Restriction map for
pF1KB0481
(Linear) (Six-base) MAPSORT of: KIBB0481.seq Check: 1108 from: 1 to: 1563
KIBB0481.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AatII G_ACGT'C
Cuts at: 0 994 1563
Size: 994 569
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 1057 1431 1563
Size: 332 725 374 132
Fragments arranged by size:
725 374 332 132
AhdI GACnn_n'nnGTC
Cuts at: 0 990 1563
Size: 990 573
AlwNI CAG_nnn'CTG
Cuts at: 0 1386 1563
Size: 1386 177
ApaI G_GGCC'C
Cuts at: 0 346 1563
Size: 346 1217
ApoI r'AATT_y
Cuts at: 0 240 1299 1563
Size: 240 1059 264
Fragments arranged by size:
1059 264 240
AvaI C'yCGr_G
Cuts at: 0 524 582 682 1563
Size: 524 58 100 881
Fragments arranged by size:
881 524 100 58
BanI G'GyrC_C
Cuts at: 0 93 1079 1480 1563
Size: 93 986 401 83
Fragments arranged by size:
986 401 93 83
BanII G_rGCy'C
Cuts at: 0 346 571 1277 1563
Size: 346 225 706 286
Fragments arranged by size:
706 346 286 225
BbsI GAAGACnn'nnnn_
Cuts at: 0 7 1563
Size: 7 1556
BbvCI CC'TCA_GC
Cuts at: 0 1468 1563
Size: 1468 95
BlpI GC'TnA_GC
Cuts at: 0 1321 1403 1563
Size: 1321 82 160
Fragments arranged by size:
1321 160 82
Bme1580I G_kGCm'C
Cuts at: 0 66 98 211 346 1483 1563
Size: 66 32 113 135 1137 80
Fragments arranged by size:
1137 135 113 80 66 32
BmgBI CAC'GTC
Cuts at: 0 813 1563
Size: 813 750
BmrI ACTGGGnnnn_n'
Cuts at: 0 864 1563
Size: 864 699
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 171 1563
Size: 171 1392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 464 685 1459 1563
Size: 216 248 221 774 104
Fragments arranged by size:
774 248 221 216 104
Bpu10I CC'TnA_GC
Cuts at: 0 1468 1563
Size: 1468 95
BsaI GGTCTCn'nnnn_
Cuts at: 0 396 1563
Size: 396 1167
BsaBI GATnn'nnATC
Cuts at: 0 1417 1563
Size: 1417 146
BsaHI Gr'CG_yC
Cuts at: 0 84 991 1563
Size: 84 907 572
Fragments arranged by size:
907 572 84
BsaWI w'CCGG_w
Cuts at: 0 571 1349 1563
Size: 571 778 214
Fragments arranged by size:
778 571 214
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1153 1183 1563
Size: 1153 30 380
Fragments arranged by size:
1153 380 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1117 1563
Size: 1117 446
BseYI C'CCAG_C
Cuts at: 0 78 689 1367 1556 1563
Size: 78 611 678 189 7
Fragments arranged by size:
678 611 189 78 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 1063 1563
Size: 529 534 500
Fragments arranged by size:
534 529 500
BsmBI CGTCTCn'nnnn_
Cuts at: 0 91 196 1563
Size: 91 105 1367
Fragments arranged by size:
1367 105 91
Bsp1286I G_dGCh'C
Cuts at: 0 66 98 211 346 571 1277 1483 1563
Size: 66 32 113 135 225 706 206 80
Fragments arranged by size:
706 225 206 135 113 80 66 32
BspEI T'CCGG_A
Cuts at: 0 571 1349 1563
Size: 571 778 214
Fragments arranged by size:
778 571 214
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1050 1563
Size: 1050 513
BstXI CCAn_nnnn'nTGG
Cuts at: 0 253 1239 1316 1563
Size: 253 986 77 247
Fragments arranged by size:
986 253 247 77
BstYI r'GATC_y
Cuts at: 0 149 1563
Size: 149 1414
BtgI C'CryG_G
Cuts at: 0 894 1563
Size: 894 669
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1487 1563
Size: 1487 76
BtsI GCAGTG_nn'
Cuts at: 0 524 1012 1387 1563
Size: 524 488 375 176
Fragments arranged by size:
524 488 375 176
EaeI y'GGCC_r
Cuts at: 0 5 1100 1250 1563
Size: 5 1095 150 313
Fragments arranged by size:
1095 313 150 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1410 1563
Size: 1410 153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 332 464 685 1057 1431 1459 1563
Size: 216 116 132 221 372 374 28 104
Fragments arranged by size:
374 372 221 216 132 116 104 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 463 1563
Size: 463 1100
EcoO109I rG'GnC_Cy
Cuts at: 0 342 343 1563
Size: 342 1 1220
Fragments arranged by size:
1220 342 1
EcoRV GAT'ATC
Cuts at: 0 1189 1563
Size: 1189 374
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 241 951 1563
Size: 241 710 612
Fragments arranged by size:
710 612 241
HaeII r_GCGC'y
Cuts at: 0 654 1563
Size: 654 909
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 101 133 141 173 350 382 505 537
Size: 101 32 8 32 177 32 123 32
Cuts at: 537 976 1008 1382 1414 1442 1474 1563
Size: 439 32 374 32 28 32 89
Fragments arranged by size:
439 374 177 123 101 89 32 32
32 32 32 32 32 28 8
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 1485 1563
Size: 491 994 78
Fragments arranged by size:
994 491 78
MscI TGG'CCA
Cuts at: 0 7 1102 1252 1563
Size: 7 1095 150 311
Fragments arranged by size:
1095 311 150 7
MslI CAynn'nnrTG
Cuts at: 0 964 1016 1171 1432 1501 1563
Size: 964 52 155 261 69 62
Fragments arranged by size:
964 261 155 69 62 52
MspA1I CmG'CkG
Cuts at: 0 1116 1563
Size: 1116 447
PasI CC'CwG_GG
Cuts at: 0 68 374 1089 1563
Size: 68 306 715 474
Fragments arranged by size:
715 474 306 68
PflMI CCAn_nnn'nTGG
Cuts at: 0 785 1563
Size: 785 778
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 347 379 1563
Size: 347 32 1184
Fragments arranged by size:
1184 347 32
PspOMI G'GGCC_C
Cuts at: 0 342 1563
Size: 342 1221
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1327 1359 1563
Size: 1327 32 204
Fragments arranged by size:
1327 204 32
PstI C_TGCA'G
Cuts at: 0 1007 1184 1563
Size: 1007 177 379
Fragments arranged by size:
1007 379 177
PvuII CAG'CTG
Cuts at: 0 1116 1563
Size: 1116 447
SfcI C'TryA_G
Cuts at: 0 906 1003 1111 1180 1563
Size: 906 97 108 69 383
Fragments arranged by size:
906 383 108 97 69
StyI C'CwwG_G
Cuts at: 0 8 1563
Size: 8 1555
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 856 1563
Size: 856 707
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 14 46 114 146 203 235 1153 1185
Size: 14 32 68 32 57 32 918 32
Cuts at: 1185 1563
Size: 378
Fragments arranged by size:
918 378 68 57 32 32 32 32
14
Tth111I GACn'n_nGTC
Cuts at: 0 532 1563
Size: 532 1031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 75 892 1260 1563
Size: 75 817 368 303
Fragments arranged by size:
817 368 303 75
ZraI GAC'GTC
Cuts at: 0 992 1563
Size: 992 571
Enzymes that do cut:
AatII AcuI AhdI AlwNI ApaI ApoI AvaI BanI
BanII BbsI BbvCI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsmBI Bsp1286I BspEI BstAPI BstXI BstYI
BtgI BtgZI BtsI EaeI EciI Eco57MI EcoNI EcoO109I
EcoRV FalI HaeII Hin4I MmeI MscI MslI MspA1I
PasI PflMI PpiI PspOMI PsrI PstI PvuII SfcI
StyI TaqII TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglI
BglII BmtI BpuEI BsaAI BsiEI BsiHKAI BsiWI BsmI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EagI EarI EcoICRI EcoRI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PfoI PmeI PmlI PpuMI PshAI
PsiI PspXI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI XbaI XhoI XmaI XmnI