Restriction map for
pF1KB0468
(Linear) (Six-base) MAPSORT of: KIBB0468.seq Check: 7576 from: 1 to: 2952
KIBB0468.orf
With 182 enzymes: SgfI *
June 14, 2010 14:44 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 205 1005 1814 1963 2554 2875 2952
Size: 50 155 800 809 149 591 321 77
Fragments arranged by size:
809 800 591 321 155 149 77 50
AfeI AGC'GCT
Cuts at: 0 2198 2952
Size: 2198 754
AhdI GACnn_n'nnGTC
Cuts at: 0 1698 2952
Size: 1698 1254
AleI CACnn'nnGTG
Cuts at: 0 836 2952
Size: 836 2116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 767 2543 2952
Size: 767 1776 409
Fragments arranged by size:
1776 767 409
AlwNI CAG_nnn'CTG
Cuts at: 0 298 2952
Size: 298 2654
ApaLI G'TGCA_C
Cuts at: 0 673 2952
Size: 673 2279
ApoI r'AATT_y
Cuts at: 0 424 1251 1412 1548 2004 2818 2952
Size: 424 827 161 136 456 814 134
Fragments arranged by size:
827 814 456 424 161 136 134
BamHI G'GATC_C
Cuts at: 0 1051 2952
Size: 1051 1901
BanI G'GyrC_C
Cuts at: 0 1039 1312 2952
Size: 1039 273 1640
Fragments arranged by size:
1640 1039 273
BanII G_rGCy'C
Cuts at: 0 511 775 2952
Size: 511 264 2177
Fragments arranged by size:
2177 511 264
BbsI GAAGACnn'nnnn_
Cuts at: 0 1981 2952
Size: 1981 971
BbvCI CC'TCA_GC
Cuts at: 0 2174 2952
Size: 2174 778
BciVI GTATCCnnnnn_n'
Cuts at: 0 1730 2952
Size: 1730 1222
BfrBI ATG'CAT
Cuts at: 0 2520 2793 2952
Size: 2520 273 159
Fragments arranged by size:
2520 273 159
BlpI GC'TnA_GC
Cuts at: 0 2313 2952
Size: 2313 639
Bme1580I G_kGCm'C
Cuts at: 0 677 1433 2952
Size: 677 756 1519
Fragments arranged by size:
1519 756 677
BmrI ACTGGGnnnn_n'
Cuts at: 0 1073 1462 2952
Size: 1073 389 1490
Fragments arranged by size:
1490 1073 389
BmtI G_CTAG'C
Cuts at: 0 764 2952
Size: 764 2188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 720 833 840 2466 2952
Size: 720 113 7 1626 486
Fragments arranged by size:
1626 720 486 113 7
Bpu10I CC'TnA_GC
Cuts at: 0 2174 2952
Size: 2174 778
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 501 768 900 1091 1571 2404 2460
Size: 164 337 267 132 191 480 833 56
Cuts at: 2460 2952
Size: 492
Fragments arranged by size:
833 492 480 337 267 191 164 132
56
BsaI GGTCTCn'nnnn_
Cuts at: 0 1613 2952
Size: 1613 1339
BsaBI GATnn'nnATC
Cuts at: 0 1718 2952
Size: 1718 1234
BsaHI Gr'CG_yC
Cuts at: 0 524 2952
Size: 524 2428
BsaWI w'CCGG_w
Cuts at: 0 154 1722 2945 2952
Size: 154 1568 1223 7
Fragments arranged by size:
1568 1223 154 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 689 767 2071 2952
Size: 689 78 1304 881
Fragments arranged by size:
1304 881 689 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 737 2952
Size: 737 2215
BsiEI CG_ry'CG
Cuts at: 0 1612 2952
Size: 1612 1340
BsiHKAI G_wGCw'C
Cuts at: 0 511 677 925 1107 1587 2016 2952
Size: 511 166 248 182 480 429 936
Fragments arranged by size:
936 511 480 429 248 182 166
BsmI GAATG_Cn'
Cuts at: 0 1299 2952
Size: 1299 1653
Bsp1286I G_dGCh'C
Cuts at: 0 511 677 775 925 1107 1433 1587 2016
Size: 511 166 98 150 182 326 154 429
Cuts at: 2016 2952
Size: 936
Fragments arranged by size:
936 511 429 326 182 166 154 150
98
BspHI T'CATG_A
Cuts at: 0 561 2612 2952
Size: 561 2051 340
Fragments arranged by size:
2051 561 340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2819 2952
Size: 2819 133
BsrBI CCG'CTC
Cuts at: 0 2934 2952
Size: 2934 18
BsrDI GCAATG_nn'
Cuts at: 0 771 1060 2879 2952
Size: 771 289 1819 73
Fragments arranged by size:
1819 771 289 73
BssHII G'CGCG_C
Cuts at: 0 531 2952
Size: 531 2421
BssSI C'ACGA_G
Cuts at: 0 2053 2192 2952
Size: 2053 139 760
Fragments arranged by size:
2053 760 139
BstBI TT'CG_AA
Cuts at: 0 940 2952
Size: 940 2012
BstXI CCAn_nnnn'nTGG
Cuts at: 0 987 2781 2952
Size: 987 1794 171
Fragments arranged by size:
1794 987 171
BstYI r'GATC_y
Cuts at: 0 245 880 955 991 1051 1783 2952
Size: 245 635 75 36 60 732 1169
Fragments arranged by size:
1169 732 635 245 75 60 36
BtgI C'CryG_G
Cuts at: 0 311 2952
Size: 311 2641
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2115 2952
Size: 2115 837
BtsI GCAGTG_nn'
Cuts at: 0 831 843 1028 1895 2952
Size: 831 12 185 867 1057
Fragments arranged by size:
1057 867 831 185 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2861 2896 2952
Size: 2861 35 56
Fragments arranged by size:
2861 56 35
DraI TTT'AAA
Cuts at: 0 1323 2370 2952
Size: 1323 1047 582
Fragments arranged by size:
1323 1047 582
DrdI GACnn_nn'nnGTC
Cuts at: 0 56 2952
Size: 56 2896
EaeI y'GGCC_r
Cuts at: 0 441 1167 2952
Size: 441 726 1785
Fragments arranged by size:
1785 726 441
EarI CTCTTCn'nnn_
Cuts at: 0 2504 2952
Size: 2504 448
EcoICRI GAG'CTC
Cuts at: 0 509 2952
Size: 509 2443
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 205 720 833 840 1005 1814 1963
Size: 50 155 515 113 7 165 809 149
Cuts at: 1963 2466 2554 2875 2952
Size: 503 88 321 77
Fragments arranged by size:
809 515 503 321 165 155 149 113
88 77 50 7
EcoO109I rG'GnC_Cy
Cuts at: 0 94 1113 2952
Size: 94 1019 1839
Fragments arranged by size:
1839 1019 94
EcoRI G'AATT_C
Cuts at: 0 1548 2818 2952
Size: 1548 1270 134
Fragments arranged by size:
1548 1270 134
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1778 2952
Size: 1778 1174
HaeII r_GCGC'y
Cuts at: 0 2200 2952
Size: 2200 752
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 262 294 1569 1601 1684 1716 2573 2605
Size: 262 32 1275 32 83 32 857 32
Cuts at: 2605 2952
Size: 347
Fragments arranged by size:
1275 857 347 262 83 32 32 32
32
HincII GTy'rAC
Cuts at: 0 138 2157 2952
Size: 138 2019 795
Fragments arranged by size:
2019 795 138
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1357 1656 2952
Size: 1357 299 1296
Fragments arranged by size:
1357 1296 299
MscI TGG'CCA
Cuts at: 0 443 1169 2952
Size: 443 726 1783
Fragments arranged by size:
1783 726 443
MslI CAynn'nnrTG
Cuts at: 0 836 2611 2952
Size: 836 1775 341
Fragments arranged by size:
1775 836 341
MspA1I CmG'CkG
Cuts at: 0 417 1460 1865 2901 2952
Size: 417 1043 405 1036 51
Fragments arranged by size:
1043 1036 417 405 51
NcoI C'CATG_G
Cuts at: 0 311 2952
Size: 311 2641
NheI G'CTAG_C
Cuts at: 0 760 2952
Size: 760 2192
NsiI A_TGCA'T
Cuts at: 0 2522 2795 2952
Size: 2522 273 157
Fragments arranged by size:
2522 273 157
NspI r_CATG'y
Cuts at: 0 710 760 2952
Size: 710 50 2192
Fragments arranged by size:
2192 710 50
PasI CC'CwG_GG
Cuts at: 0 90 690 2952
Size: 90 600 2262
Fragments arranged by size:
2262 600 90
PflMI CCAn_nnn'nTGG
Cuts at: 0 2650 2952
Size: 2650 302
PfoI T'CCnGG_A
Cuts at: 0 2361 2952
Size: 2361 591
PpuMI rG'GwC_Cy
Cuts at: 0 94 1113 2952
Size: 94 1019 1839
Fragments arranged by size:
1839 1019 94
PstI C_TGCA'G
Cuts at: 0 838 2588 2952
Size: 838 1750 364
Fragments arranged by size:
1750 838 364
PvuI CG_AT'CG
Cuts at: 0 1612 2952
Size: 1612 1340
PvuII CAG'CTG
Cuts at: 0 417 1865 2901 2952
Size: 417 1448 1036 51
Fragments arranged by size:
1448 1036 417 51
SacI G_AGCT'C
Cuts at: 0 511 2952
Size: 511 2441
ScaI AGT'ACT
Cuts at: 0 177 984 2952
Size: 177 807 1968
Fragments arranged by size:
1968 807 177
SexAI A'CCwGG_T
Cuts at: 0 2798 2952
Size: 2798 154
SfcI C'TryA_G
Cuts at: 0 98 299 834 999 1572 2584 2952
Size: 98 201 535 165 573 1012 368
Fragments arranged by size:
1012 573 535 368 201 165 98
SmlI C'TyrA_G
Cuts at: 0 179 480 783 915 1106 1586 2383 2439
Size: 179 301 303 132 191 480 797 56
Cuts at: 2439 2952
Size: 513
Fragments arranged by size:
797 513 480 303 301 191 179 132
56
SspI AAT'ATT
Cuts at: 0 906 1179 2952
Size: 906 273 1773
Fragments arranged by size:
1773 906 273
StyI C'CwwG_G
Cuts at: 0 311 2906 2952
Size: 311 2595 46
Fragments arranged by size:
2595 311 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 124 703 2142 2952
Size: 124 579 1439 810
Fragments arranged by size:
1439 810 579 124
TatI w'GTAC_w
Cuts at: 0 175 982 1057 1187 2952
Size: 175 807 75 130 1765
Fragments arranged by size:
1765 807 175 130 75
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 37 69 1412 1444 1454 1486 1627 1659
Size: 37 32 1343 32 10 32 141 32
Cuts at: 1659 2952
Size: 1293
Fragments arranged by size:
1343 1293 141 37 32 32 32 32
10
Tth111I GACn'n_nGTC
Cuts at: 0 2497 2952
Size: 2497 455
XbaI T'CTAG_A
Cuts at: 0 36 1702 2952
Size: 36 1666 1250
Fragments arranged by size:
1666 1250 36
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1576 2796 2952
Size: 1576 1220 156
Fragments arranged by size:
1576 1220 156
XmnI GAAnn'nnTTC
Cuts at: 0 1736 1773 2952
Size: 1736 37 1179
Fragments arranged by size:
1736 1179 37
Enzymes that do cut:
AcuI AfeI AhdI AleI AlfI AlwNI ApaLI ApoI
BamHI BanI BanII BbsI BbvCI BciVI BfrBI BlpI
Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BssHII BssSI BstBI BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
HincII MmeI MscI MslI MspA1I NcoI NheI NsiI
NspI PasI PflMI PfoI PpuMI PstI PvuI PvuII
SacI ScaI SexAI SfcI SmlI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BcgI BclI BglI BglII BmgBI BplI BsaAI
BsaXI BseYI BsiWI BsmBI BspEI BsrFI BsrGI BstAPI
BstEII BstZ17I Bsu36I ClaI DraIII EagI EciI EcoNI
EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NotI NruI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI RsrII SacII SalI SanDI SapI SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI XhoI XmaI ZraI