Restriction map for
pF1KB0485
(Linear) (Six-base) MAPSORT of: KIBB0485.seq Check: 5450 from: 1 to: 1584
KIBB0485.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 994 1289 1584
Size: 994 295 295
Fragments arranged by size:
994 295 295
AhdI GACnn_n'nnGTC
Cuts at: 0 671 1584
Size: 671 913
AleI CACnn'nnGTG
Cuts at: 0 844 1558 1584
Size: 844 714 26
Fragments arranged by size:
844 714 26
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 88 120 1584
Size: 88 32 1464
Fragments arranged by size:
1464 88 32
AlwNI CAG_nnn'CTG
Cuts at: 0 64 1494 1584
Size: 64 1430 90
Fragments arranged by size:
1430 90 64
AvaI C'yCGr_G
Cuts at: 0 72 115 467 1055 1584
Size: 72 43 352 588 529
Fragments arranged by size:
588 529 352 72 43
BanI G'GyrC_C
Cuts at: 0 67 333 1438 1533 1584
Size: 67 266 1105 95 51
Fragments arranged by size:
1105 266 95 67 51
BanII G_rGCy'C
Cuts at: 0 116 296 657 798 865 882 1096 1303
Size: 116 180 361 141 67 17 214 207
Cuts at: 1303 1377 1584
Size: 74 207
Fragments arranged by size:
361 214 207 207 180 141 116 74
67 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 818 1584
Size: 818 766
BbvCI CC'TCA_GC
Cuts at: 0 28 1584
Size: 28 1556
BglI GCCn_nnn'nGGC
Cuts at: 0 1091 1204 1584
Size: 1091 113 380
Fragments arranged by size:
1091 380 113
BglII A'GATC_T
Cuts at: 0 786 1584
Size: 786 798
BlpI GC'TnA_GC
Cuts at: 0 562 1584
Size: 562 1022
Bme1580I G_kGCm'C
Cuts at: 0 1416 1441 1476 1538 1565 1584
Size: 1416 25 35 62 27 19
Fragments arranged by size:
1416 62 35 27 25 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 427 660 1203 1426 1584
Size: 427 233 543 223 158
Fragments arranged by size:
543 427 233 223 158
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 762 1195 1584
Size: 762 433 389
Fragments arranged by size:
762 433 389
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 659 1584
Size: 300 359 925
Fragments arranged by size:
925 359 300
Bpu10I CC'TnA_GC
Cuts at: 0 28 1584
Size: 28 1556
BsaI GGTCTCn'nnnn_
Cuts at: 0 913 1168 1584
Size: 913 255 416
Fragments arranged by size:
913 416 255
BsaAI yAC'GTr
Cuts at: 0 1224 1485 1584
Size: 1224 261 99
Fragments arranged by size:
1224 261 99
BsaHI Gr'CG_yC
Cuts at: 0 242 1584
Size: 242 1342
BsaWI w'CCGG_w
Cuts at: 0 585 994 1523 1584
Size: 585 409 529 61
Fragments arranged by size:
585 529 409 61
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 448 1584
Size: 124 324 1136
Fragments arranged by size:
1136 324 124
BseYI C'CCAG_C
Cuts at: 0 1284 1294 1538 1584
Size: 1284 10 244 46
Fragments arranged by size:
1284 244 46 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1082 1465 1483 1507 1584
Size: 1082 383 18 24 77
Fragments arranged by size:
1082 383 77 24 18
BsiEI CG_ry'CG
Cuts at: 0 168 1464 1524 1584
Size: 168 1296 60 60
Fragments arranged by size:
1296 168 60 60
BsiHKAI G_wGCw'C
Cuts at: 0 1580 1584
Size: 1580 4
BsmI GAATG_Cn'
Cuts at: 0 886 1584
Size: 886 698
Bsp1286I G_dGCh'C
Cuts at: 0 116 296 657 798 865 882 1096 1303
Size: 116 180 361 141 67 17 214 207
Cuts at: 1303 1377 1416 1441 1476 1538 1565 1580 1584
Size: 74 39 25 35 62 27 15 4
Fragments arranged by size:
361 214 207 180 141 116 74 67
62 39 35 27 25 17 15 4
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 994 1289 1584
Size: 994 295 295
Fragments arranged by size:
994 295 295
BsrBI CCG'CTC
Cuts at: 0 1550 1584
Size: 1550 34
BsrDI GCAATG_nn'
Cuts at: 0 392 521 608 1584
Size: 392 129 87 976
Fragments arranged by size:
976 392 129 87
BsrFI r'CCGG_y
Cuts at: 0 151 1034 1466 1584
Size: 151 883 432 118
Fragments arranged by size:
883 432 151 118
BssSI C'ACGA_G
Cuts at: 0 774 1584
Size: 774 810
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1494 1584
Size: 1494 90
BstXI CCAn_nnnn'nTGG
Cuts at: 0 324 588 1584
Size: 324 264 996
Fragments arranged by size:
996 324 264
BstYI r'GATC_y
Cuts at: 0 786 1584
Size: 786 798
BtgI C'CryG_G
Cuts at: 0 89 233 734 1131 1565 1584
Size: 89 144 501 397 434 19
Fragments arranged by size:
501 434 397 144 89 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 492 1584
Size: 492 1092
BtsI GCAGTG_nn'
Cuts at: 0 600 839 1584
Size: 600 239 745
Fragments arranged by size:
745 600 239
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 504 539 1584
Size: 504 35 1045
Fragments arranged by size:
1045 504 35
DraIII CAC_nnn'GTG
Cuts at: 0 776 1584
Size: 776 808
DrdI GACnn_nn'nnGTC
Cuts at: 0 420 1182 1584
Size: 420 762 402
Fragments arranged by size:
762 420 402
EaeI y'GGCC_r
Cuts at: 0 2 165 539 686 1145 1205 1568 1584
Size: 2 163 374 147 459 60 363 16
Fragments arranged by size:
459 374 363 163 147 60 16 2
EagI C'GGCC_G
Cuts at: 0 165 1584
Size: 165 1419
EarI CTCTTCn'nnn_
Cuts at: 0 957 1114 1584
Size: 957 157 470
Fragments arranged by size:
957 470 157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 52 1030 1584
Size: 52 978 554
Fragments arranged by size:
978 554 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 659 1584
Size: 300 359 925
Fragments arranged by size:
925 359 300
EcoNI CCTnn'n_nnAGG
Cuts at: 0 31 1584
Size: 31 1553
EcoO109I rG'GnC_Cy
Cuts at: 0 120 471 1491 1584
Size: 120 351 1020 93
Fragments arranged by size:
1020 351 120 93
HaeII r_GCGC'y
Cuts at: 0 744 1584
Size: 744 840
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 271 303 657 689 934 966 1584
Size: 271 32 354 32 245 32 618
Fragments arranged by size:
618 354 271 245 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 1073 1584
Size: 35 1038 511
Fragments arranged by size:
1038 511 35
MscI TGG'CCA
Cuts at: 0 1147 1570 1584
Size: 1147 423 14
Fragments arranged by size:
1147 423 14
MslI CAynn'nnrTG
Cuts at: 0 586 844 1558 1584
Size: 586 258 714 26
Fragments arranged by size:
714 586 258 26
MspA1I CmG'CkG
Cuts at: 0 549 1479 1542 1584
Size: 549 930 63 42
Fragments arranged by size:
930 549 63 42
NaeI GCC'GGC
Cuts at: 0 1036 1468 1584
Size: 1036 432 116
Fragments arranged by size:
1036 432 116
NgoMIV G'CCGG_C
Cuts at: 0 1034 1466 1584
Size: 1034 432 118
Fragments arranged by size:
1034 432 118
PasI CC'CwG_GG
Cuts at: 0 831 1584
Size: 831 753
PflMI CCAn_nnn'nTGG
Cuts at: 0 64 776 1193 1284 1584
Size: 64 712 417 91 300
Fragments arranged by size:
712 417 300 91 64
PfoI T'CCnGG_A
Cuts at: 0 122 936 1584
Size: 122 814 648
Fragments arranged by size:
814 648 122
PmlI CAC'GTG
Cuts at: 0 1485 1584
Size: 1485 99
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 757 789 1584
Size: 757 32 795
Fragments arranged by size:
795 757 32
PpuMI rG'GwC_Cy
Cuts at: 0 120 1584
Size: 120 1464
PshAI GACnn'nnGTC
Cuts at: 0 997 1584
Size: 997 587
PstI C_TGCA'G
Cuts at: 0 143 1382 1584
Size: 143 1239 202
Fragments arranged by size:
1239 202 143
PvuII CAG'CTG
Cuts at: 0 1479 1584
Size: 1479 105
SapI GCTCTTCn'nnn_
Cuts at: 0 957 1584
Size: 957 627
SfcI C'TryA_G
Cuts at: 0 139 1378 1584
Size: 139 1239 206
Fragments arranged by size:
1239 206 139
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1204 1584
Size: 1204 380
SgrAI Cr'CCGG_yG
Cuts at: 0 1466 1584
Size: 1466 118
SmaI CCC'GGG
Cuts at: 0 74 469 1057 1584
Size: 74 395 588 527
Fragments arranged by size:
588 527 395 74
StuI AGG'CCT
Cuts at: 0 1258 1584
Size: 1258 326
StyI C'CwwG_G
Cuts at: 0 724 912 1584
Size: 724 188 672
Fragments arranged by size:
724 672 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 343 994 1006 1276 1584
Size: 343 651 12 270 308
Fragments arranged by size:
651 343 308 270 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1086 1584
Size: 1086 498
XmaI C'CCGG_G
Cuts at: 0 72 467 1055 1584
Size: 72 395 588 529
Fragments arranged by size:
588 529 395 72
Enzymes that do cut:
AarI AhdI AleI AloI AlwNI AvaI BanI BanII
BbsI BbvCI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI
CspCI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I HaeII Hin4I MmeI MscI MslI MspA1I
NaeI NgoMIV PasI PflMI PfoI PmlI PpiI PpuMI
PshAI PstI PvuII SapI SfcI SfiI SgrAI SmaI
StuI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlfI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI
BmgBI BmtI BpuEI BsaBI BsaXI BsiWI BsmBI BspEI
BspHI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I ClaI
DraI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PmeI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfoI
SgfI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI Tth111I XbaI XhoI XmnI ZraI