Restriction map for
pF1KB0470
(Linear) (Six-base) MAPSORT of: KIBB0470.seq Check: 1591 from: 1 to: 2973
KIBB0470.orf
With 182 enzymes: SgfI *
September 24, 2008 10:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 279 1596 2973
Size: 279 1317 1377
Fragments arranged by size:
1377 1317 279
AccI GT'mk_AC
Cuts at: 0 2228 2973
Size: 2228 745
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 1355 1723 2942 2973
Size: 613 742 368 1219 31
Fragments arranged by size:
1219 742 613 368 31
AfeI AGC'GCT
Cuts at: 0 238 2973
Size: 238 2735
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 532 756 2973
Size: 532 224 2217
Fragments arranged by size:
2217 532 224
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 417 449 2973
Size: 417 32 2524
Fragments arranged by size:
2524 417 32
AlwNI CAG_nnn'CTG
Cuts at: 0 83 198 580 813 1482 1612 2595 2817
Size: 83 115 382 233 669 130 983 222
Cuts at: 2817 2860 2973
Size: 43 113
Fragments arranged by size:
983 669 382 233 222 130 115 113
83 43
ApaI G_GGCC'C
Cuts at: 0 943 1103 1234 2973
Size: 943 160 131 1739
Fragments arranged by size:
1739 943 160 131
ApaLI G'TGCA_C
Cuts at: 0 2239 2785 2973
Size: 2239 546 188
Fragments arranged by size:
2239 546 188
AvaI C'yCGr_G
Cuts at: 0 36 280 944 1003 1090 1499 1562 1625
Size: 36 244 664 59 87 409 63 63
Cuts at: 1625 2331 2474 2973
Size: 706 143 499
Fragments arranged by size:
706 664 499 409 244 143 87 63
63 59 36
BamHI G'GATC_C
Cuts at: 0 2427 2709 2973
Size: 2427 282 264
Fragments arranged by size:
2427 282 264
BanI G'GyrC_C
Cuts at: 0 40 1015 1547 1736 1885 2254 2841 2973
Size: 40 975 532 189 149 369 587 132
Fragments arranged by size:
975 587 532 369 189 149 132 40
BanII G_rGCy'C
Cuts at: 0 37 155 167 251 473 587 790 943
Size: 37 118 12 84 222 114 203 153
Cuts at: 943 967 1004 1103 1196 1234 1569 1796 1937
Size: 24 37 99 93 38 335 227 141
Cuts at: 1937 2973
Size: 1036
Fragments arranged by size:
1036 335 227 222 203 153 141 118
114 99 93 84 38 37 37 24
12
BbsI GAAGACnn'nnnn_
Cuts at: 0 1343 1565 2973
Size: 1343 222 1408
Fragments arranged by size:
1408 1343 222
BbvCI CC'TCA_GC
Cuts at: 0 104 2525 2973
Size: 104 2421 448
Fragments arranged by size:
2421 448 104
BciVI GTATCCnnnnn_n'
Cuts at: 0 2572 2973
Size: 2572 401
BclI T'GATC_A
Cuts at: 0 1277 2973
Size: 1277 1696
BglI GCCn_nnn'nGGC
Cuts at: 0 62 255 1621 2035 2674 2683 2973
Size: 62 193 1366 414 639 9 290
Fragments arranged by size:
1366 639 414 290 193 62 9
BlpI GC'TnA_GC
Cuts at: 0 505 1897 2973
Size: 505 1392 1076
Fragments arranged by size:
1392 1076 505
Bme1580I G_kGCm'C
Cuts at: 0 45 943 1018 1091 1103 1234 1550 2243
Size: 45 898 75 73 12 131 316 693
Cuts at: 2243 2789 2973
Size: 546 184
Fragments arranged by size:
898 693 546 316 184 131 75 73
45 12
BmgBI CAC'GTC
Cuts at: 0 1269 2238 2973
Size: 1269 969 735
Fragments arranged by size:
1269 969 735
BmrI ACTGGGnnnn_n'
Cuts at: 0 139 533 1116 1199 2241 2276 2554 2808
Size: 139 394 583 83 1042 35 278 254
Cuts at: 2808 2973
Size: 165
Fragments arranged by size:
1042 583 394 278 254 165 139 83
35
BmtI G_CTAG'C
Cuts at: 0 385 2973
Size: 385 2588
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1164 1199 2973
Size: 1164 35 1774
Fragments arranged by size:
1774 1164 35
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 496 643 1270 1363 1408 1426 1620
Size: 487 9 147 627 93 45 18 194
Cuts at: 1620 2423 2471 2788 2973
Size: 803 48 317 185
Fragments arranged by size:
803 627 487 317 194 185 147 93
48 45 18 9
Bpu10I CC'TnA_GC
Cuts at: 0 104 2525 2973
Size: 104 2421 448
Fragments arranged by size:
2421 448 104
BsaI GGTCTCn'nnnn_
Cuts at: 0 685 1639 2973
Size: 685 954 1334
Fragments arranged by size:
1334 954 685
BsaAI yAC'GTr
Cuts at: 0 1872 2973
Size: 1872 1101
BsaBI GATnn'nnATC
Cuts at: 0 2467 2973
Size: 2467 506
BsaHI Gr'CG_yC
Cuts at: 0 2666 2896 2973
Size: 2666 230 77
Fragments arranged by size:
2666 230 77
BsaWI w'CCGG_w
Cuts at: 0 1796 2973
Size: 1796 1177
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1699 1729 1791 1821 2603 2633 2973
Size: 1699 30 62 30 782 30 340
Fragments arranged by size:
1699 782 340 62 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 696 1036 1427 1867 2965 2973
Size: 696 340 391 440 1098 8
Fragments arranged by size:
1098 696 440 391 340 8
BseYI C'CCAG_C
Cuts at: 0 191 529 667 1009 1401 1486 1578 2033
Size: 191 338 138 342 392 85 92 455
Cuts at: 2033 2258 2973
Size: 225 715
Fragments arranged by size:
715 455 392 342 338 225 191 138
92 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 184 2973
Size: 107 77 2789
Fragments arranged by size:
2789 107 77
BsiEI CG_ry'CG
Cuts at: 0 1755 2973
Size: 1755 1218
BsiHKAI G_wGCw'C
Cuts at: 0 557 767 1394 1717 1748 1877 2243 2291
Size: 557 210 627 323 31 129 366 48
Cuts at: 2291 2789 2973
Size: 498 184
Fragments arranged by size:
627 557 498 366 323 210 184 129
48 31
BsmI GAATG_Cn'
Cuts at: 0 449 2973
Size: 449 2524
BsmBI CGTCTCn'nnnn_
Cuts at: 0 472 2973
Size: 472 2501
Bsp1286I G_dGCh'C
Cuts at: 0 37 45 155 167 251 473 557 587
Size: 37 8 110 12 84 222 84 30
Cuts at: 587 767 790 943 967 1004 1018 1091 1103
Size: 180 23 153 24 37 14 73 12
Cuts at: 1103 1196 1234 1394 1550 1569 1717 1748 1796
Size: 93 38 160 156 19 148 31 48
Cuts at: 1796 1877 1937 2243 2291 2789 2973
Size: 81 60 306 48 498 184
Fragments arranged by size:
498 306 222 184 180 160 156 153
148 110 93 84 84 81 73 60
48 48 38 37 37 31 30 24
23 19 14 12 12 8
BspEI T'CCGG_A
Cuts at: 0 1796 2973
Size: 1796 1177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 131 279 1596 2973
Size: 131 148 1317 1377
Fragments arranged by size:
1377 1317 148 131
BsrBI CCG'CTC
Cuts at: 0 497 627 1143 1216 1435 1788 1866 1896
Size: 497 130 516 73 219 353 78 30
Cuts at: 1896 2762 2973
Size: 866 211
Fragments arranged by size:
866 516 497 353 219 211 130 78
73 30
BsrDI GCAATG_nn'
Cuts at: 0 169 2973
Size: 169 2804
BsrFI r'CCGG_y
Cuts at: 0 1550 1750 2183 2973
Size: 1550 200 433 790
Fragments arranged by size:
1550 790 433 200
BssSI C'ACGA_G
Cuts at: 0 2078 2973
Size: 2078 895
BstEII G'GTnAC_C
Cuts at: 0 2530 2973
Size: 2530 443
BstXI CCAn_nnnn'nTGG
Cuts at: 0 174 366 2837 2973
Size: 174 192 2471 136
Fragments arranged by size:
2471 192 174 136
BstYI r'GATC_y
Cuts at: 0 2216 2427 2709 2973
Size: 2216 211 282 264
Fragments arranged by size:
2216 282 264 211
Bsu36I CC'TnA_GG
Cuts at: 0 1059 1418 1454 2973
Size: 1059 359 36 1519
Fragments arranged by size:
1519 1059 359 36
BtgI C'CryG_G
Cuts at: 0 116 263 990 1407 2144 2244 2420 2973
Size: 116 147 727 417 737 100 176 553
Fragments arranged by size:
737 727 553 417 176 147 116 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2160 2290 2454 2973
Size: 2160 130 164 519
Fragments arranged by size:
2160 519 164 130
BtsI GCAGTG_nn'
Cuts at: 0 640 2052 2624 2888 2973
Size: 640 1412 572 264 85
Fragments arranged by size:
1412 640 572 264 85
DraIII CAC_nnn'GTG
Cuts at: 0 2548 2973
Size: 2548 425
EaeI y'GGCC_r
Cuts at: 0 1168 1752 2171 2640 2973
Size: 1168 584 419 469 333
Fragments arranged by size:
1168 584 469 419 333
EagI C'GGCC_G
Cuts at: 0 1752 2973
Size: 1752 1221
EarI CTCTTCn'nnn_
Cuts at: 0 217 985 1835 2696 2973
Size: 217 768 850 861 277
Fragments arranged by size:
861 850 768 277 217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 496 613 643 1270 1355 1363 1408
Size: 487 9 117 30 627 85 8 45
Cuts at: 1408 1426 1620 1723 2423 2471 2788 2942 2973
Size: 18 194 103 700 48 317 154 31
Fragments arranged by size:
700 627 487 317 194 154 117 103
85 48 45 31 30 18 9 8
EcoO109I rG'GnC_Cy
Cuts at: 0 23 80 100 295 923 940 1100 1231
Size: 23 57 20 195 628 17 160 131
Cuts at: 1231 1429 1658 2930 2973
Size: 198 229 1272 43
Fragments arranged by size:
1272 628 229 198 195 160 131 57
43 23 20 17
FspI TGC'GCA
Cuts at: 0 568 2973
Size: 568 2405
HaeII r_GCGC'y
Cuts at: 0 17 198 240 799 2817 2973
Size: 17 181 42 559 2018 156
Fragments arranged by size:
2018 559 181 156 42 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 621 653 1834 1866 2917 2949 2973
Size: 621 32 1181 32 1051 32 24
Fragments arranged by size:
1181 1051 621 32 32 32 24
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 2973
Size: 955 2018
MscI TGG'CCA
Cuts at: 0 1170 2173 2642 2973
Size: 1170 1003 469 331
Fragments arranged by size:
1170 1003 469 331
MslI CAynn'nnrTG
Cuts at: 0 274 738 2309 2973
Size: 274 464 1571 664
Fragments arranged by size:
1571 664 464 274
MspA1I CmG'CkG
Cuts at: 0 138 810 1077 1947 2033 2041 2190 2369
Size: 138 672 267 870 86 8 149 179
Cuts at: 2369 2943 2973
Size: 574 30
Fragments arranged by size:
870 672 574 267 179 149 138 86
30 8
NaeI GCC'GGC
Cuts at: 0 1752 2185 2973
Size: 1752 433 788
Fragments arranged by size:
1752 788 433
NcoI C'CATG_G
Cuts at: 0 116 263 990 1407 2144 2244 2420 2973
Size: 116 147 727 417 737 100 176 553
Fragments arranged by size:
737 727 553 417 176 147 116 100
NgoMIV G'CCGG_C
Cuts at: 0 1750 2183 2973
Size: 1750 433 790
Fragments arranged by size:
1750 790 433
NheI G'CTAG_C
Cuts at: 0 381 2973
Size: 381 2592
NspI r_CATG'y
Cuts at: 0 2811 2973
Size: 2811 162
PasI CC'CwG_GG
Cuts at: 0 1539 2973
Size: 1539 1434
PflMI CCAn_nnn'nTGG
Cuts at: 0 996 1374 2548 2973
Size: 996 378 1174 425
Fragments arranged by size:
1174 996 425 378
PfoI T'CCnGG_A
Cuts at: 0 1049 2973
Size: 1049 1924
PmlI CAC'GTG
Cuts at: 0 1872 2973
Size: 1872 1101
PpuMI rG'GwC_Cy
Cuts at: 0 23 295 923 1658 2973
Size: 23 272 628 735 1315
Fragments arranged by size:
1315 735 628 272 23
PspOMI G'GGCC_C
Cuts at: 0 939 1099 1230 2973
Size: 939 160 131 1743
Fragments arranged by size:
1743 939 160 131
PstI C_TGCA'G
Cuts at: 0 55 185 230 824 1037 1784 1850 2973
Size: 55 130 45 594 213 747 66 1123
Fragments arranged by size:
1123 747 594 213 130 66 55 45
PvuII CAG'CTG
Cuts at: 0 2190 2943 2973
Size: 2190 753 30
Fragments arranged by size:
2190 753 30
SanDI GG'GwC_CC
Cuts at: 0 23 295 2973
Size: 23 272 2678
Fragments arranged by size:
2678 272 23
SapI GCTCTTCn'nnn_
Cuts at: 0 1835 2696 2973
Size: 1835 861 277
Fragments arranged by size:
1835 861 277
SfcI C'TryA_G
Cuts at: 0 51 181 226 353 456 820 1033 1780
Size: 51 130 45 127 103 364 213 747
Cuts at: 1780 1846 2973
Size: 66 1127
Fragments arranged by size:
1127 747 364 213 130 127 103 66
51 45
SmaI CCC'GGG
Cuts at: 0 38 1092 1501 1564 2333 2973
Size: 38 1054 409 63 769 640
Fragments arranged by size:
1054 769 640 409 63 38
SphI G_CATG'C
Cuts at: 0 2811 2973
Size: 2811 162
SrfI GCCC'GGGC
Cuts at: 0 1092 2973
Size: 1092 1881
StuI AGG'CCT
Cuts at: 0 224 1160 1831 2677 2973
Size: 224 936 671 846 296
Fragments arranged by size:
936 846 671 296 224
StyI C'CwwG_G
Cuts at: 0 116 263 990 1407 2144 2244 2420 2630
Size: 116 147 727 417 737 100 176 210
Cuts at: 2630 2973
Size: 343
Fragments arranged by size:
737 727 417 343 210 176 147 116
100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1683 1842 2272 2536 2829 2973
Size: 1683 159 430 264 293 144
Fragments arranged by size:
1683 430 293 264 159 144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1699 1731 1791 1823 2603 2635 2973
Size: 1699 32 60 32 780 32 338
Fragments arranged by size:
1699 780 338 60 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 858 1693 2494 2973
Size: 858 835 801 479
Fragments arranged by size:
858 835 801 479
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 618 780 1047 2763 2973
Size: 618 162 267 1716 210
Fragments arranged by size:
1716 618 267 210 162
XmaI C'CCGG_G
Cuts at: 0 36 1090 1499 1562 2331 2973
Size: 36 1054 409 63 769 642
Fragments arranged by size:
1054 769 642 409 63 36
XmnI GAAnn'nnTTC
Cuts at: 0 2095 2973
Size: 2095 878
Enzymes that do cut:
AarI AccI AcuI AfeI AlfI AloI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbsI BbvCI BciVI
BclI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BssSI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI
EarI Eco57MI EcoO109I FspI HaeII Hin4I MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NheI NspI PasI
PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SanDI
SapI SfcI SmaI SphI SrfI StuI StyI TaqII
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BcgI
BfrBI BglII BpuEI BsiWI BspHI BsrGI BssHII BstAPI
BstBI BstZ17I ClaI CspCI DraI DrdI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI NarI NdeI NotI
NruI NsiI PacI PciI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SspI SwaI TatI XbaI XhoI ZraI