Restriction map for
pF1KB0473
(Linear) (Six-base) MAPSORT of: KIBB0473.seq Check: 7023 from: 1 to: 1497
KIBB0473.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 786 1497
Size: 786 711
AccI GT'mk_AC
Cuts at: 0 212 1201 1497
Size: 212 989 296
Fragments arranged by size:
989 296 212
Acc65I G'GTAC_C
Cuts at: 0 856 1497
Size: 856 641
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 1472 1497
Size: 55 1417 25
Fragments arranged by size:
1417 55 25
AflIII A'CryG_T
Cuts at: 0 296 1497
Size: 296 1201
AleI CACnn'nnGTG
Cuts at: 0 964 1497
Size: 964 533
AlwNI CAG_nnn'CTG
Cuts at: 0 799 808 909 1182 1497
Size: 799 9 101 273 315
Fragments arranged by size:
799 315 273 101 9
ApaLI G'TGCA_C
Cuts at: 0 517 1497
Size: 517 980
AvaI C'yCGr_G
Cuts at: 0 52 593 1096 1146 1497
Size: 52 541 503 50 351
Fragments arranged by size:
541 503 351 52 50
BanI G'GyrC_C
Cuts at: 0 856 945 1497
Size: 856 89 552
Fragments arranged by size:
856 552 89
BanII G_rGCy'C
Cuts at: 0 223 1064 1497
Size: 223 841 433
Fragments arranged by size:
841 433 223
BbsI GAAGACnn'nnnn_
Cuts at: 0 665 1497
Size: 665 832
BbvCI CC'TCA_GC
Cuts at: 0 476 1497
Size: 476 1021
BglI GCCn_nnn'nGGC
Cuts at: 0 1408 1497
Size: 1408 89
BglII A'GATC_T
Cuts at: 0 1361 1497
Size: 1361 136
BlpI GC'TnA_GC
Cuts at: 0 176 1120 1497
Size: 176 944 377
Fragments arranged by size:
944 377 176
Bme1580I G_kGCm'C
Cuts at: 0 521 868 916 1497
Size: 521 347 48 581
Fragments arranged by size:
581 521 347 48
BmrI ACTGGGnnnn_n'
Cuts at: 0 645 1497
Size: 645 852
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 785 971 1078 1497
Size: 785 186 107 419
Fragments arranged by size:
785 419 186 107
Bpu10I CC'TnA_GC
Cuts at: 0 476 824 1497
Size: 476 348 673
Fragments arranged by size:
673 476 348
BsaI GGTCTCn'nnnn_
Cuts at: 0 1057 1497
Size: 1057 440
BsaAI yAC'GTr
Cuts at: 0 216 507 1497
Size: 216 291 990
Fragments arranged by size:
990 291 216
BsaHI Gr'CG_yC
Cuts at: 0 49 1497
Size: 49 1448
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 833 863 1497
Size: 833 30 634
Fragments arranged by size:
833 634 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 493 1099 1288 1348 1351 1497
Size: 493 606 189 60 3 146
Fragments arranged by size:
606 493 189 146 60 3
BseYI C'CCAG_C
Cuts at: 0 30 237 402 433 452 852 885 902
Size: 30 207 165 31 19 400 33 17
Cuts at: 902 972 1047 1497
Size: 70 75 450
Fragments arranged by size:
450 400 207 165 75 70 33 31
30 19 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 567 1497
Size: 350 217 930
Fragments arranged by size:
930 350 217
BsiHKAI G_wGCw'C
Cuts at: 0 19 521 1136 1497
Size: 19 502 615 361
Fragments arranged by size:
615 502 361 19
Bsp1286I G_dGCh'C
Cuts at: 0 19 223 521 868 916 1064 1136 1497
Size: 19 204 298 347 48 148 72 361
Fragments arranged by size:
361 347 298 204 148 72 48 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 170 786 1497
Size: 170 616 711
Fragments arranged by size:
711 616 170
BsrBI CCG'CTC
Cuts at: 0 1107 1497
Size: 1107 390
BstAPI GCAn_nnn'nTGC
Cuts at: 0 799 808 1182 1497
Size: 799 9 374 315
Fragments arranged by size:
799 374 315 9
BstEII G'GTnAC_C
Cuts at: 0 993 1497
Size: 993 504
BstXI CCAn_nnnn'nTGG
Cuts at: 0 949 1497
Size: 949 548
BstYI r'GATC_y
Cuts at: 0 1361 1497
Size: 1361 136
BtgI C'CryG_G
Cuts at: 0 92 1497
Size: 92 1405
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 117 1497
Size: 117 1380
BtsI GCAGTG_nn'
Cuts at: 0 806 839 1497
Size: 806 33 658
Fragments arranged by size:
806 658 33
ClaI AT'CG_AT
Cuts at: 0 1244 1497
Size: 1244 253
DraIII CAC_nnn'GTG
Cuts at: 0 1170 1497
Size: 1170 327
EaeI y'GGCC_r
Cuts at: 0 194 699 836 1031 1108 1419 1434 1497
Size: 194 505 137 195 77 311 15 63
Fragments arranged by size:
505 311 195 194 137 77 63 15
EarI CTCTTCn'nnn_
Cuts at: 0 1076 1497
Size: 1076 421
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 244 1497
Size: 244 1253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 785 971 1078 1472 1497
Size: 55 730 186 107 394 25
Fragments arranged by size:
730 394 186 107 55 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 73 1497
Size: 73 1424
EcoO109I rG'GnC_Cy
Cuts at: 0 66 354 525 597 1410 1497
Size: 66 288 171 72 813 87
Fragments arranged by size:
813 288 171 87 72 66
FspI TGC'GCA
Cuts at: 0 1219 1497
Size: 1219 278
HaeII r_GCGC'y
Cuts at: 0 193 1160 1497
Size: 193 967 337
Fragments arranged by size:
967 337 193
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 159 191 655 687 807 839 1497
Size: 159 32 464 32 120 32 658
Fragments arranged by size:
658 464 159 120 32 32 32
KpnI G_GTAC'C
Cuts at: 0 860 1497
Size: 860 637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1066 1497
Size: 1066 431
MscI TGG'CCA
Cuts at: 0 196 701 1033 1497
Size: 196 505 332 464
Fragments arranged by size:
505 464 332 196
MslI CAynn'nnrTG
Cuts at: 0 295 421 505 964 1171 1497
Size: 295 126 84 459 207 326
Fragments arranged by size:
459 326 295 207 126 84
MspA1I CmG'CkG
Cuts at: 0 180 186 372 784 796 847 1424 1497
Size: 180 6 186 412 12 51 577 73
Fragments arranged by size:
577 412 186 180 73 51 12 6
NcoI C'CATG_G
Cuts at: 0 92 1497
Size: 92 1405
NspI r_CATG'y
Cuts at: 0 300 1497
Size: 300 1197
PasI CC'CwG_GG
Cuts at: 0 861 909 1497
Size: 861 48 588
Fragments arranged by size:
861 588 48
PciI A'CATG_T
Cuts at: 0 296 1497
Size: 296 1201
PflMI CCAn_nnn'nTGG
Cuts at: 0 909 1497
Size: 909 588
PfoI T'CCnGG_A
Cuts at: 0 1055 1497
Size: 1055 442
PpuMI rG'GwC_Cy
Cuts at: 0 354 525 597 1497
Size: 354 171 72 900
Fragments arranged by size:
900 354 171 72
PshAI GACnn'nnGTC
Cuts at: 0 879 1497
Size: 879 618
PstI C_TGCA'G
Cuts at: 0 167 804 1497
Size: 167 637 693
Fragments arranged by size:
693 637 167
PvuII CAG'CTG
Cuts at: 0 372 796 1497
Size: 372 424 701
Fragments arranged by size:
701 424 372
SanDI GG'GwC_CC
Cuts at: 0 597 1497
Size: 597 900
ScaI AGT'ACT
Cuts at: 0 706 1497
Size: 706 791
SfcI C'TryA_G
Cuts at: 0 163 800 1202 1497
Size: 163 637 402 295
Fragments arranged by size:
637 402 295 163
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1408 1497
Size: 1408 89
SmaI CCC'GGG
Cuts at: 0 595 1497
Size: 595 902
StuI AGG'CCT
Cuts at: 0 262 562 770 1497
Size: 262 300 208 727
Fragments arranged by size:
727 300 262 208
StyI C'CwwG_G
Cuts at: 0 92 377 1235 1497
Size: 92 285 858 262
Fragments arranged by size:
858 285 262 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 685 1497
Size: 685 812
TatI w'GTAC_w
Cuts at: 0 704 1497
Size: 704 793
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 833 865 1497
Size: 833 32 632
Fragments arranged by size:
833 632 32
Tth111I GACn'n_nGTC
Cuts at: 0 310 1497
Size: 310 1187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 165 1005 1414 1497
Size: 165 840 409 83
Fragments arranged by size:
840 409 165 83
XmaI C'CCGG_G
Cuts at: 0 593 1497
Size: 593 904
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI AlwNI ApaLI
AvaI BanI BanII BbsI BbvCI BglI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BstAPI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI DraIII
EaeI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I KpnI MmeI MscI MslI MspA1I NcoI NspI
PasI PciI PflMI PfoI PpuMI PshAI PstI PvuII
SanDI ScaI SfcI SfiI SmaI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AlfI AloI
ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BciVI BclI BfrBI BmgBI BmtI BplI
BpuEI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstZ17I
Bsu36I CspCI DraI DrdI EagI EcoICRI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KasI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI SbfI
SexAI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI XmnI ZraI