cuts at: 0 1752 1784 2706
Size: 1752 32 922
Fragments arranged by size:
1752 922 32
ApaI G_GGCC'C
Cuts at: 0 764 960 1293 2261 2706
Size: 764 196 333 968 445
Fragments arranged by size:
968 764 445 333 196
ApaLI G'TGCA_C
Cuts at: 0 1450 2706
Size: 1450 1256
ApoI r'AATT_y
Cuts at: 0 34 1590 2706
Size: 34 1556 1116
Fragments arranged by size:
1556 1116 34
AvaI C'yCGr_G
Cuts at: 0 331 653 819 917 924 1951 2706
Size: 331 322 166 98 7 1027 755
Fragments arranged by size:
1027 755 331 322 166 98 7
AvrII C'CTAG_G
Cuts at: 0 1362 2706
Size: 1362 1344
BanI G'GyrC_C
Cuts at: 0 247 1507 1716 2308 2706
Size: 247 1260 209 592 398
Fragments arranged by size:
1260 592 398 247 209
BanII G_rGCy'C
Cuts at: 0 152 637 764 931 960 1281 1293 2075
Size: 152 485 127 167 29 321 12 782
Cuts at: 2075 2261 2389 2559 2706
Size: 186 128 170 147
Fragments arranged by size:
782 485 321 186 170 167 152 147
128 127 29 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 991 2189 2706
Size: 991 1198 517
Fragments arranged by size:
1198 991 517
BbvCI CC'TCA_GC
Cuts at: 0 1255 2510 2706
Size: 1255 1255 196
Fragments arranged by size:
1255 1255 196
BciVI GTATCCnnnnn_n'
Cuts at: 0 2509 2706
Size: 2509 197
BfrBI ATG'CAT
Cuts at: 0 213 2706
Size: 213 2493
BglI GCCn_nnn'nGGC
Cuts at: 0 716 2706
Size: 716 1990
BlpI GC'TnA_GC
Cuts at: 0 1740 2706
Size: 1740 966
Bme1580I G_kGCm'C
Cuts at: 0 764 960 1142 1293 1454 1676 1721 2261
Size: 764 196 182 151 161 222 45 540
Cuts at: 2261 2325 2706
Size: 64 381
Fragments arranged by size:
764 540 381 222 196 182 161 151
64 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 1772 2706
Size: 1772 934
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 633 995 1059 2706
Size: 633 362 64 1647
Fragments arranged by size:
1647 633 362 64
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 711 1076 1270 2653 2706
Size: 412 299 365 194 1383 53
Fragments arranged by size:
1383 412 365 299 194 53
Bpu10I CC'TnA_GC
Cuts at: 0 1255 2510 2706
Size: 1255 1255 196
Fragments arranged by size:
1255 1255 196
BsaI GGTCTCn'nnnn_
Cuts at: 0 2654 2706
Size: 2654 52
BsaAI yAC'GTr
Cuts at: 0 1645 1671 2706
Size: 1645 26 1035
Fragments arranged by size:
1645 1035 26
BsaBI GATnn'nnATC
Cuts at: 0 1399 2495 2706
Size: 1399 1096 211
Fragments arranged by size:
1399 1096 211
BsaWI w'CCGG_w
Cuts at: 0 2079 2559 2706
Size: 2079 480 147
Fragments arranged by size:
2079 480 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 913 943 2706
Size: 913 30 1763
Fragments arranged by size:
1763 913 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 736 1048 1079 1090 1811 1906 1909
Size: 40 696 312 31 11 721 95 3
Cuts at: 1909 2083 2379 2432 2706
Size: 174 296 53 274
Fragments arranged by size:
721 696 312 296 274 174 95 53
40 31 11 3
BseYI C'CCAG_C
Cuts at: 0 75 282 304 313 340 768 843 1231
Size: 75 207 22 9 27 428 75 388
Cuts at: 1231 1733 2706
Size: 502 973
Fragments arranged by size:
973 502 428 388 207 75 75 27
22 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 2663 2706
Size: 152 2511 43
Fragments arranged by size:
2511 152 43
BsiEI CG_ry'CG
Cuts at: 0 2437 2706
Size: 2437 269
BsiHKAI G_wGCw'C
Cuts at: 0 756 1454 1732 2706
Size: 756 698 278 974
Fragments arranged by size:
974 756 698 278
BsmI GAATG_Cn'
Cuts at: 0 213 2706
Size: 213 2493
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1612 1701 2706
Size: 1612 89 1005
Fragments arranged by size:
1612 1005 89
Bsp1286I G_dGCh'C
Cuts at: 0 152 637 756 764 931 960 1142 1281
Size: 152 485 119 8 167 29 182 139
Cuts at: 1281 1293 1454 1676 1721 1732 2075 2261 2325
Size: 12 161 222 45 11 343 186 64
Cuts at: 2325 2389 2559 2706
Size: 64 170 147
Fragments arranged by size:
485 343 222 186 182 170 167 161
152 147 139 119 64 64 45 29
12 11 8
BspEI T'CCGG_A
Cuts at: 0 2559 2706
Size: 2559 147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1433 2340 2514 2706
Size: 1433 907 174 192
Fragments arranged by size:
1433 907 192 174
BsrDI GCAATG_nn'
Cuts at: 0 904 1056 1891 2706
Size: 904 152 835 815
Fragments arranged by size:
904 835 815 152
BssSI C'ACGA_G
Cuts at: 0 531 2706
Size: 531 2175
BstEII G'GTnAC_C
Cuts at: 0 1342 2706
Size: 1342 1364
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1154 1220 1518 2706
Size: 1154 66 298 1188
Fragments arranged by size:
1188 1154 298 66
BstYI r'GATC_y
Cuts at: 0 684 2706
Size: 684 2022
BtgI C'CryG_G
Cuts at: 0 670 778 858 1314 1478 2706
Size: 670 108 80 456 164 1228
Fragments arranged by size:
1228 670 456 164 108 80
BtsI GCAGTG_nn'
Cuts at: 0 1019 1142 1406 2706
Size: 1019 123 264 1300
Fragments arranged by size:
1300 1019 264 123
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 887 922 2706
Size: 887 35 1784
Fragments arranged by size:
1784 887 35
EaeI y'GGCC_r
Cuts at: 0 8 321 667 1475 1481 1553 1978 2434
Size: 8 313 346 808 6 72 425 456
Cuts at: 2434 2655 2706
Size: 221 51
Fragments arranged by size:
808 456 425 346 313 221 72 51
8 6
EagI C'GGCC_G
Cuts at: 0 2434 2706
Size: 2434 272
EarI CTCTTCn'nnn_
Cuts at: 0 869 2450 2687 2706
Size: 869 1581 237 19
Fragments arranged by size:
1581 869 237 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1008 2028 2408 2706
Size: 1008 1020 380 298
Fragments arranged by size:
1020 1008 380 298
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 483 711 818 1076 1176 1270 1600
Size: 412 71 228 107 258 100 94 330
Cuts at: 1600 2090 2653 2706
Size: 490 563 53
Fragments arranged by size:
563 490 412 330 258 228 107 100
94 71 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1094 2706
Size: 1094 1612
EcoO109I rG'GnC_Cy
Cuts at: 0 66 491 921 956 957 1196 1867 2156
Size: 66 425 430 35 1 239 671 289
Cuts at: 2156 2257 2258 2470 2706
Size: 101 1 212 236
Fragments arranged by size:
671 430 425 289 239 236 212 101
66 35 1 1
EcoRI G'AATT_C
Cuts at: 0 1590 2706
Size: 1590 1116
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1080 2706
Size: 1080 1626
HaeII r_GCGC'y
Cuts at: 0 167 391 810 1740 2706
Size: 167 224 419 930 966
Fragments arranged by size:
966 930 419 224 167
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 580 612 913 945 2594 2626 2706
Size: 580 32 301 32 1649 32 80
Fragments arranged by size:
1649 580 301 80 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1582 2706
Size: 1582 1124
KpnI G_GTAC'C
Cuts at: 0 2312 2706
Size: 2312 394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1499 1850 2706
Size: 1499 351 856
Fragments arranged by size:
1499 856 351
MscI TGG'CCA
Cuts at: 0 1477 2706
Size: 1477 1229
MslI CAynn'nnrTG
Cuts at: 0 1513 2134 2706
Size: 1513 621 572
Fragments arranged by size:
1513 621 572
MspA1I CmG'CkG
Cuts at: 0 14 75 642 780 967 1248 2594 2706
Size: 14 61 567 138 187 281 1346 112
Fragments arranged by size:
1346 567 281 187 138 112 61 14
NcoI C'CATG_G
Cuts at: 0 670 2706
Size: 670 2036
NsiI A_TGCA'T
Cuts at: 0 215 2706
Size: 215 2491
NspI r_CATG'y
Cuts at: 0 213 2235 2706
Size: 213 2022 471
Fragments arranged by size:
2022 471 213
PasI CC'CwG_GG
Cuts at: 0 1677 2400 2545 2706
Size: 1677 723 145 161
Fragments arranged by size:
1677 723 161 145
PflMI CCAn_nnn'nTGG
Cuts at: 0 511 2539 2706
Size: 511 2028 167
Fragments arranged by size:
2028 511 167
PfoI T'CCnGG_A
Cuts at: 0 1092 1102 2685 2706
Size: 1092 10 1583 21
Fragments arranged by size:
1583 1092 21 10
PmlI CAC'GTG
Cuts at: 0 1645 1671 2706
Size: 1645 26 1035
Fragments arranged by size:
1645 1035 26
PpuMI rG'GwC_Cy
Cuts at: 0 921 1196 1867 2706
Size: 921 275 671 839
Fragments arranged by size:
921 839 671 275
PspOMI G'GGCC_C
Cuts at: 0 760 956 1289 2257 2706
Size: 760 196 333 968 449
Fragments arranged by size:
968 760 449 333 196
PstI C_TGCA'G
Cuts at: 0 1413 1919 2337 2706
Size: 1413 506 418 369
Fragments arranged by size:
1413 506 418 369
PvuII CAG'CTG
Cuts at: 0 14 967 1248 2706
Size: 14 953 281 1458
Fragments arranged by size:
1458 953 281 14
SacII CC_GC'GG
Cuts at: 0 781 2706
Size: 781 1925
SanDI GG'GwC_CC
Cuts at: 0 921 2706
Size: 921 1785
SapI GCTCTTCn'nnn_
Cuts at: 0 2687 2706
Size: 2687 19
SfcI C'TryA_G
Cuts at: 0 462 1368 1390 1409 1770 1915 2271 2333
Size: 462 906 22 19 361 145 356 62
Cuts at: 2333 2586 2706
Size: 253 120
Fragments arranged by size:
906 462 361 356 253 145 120 62
22 19
SmaI CCC'GGG
Cuts at: 0 655 919 926 2706
Size: 655 264 7 1780
Fragments arranged by size:
1780 655 264 7
SmlI C'TyrA_G
Cuts at: 0 331 2706
Size: 331 2375
SphI G_CATG'C
Cuts at: 0 213 2235 2706
Size: 213 2022 471
Fragments arranged by size:
2022 471 213
StuI AGG'CCT
Cuts at: 0 1768 2706
Size: 1768 938
StyI C'CwwG_G
Cuts at: 0 140 670 1064 1160 1362 1511 1844 2706
Size: 140 530 394 96 202 149 333 862
Fragments arranged by size:
862 530 394 333 202 149 140 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1016 2166 2706
Size: 1016 1150 540
Fragments arranged by size:
1150 1016 540
TatI w'GTAC_w
Cuts at: 0 1701 1955 2706
Size: 1701 254 751
Fragments arranged by size:
1701 751 254
Tth111I GACn'n_nGTC
Cuts at: 0 2608 2706
Size: 2608 98
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 734 2706
Size: 734 1972
XhoI C'TCGA_G
Cuts at: 0 331 2706
Size: 331 2375
XmaI C'CCGG_G
Cuts at: 0 653 917 924 2706
Size: 653 264 7 1782
Fragments arranged by size:
1782 653 264 7
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AleI AlwNI
ApaI ApaLI ApoI AvaI AvrII BanI BanII BbsI
BbvCI BciVI BfrBI BglI BlpI Bme1580I BmrI BplI
BpmI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrDI BssSI BstEII BstXI BstYI BtgI BtsI
CspCI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HindIII KpnI MmeI MscI
MslI MspA1I NcoI NsiI NspI PasI PflMI PfoI
PmlI PpuMI PspOMI PstI PvuII SacII SanDI SapI
SfcI SmaI SmlI SphI StuI StyI TaqII TatI
Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AlfI AloI AscI
AseI AsiSI BaeI BamHI BbeI BcgI BclI BglII
BmgBI BmtI BpuEI BsaHI BsiWI BspHI BsrBI BsrFI
BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI
DraI DraIII DrdI EcoICRI EcoRV FseI FspI FspAI
HincII HpaI KasI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PciI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI TstI XbaI XmnI ZraI