Restriction map for
pF1KB0491
(Linear) (Six-base) MAPSORT of: KIBB0491.seq Check: 1878 from: 1 to: 1779
KIBB0491.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 547 1779
Size: 547 1232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 891 1779
Size: 891 888
AfeI AGC'GCT
Cuts at: 0 1302 1779
Size: 1302 477
AhdI GACnn_n'nnGTC
Cuts at: 0 336 1779
Size: 336 1443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1485 1779
Size: 1485 294
AlwNI CAG_nnn'CTG
Cuts at: 0 219 390 1173 1779
Size: 219 171 783 606
Fragments arranged by size:
783 606 219 171
ApaI G_GGCC'C
Cuts at: 0 777 1779
Size: 777 1002
AvaI C'yCGr_G
Cuts at: 0 73 82 1228 1365 1570 1609 1719 1779
Size: 73 9 1146 137 205 39 110 60
Fragments arranged by size:
1146 205 137 110 73 60 39 9
BanI G'GyrC_C
Cuts at: 0 1279 1779
Size: 1279 500
BanII G_rGCy'C
Cuts at: 0 356 777 877 998 1469 1610 1645 1779
Size: 356 421 100 121 471 141 35 134
Fragments arranged by size:
471 421 356 141 134 121 100 35
BbeI G_GCGC'C
Cuts at: 0 1283 1779
Size: 1283 496
BbvCI CC'TCA_GC
Cuts at: 0 1684 1779
Size: 1684 95
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 271 305 1779
Size: 271 34 1474
Fragments arranged by size:
1474 271 34
BfrBI ATG'CAT
Cuts at: 0 1668 1779
Size: 1668 111
BglI GCCn_nnn'nGGC
Cuts at: 0 823 835 1066 1090 1779
Size: 823 12 231 24 689
Fragments arranged by size:
823 689 231 24 12
BlpI GC'TnA_GC
Cuts at: 0 274 1726 1779
Size: 274 1452 53
Fragments arranged by size:
1452 274 53
Bme1580I G_kGCm'C
Cuts at: 0 777 1779
Size: 777 1002
BmgBI CAC'GTC
Cuts at: 0 759 887 917 1779
Size: 759 128 30 862
Fragments arranged by size:
862 759 128 30
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 114 456 1659 1779
Size: 114 342 1203 120
Fragments arranged by size:
1203 342 120 114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 169 241 580 885 1099 1779
Size: 136 33 72 339 305 214 680
Fragments arranged by size:
680 339 305 214 136 72 33
Bpu10I CC'TnA_GC
Cuts at: 0 1261 1495 1604 1684 1779
Size: 1261 234 109 80 95
Fragments arranged by size:
1261 234 109 95 80
BsaI GGTCTCn'nnnn_
Cuts at: 0 79 716 1126 1779
Size: 79 637 410 653
Fragments arranged by size:
653 637 410 79
BsaHI Gr'CG_yC
Cuts at: 0 1254 1280 1779
Size: 1254 26 499
Fragments arranged by size:
1254 499 26
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1411 1429 1441 1459 1779
Size: 1411 18 12 18 320
Fragments arranged by size:
1411 320 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 271 274 724 817 820 823 826 937
Size: 271 3 450 93 3 3 3 111
Cuts at: 937 1779
Size: 842
Fragments arranged by size:
842 450 271 111 93 3 3 3
3
BseYI C'CCAG_C
Cuts at: 0 383 862 1163 1543 1691 1754 1779
Size: 383 479 301 380 148 63 25
Fragments arranged by size:
479 383 380 301 148 63 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 417 451 1597 1779
Size: 417 34 1146 182
Fragments arranged by size:
1146 417 182 34
BsiEI CG_ry'CG
Cuts at: 0 800 1005 1061 1130 1311 1386 1512 1779
Size: 800 205 56 69 181 75 126 267
Fragments arranged by size:
800 267 205 181 126 75 69 56
BsiHKAI G_wGCw'C
Cuts at: 0 356 935 998 1645 1779
Size: 356 579 63 647 134
Fragments arranged by size:
647 579 356 134 63
Bsp1286I G_dGCh'C
Cuts at: 0 356 777 877 935 998 1469 1610 1645
Size: 356 421 100 58 63 471 141 35
Cuts at: 1645 1779
Size: 134
Fragments arranged by size:
471 421 356 141 134 100 63 58
35
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 547 1779
Size: 547 1232
BsrBI CCG'CTC
Cuts at: 0 504 714 1398 1514 1779
Size: 504 210 684 116 265
Fragments arranged by size:
684 504 265 210 116
BsrDI GCAATG_nn'
Cuts at: 0 545 1779
Size: 545 1234
BsrFI r'CCGG_y
Cuts at: 0 725 1306 1779
Size: 725 581 473
Fragments arranged by size:
725 581 473
BssHII G'CGCG_C
Cuts at: 0 14 132 134 1779
Size: 14 118 2 1645
Fragments arranged by size:
1645 118 14 2
BssSI C'ACGA_G
Cuts at: 0 280 1779
Size: 280 1499
BstEII G'GTnAC_C
Cuts at: 0 321 1779
Size: 321 1458
BstXI CCAn_nnnn'nTGG
Cuts at: 0 558 1190 1779
Size: 558 632 589
Fragments arranged by size:
632 589 558
BtgI C'CryG_G
Cuts at: 0 652 1055 1061 1646 1705 1779
Size: 652 403 6 585 59 74
Fragments arranged by size:
652 585 403 74 59 6
BtsI GCAGTG_nn'
Cuts at: 0 196 400 690 1525 1779
Size: 196 204 290 835 254
Fragments arranged by size:
835 290 254 204 196
DraIII CAC_nnn'GTG
Cuts at: 0 426 1779
Size: 426 1353
EaeI y'GGCC_r
Cuts at: 0 797 1058 1127 1308 1383 1509 1779
Size: 797 261 69 181 75 126 270
Fragments arranged by size:
797 270 261 181 126 75 69
EagI C'GGCC_G
Cuts at: 0 797 1058 1127 1308 1383 1509 1779
Size: 797 261 69 181 75 126 270
Fragments arranged by size:
797 270 261 181 126 75 69
EarI CTCTTCn'nnn_
Cuts at: 0 1003 1779
Size: 1003 776
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 597 1122 1779
Size: 597 525 657
Fragments arranged by size:
657 597 525
EcoICRI GAG'CTC
Cuts at: 0 354 996 1643 1779
Size: 354 642 647 136
Fragments arranged by size:
647 642 354 136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 169 241 580 885 891 1099 1779
Size: 136 33 72 339 305 6 208 680
Fragments arranged by size:
680 339 305 208 136 72 33 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 411 1447 1747 1779
Size: 411 1036 300 32
Fragments arranged by size:
1036 411 300 32
EcoO109I rG'GnC_Cy
Cuts at: 0 1159 1170 1779
Size: 1159 11 609
Fragments arranged by size:
1159 609 11
FspI TGC'GCA
Cuts at: 0 43 172 1779
Size: 43 129 1607
Fragments arranged by size:
1607 129 43
HaeII r_GCGC'y
Cuts at: 0 914 1283 1304 1779
Size: 914 369 21 475
Fragments arranged by size:
914 475 369 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 322 354 1411 1429 1443 1461 1488 1520
Size: 322 32 1057 18 14 18 27 32
Cuts at: 1520 1779
Size: 259
Fragments arranged by size:
1057 322 259 32 32 27 18 18
14
KasI G'GCGC_C
Cuts at: 0 1279 1779
Size: 1279 500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 899 1779
Size: 899 880
MspA1I CmG'CkG
Cuts at: 0 387 654 1063 1077 1581 1707 1779
Size: 387 267 409 14 504 126 72
Fragments arranged by size:
504 409 387 267 126 72 14
NaeI GCC'GGC
Cuts at: 0 1308 1779
Size: 1308 471
NarI GG'CG_CC
Cuts at: 0 1280 1779
Size: 1280 499
NcoI C'CATG_G
Cuts at: 0 1646 1779
Size: 1646 133
NgoMIV G'CCGG_C
Cuts at: 0 1306 1779
Size: 1306 473
NsiI A_TGCA'T
Cuts at: 0 1670 1779
Size: 1670 109
PasI CC'CwG_GG
Cuts at: 0 1449 1779
Size: 1449 330
PflMI CCAn_nnn'nTGG
Cuts at: 0 114 219 1779
Size: 114 105 1560
Fragments arranged by size:
1560 114 105
PfoI T'CCnGG_A
Cuts at: 0 449 1779
Size: 449 1330
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 1779
Size: 76 32 1671
Fragments arranged by size:
1671 76 32
PpuMI rG'GwC_Cy
Cuts at: 0 1159 1779
Size: 1159 620
PspOMI G'GGCC_C
Cuts at: 0 773 1779
Size: 773 1006
PstI C_TGCA'G
Cuts at: 0 23 203 395 490 685 1327 1779
Size: 23 180 192 95 195 642 452
Fragments arranged by size:
642 452 195 192 180 95 23
PvuII CAG'CTG
Cuts at: 0 387 1581 1779
Size: 387 1194 198
Fragments arranged by size:
1194 387 198
SacI G_AGCT'C
Cuts at: 0 356 998 1645 1779
Size: 356 642 647 134
Fragments arranged by size:
647 642 356 134
SacII CC_GC'GG
Cuts at: 0 655 1064 1708 1779
Size: 655 409 644 71
Fragments arranged by size:
655 644 409 71
SanDI GG'GwC_CC
Cuts at: 0 1159 1779
Size: 1159 620
SapI GCTCTTCn'nnn_
Cuts at: 0 1003 1779
Size: 1003 776
SfcI C'TryA_G
Cuts at: 0 19 199 357 391 486 681 1323 1779
Size: 19 180 158 34 95 195 642 456
Fragments arranged by size:
642 456 195 180 158 95 34 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1090 1779
Size: 1090 689
SfoI GGC'GCC
Cuts at: 0 1281 1779
Size: 1281 498
SmaI CCC'GGG
Cuts at: 0 1230 1367 1779
Size: 1230 137 412
Fragments arranged by size:
1230 412 137
SmlI C'TyrA_G
Cuts at: 0 73 82 1779
Size: 73 9 1697
Fragments arranged by size:
1697 73 9
SrfI GCCC'GGGC
Cuts at: 0 1367 1779
Size: 1367 412
StuI AGG'CCT
Cuts at: 0 856 1654 1699 1779
Size: 856 798 45 80
Fragments arranged by size:
856 798 80 45
StyI C'CwwG_G
Cuts at: 0 317 551 1049 1631 1646 1748 1779
Size: 317 234 498 582 15 102 31
Fragments arranged by size:
582 498 317 234 102 31 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 103 769 1557 1779
Size: 103 666 788 222
Fragments arranged by size:
788 666 222 103
Tth111I GACn'n_nGTC
Cuts at: 0 529 957 1113 1779
Size: 529 428 156 666
Fragments arranged by size:
666 529 428 156
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1062 1629 1779
Size: 1062 567 150
Fragments arranged by size:
1062 567 150
XhoI C'TCGA_G
Cuts at: 0 73 82 1779
Size: 73 9 1697
Fragments arranged by size:
1697 73 9
XmaI C'CCGG_G
Cuts at: 0 1228 1365 1779
Size: 1228 137 414
Fragments arranged by size:
1228 414 137
Enzymes that do cut:
AarI AcuI AfeI AhdI AlfI AlwNI ApaI AvaI
BanI BanII BbeI BbvCI BcgI BfrBI BglI BlpI
Bme1580I BmgBI BplI BpmI Bpu10I BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstEII BstXI BtgI BtsI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I KasI MmeI MspA1I NaeI
NarI NcoI NgoMIV NsiI PasI PflMI PfoI PpiI
PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI
SfcI SfiI SfoI SmaI SmlI SrfI StuI StyI
TaqII Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BciVI BclI BglII BmrI BmtI BpuEI
BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI
BsrGI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EcoRI EcoRV FalI FseI FspAI
HincII HindIII HpaI KpnI MfeI MluI MscI MslI
NdeI NheI NotI NruI NspI PacI PciI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI
SspI SwaI TatI TstI XbaI XmnI ZraI