Restriction map for
pF1KB0494
(Linear) (Six-base) MAPSORT of: KIBB0494.seq Check: 4783 from: 1 to: 3117
KIBB0494.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2057 3117
Size: 2057 1060
AatII G_ACGT'C
Cuts at: 0 401 986 3117
Size: 401 585 2131
Fragments arranged by size:
2131 585 401
AccI GT'mk_AC
Cuts at: 0 1367 3117
Size: 1367 1750
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 588 1912 2548 2692 3117
Size: 588 1324 636 144 425
Fragments arranged by size:
1324 636 588 425 144
AflIII A'CryG_T
Cuts at: 0 737 2885 3117
Size: 737 2148 232
Fragments arranged by size:
2148 737 232
AleI CACnn'nnGTG
Cuts at: 0 1864 3117
Size: 1864 1253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2314 3117
Size: 2314 803
AlwNI CAG_nnn'CTG
Cuts at: 0 921 973 1222 1311 1336 1473 3117
Size: 921 52 249 89 25 137 1644
Fragments arranged by size:
1644 921 249 137 89 52 25
ApaI G_GGCC'C
Cuts at: 0 226 2079 3117
Size: 226 1853 1038
Fragments arranged by size:
1853 1038 226
ApaLI G'TGCA_C
Cuts at: 0 2044 3117
Size: 2044 1073
ApoI r'AATT_y
Cuts at: 0 896 3117
Size: 896 2221
AvaI C'yCGr_G
Cuts at: 0 705 2689 2700 2927 3117
Size: 705 1984 11 227 190
Fragments arranged by size:
1984 705 227 190 11
BamHI G'GATC_C
Cuts at: 0 2712 3117
Size: 2712 405
BanI G'GyrC_C
Cuts at: 0 205 859 1114 1360 1681 2059 2739 2907
Size: 205 654 255 246 321 378 680 168
Cuts at: 2907 3117
Size: 210
Fragments arranged by size:
680 654 378 321 255 246 210 205
168
BanII G_rGCy'C
Cuts at: 0 226 648 1443 1669 1783 1954 2079 2450
Size: 226 422 795 226 114 171 125 371
Cuts at: 2450 2690 3117
Size: 240 427
Fragments arranged by size:
795 427 422 371 240 226 226 171
125 114
BbeI G_GCGC'C
Cuts at: 0 209 2063 2743 3117
Size: 209 1854 680 374
Fragments arranged by size:
1854 680 374 209
BbsI GAAGACnn'nnnn_
Cuts at: 0 469 2473 3117
Size: 469 2004 644
Fragments arranged by size:
2004 644 469
BbvCI CC'TCA_GC
Cuts at: 0 1288 2488 3117
Size: 1288 1200 629
Fragments arranged by size:
1288 1200 629
BciVI GTATCCnnnnn_n'
Cuts at: 0 1378 3117
Size: 1378 1739
BglI GCCn_nnn'nGGC
Cuts at: 0 137 2703 3117
Size: 137 2566 414
Fragments arranged by size:
2566 414 137
BglII A'GATC_T
Cuts at: 0 2672 3117
Size: 2672 445
BlpI GC'TnA_GC
Cuts at: 0 491 1442 1777 1792 2917 3117
Size: 491 951 335 15 1125 200
Fragments arranged by size:
1125 951 491 335 200 15
Bme1580I G_kGCm'C
Cuts at: 0 226 262 289 864 1119 1580 1922 2048
Size: 226 36 27 575 255 461 342 126
Cuts at: 2048 2079 2912 3117
Size: 31 833 205
Fragments arranged by size:
833 575 461 342 255 226 205 126
36 31 27
BmgBI CAC'GTC
Cuts at: 0 1297 2310 3117
Size: 1297 1013 807
Fragments arranged by size:
1297 1013 807
BmrI ACTGGGnnnn_n'
Cuts at: 0 100 379 804 1235 1583 2616 3117
Size: 100 279 425 431 348 1033 501
Fragments arranged by size:
1033 501 431 425 348 279 100
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2715 3117
Size: 2715 402
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 65 285 411 671 750 2005 3117
Size: 61 4 220 126 260 79 1255 1112
Fragments arranged by size:
1255 1112 260 220 126 79 61 4
Bpu10I CC'TnA_GC
Cuts at: 0 544 1288 2080 2488 2939 3117
Size: 544 744 792 408 451 178
Fragments arranged by size:
792 744 544 451 408 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2581 3117
Size: 2581 536
BsaI GGTCTCn'nnnn_
Cuts at: 0 310 676 2128 3117
Size: 310 366 1452 989
Fragments arranged by size:
1452 989 366 310
BsaAI yAC'GTr
Cuts at: 0 558 738 3117
Size: 558 180 2379
Fragments arranged by size:
2379 558 180
BsaBI GATnn'nnATC
Cuts at: 0 2191 3117
Size: 2191 926
BsaHI Gr'CG_yC
Cuts at: 0 206 376 398 466 949 983 2060 2740
Size: 206 170 22 68 483 34 1077 680
Cuts at: 2740 3117
Size: 377
Fragments arranged by size:
1077 680 483 377 206 170 68 34
22
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 620 650 727 757 2227 2257 2875 2905
Size: 620 30 77 30 1470 30 618 30
Cuts at: 2905 2942 2972 3117
Size: 37 30 145
Fragments arranged by size:
1470 620 618 145 77 37 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 466 2986 3117
Size: 250 216 2520 131
Fragments arranged by size:
2520 250 216 131
BseYI C'CCAG_C
Cuts at: 0 226 575 1110 1120 1662 1922 2157 2230
Size: 226 349 535 10 542 260 235 73
Cuts at: 2230 2417 2513 2623 2827 3012 3117
Size: 187 96 110 204 185 105
Fragments arranged by size:
542 535 349 260 235 226 204 187
185 110 105 96 73 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 1888 2776 3117
Size: 130 1758 888 341
Fragments arranged by size:
1758 888 341 130
BsiEI CG_ry'CG
Cuts at: 0 383 507 1194 3117
Size: 383 124 687 1923
Fragments arranged by size:
1923 687 383 124
BsiHKAI G_wGCw'C
Cuts at: 0 1443 1793 2048 2438 2450 3117
Size: 1443 350 255 390 12 667
Fragments arranged by size:
1443 667 390 350 255 12
BsmI GAATG_Cn'
Cuts at: 0 963 3117
Size: 963 2154
BsmBI CGTCTCn'nnnn_
Cuts at: 0 232 3117
Size: 232 2885
Bsp1286I G_dGCh'C
Cuts at: 0 226 262 289 648 864 1119 1443 1580
Size: 226 36 27 359 216 255 324 137
Cuts at: 1580 1669 1783 1793 1922 1954 2048 2079 2438
Size: 89 114 10 129 32 94 31 359
Cuts at: 2438 2450 2690 2912 3117
Size: 12 240 222 205
Fragments arranged by size:
359 359 324 255 240 226 222 216
205 137 129 114 94 89 36 32
31 27 12 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2057 2534 2774 3117
Size: 2057 477 240 343
Fragments arranged by size:
2057 477 343 240
BsrBI CCG'CTC
Cuts at: 0 503 889 1959 2916 3117
Size: 503 386 1070 957 201
Fragments arranged by size:
1070 957 503 386 201
BsrDI GCAATG_nn'
Cuts at: 0 1211 2091 3117
Size: 1211 880 1026
Fragments arranged by size:
1211 1026 880
BsrFI r'CCGG_y
Cuts at: 0 1625 1646 2313 3080 3117
Size: 1625 21 667 767 37
Fragments arranged by size:
1625 767 667 37 21
BsrGI T'GTAC_A
Cuts at: 0 1433 3117
Size: 1433 1684
BssHII G'CGCG_C
Cuts at: 0 2779 3117
Size: 2779 338
BstAPI GCAn_nnn'nTGC
Cuts at: 0 930 1008 1222 3117
Size: 930 78 214 1895
Fragments arranged by size:
1895 930 214 78
BstXI CCAn_nnnn'nTGG
Cuts at: 0 201 1425 2458 2993 3117
Size: 201 1224 1033 535 124
Fragments arranged by size:
1224 1033 535 201 124
BstYI r'GATC_y
Cuts at: 0 831 1698 2672 2712 3117
Size: 831 867 974 40 405
Fragments arranged by size:
974 867 831 405 40
BtgI C'CryG_G
Cuts at: 0 180 211 611 812 1736 1817 2795 3117
Size: 180 31 400 201 924 81 978 322
Fragments arranged by size:
978 924 400 322 201 180 81 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1155 2212 3117
Size: 1155 1057 905
Fragments arranged by size:
1155 1057 905
BtsI GCAGTG_nn'
Cuts at: 0 19 1223 1934 2764 3117
Size: 19 1204 711 830 353
Fragments arranged by size:
1204 830 711 353 19
ClaI AT'CG_AT
Cuts at: 0 1373 3117
Size: 1373 1744
DrdI GACnn_nn'nnGTC
Cuts at: 0 2314 3117
Size: 2314 803
EaeI y'GGCC_r
Cuts at: 0 2 191 279 504 809 1058 1245 2039
Size: 2 189 88 225 305 249 187 794
Cuts at: 2039 3047 3082 3117
Size: 1008 35 35
Fragments arranged by size:
1008 794 305 249 225 189 187 88
35 35 2
EagI C'GGCC_G
Cuts at: 0 504 3117
Size: 504 2613
EarI CTCTTCn'nnn_
Cuts at: 0 1498 2219 3101 3117
Size: 1498 721 882 16
Fragments arranged by size:
1498 882 721 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1721 3117
Size: 1721 1396
EcoICRI GAG'CTC
Cuts at: 0 1441 2448 3117
Size: 1441 1007 669
Fragments arranged by size:
1441 1007 669
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 65 285 411 588 671 750 1912
Size: 61 4 220 126 177 83 79 1162
Cuts at: 1912 2005 2548 2692 3117
Size: 93 543 144 425
Fragments arranged by size:
1162 543 425 220 177 144 126 93
83 79 61 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 679 2053 3117
Size: 679 1374 1064
Fragments arranged by size:
1374 1064 679
EcoO109I rG'GnC_Cy
Cuts at: 0 64 130 223 1099 1237 1308 2021 2076
Size: 64 66 93 876 138 71 713 55
Cuts at: 2076 2422 2559 3117
Size: 346 137 558
Fragments arranged by size:
876 713 558 346 138 137 93 71
66 64 55
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3081 3117
Size: 3081 36
HaeII r_GCGC'y
Cuts at: 0 209 1022 2063 2743 3117
Size: 209 813 1041 680 374
Fragments arranged by size:
1041 813 680 374 209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 179 211 688 720 909 941 990 1022
Size: 179 32 477 32 189 32 49 32
Cuts at: 1022 1281 1313 2227 2259 3117
Size: 259 32 914 32 858
Fragments arranged by size:
914 858 477 259 189 179 49 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 987 2589 3117
Size: 987 1602 528
Fragments arranged by size:
1602 987 528
KasI G'GCGC_C
Cuts at: 0 205 2059 2739 3117
Size: 205 1854 680 378
Fragments arranged by size:
1854 680 378 205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1545 2546 3117
Size: 1545 1001 571
Fragments arranged by size:
1545 1001 571
MscI TGG'CCA
Cuts at: 0 4 193 3049 3117
Size: 4 189 2856 68
Fragments arranged by size:
2856 189 68 4
MslI CAynn'nnrTG
Cuts at: 0 199 556 1216 1555 1735 1864 2441 2456
Size: 199 357 660 339 180 129 577 15
Cuts at: 2456 3117
Size: 661
Fragments arranged by size:
661 660 577 357 339 199 180 129
15
MspA1I CmG'CkG
Cuts at: 0 213 389 1620 2253 2417 2790 2955 3117
Size: 213 176 1231 633 164 373 165 162
Fragments arranged by size:
1231 633 373 213 176 165 164 162
NaeI GCC'GGC
Cuts at: 0 1648 3117
Size: 1648 1469
NarI GG'CG_CC
Cuts at: 0 206 2060 2740 3117
Size: 206 1854 680 377
Fragments arranged by size:
1854 680 377 206
NcoI C'CATG_G
Cuts at: 0 180 1736 2795 3117
Size: 180 1556 1059 322
Fragments arranged by size:
1556 1059 322 180
NgoMIV G'CCGG_C
Cuts at: 0 1646 3117
Size: 1646 1471
NotI GC'GGCC_GC
Cuts at: 0 504 3117
Size: 504 2613
NruI TCG'CGA
Cuts at: 0 2197 3117
Size: 2197 920
NspI r_CATG'y
Cuts at: 0 2073 3117
Size: 2073 1044
PasI CC'CwG_GG
Cuts at: 0 219 882 1179 2055 2464 2553 3117
Size: 219 663 297 876 409 89 564
Fragments arranged by size:
876 663 564 409 297 219 89
PflMI CCAn_nnn'nTGG
Cuts at: 0 369 1110 1260 1311 1473 3117
Size: 369 741 150 51 162 1644
Fragments arranged by size:
1644 741 369 162 150 51
PfoI T'CCnGG_A
Cuts at: 0 1310 1766 1889 1982 2502 2537 3117
Size: 1310 456 123 93 520 35 580
Fragments arranged by size:
1310 580 520 456 123 93 35
PmlI CAC'GTG
Cuts at: 0 738 3117
Size: 738 2379
PpuMI rG'GwC_Cy
Cuts at: 0 64 1237 1308 2422 3117
Size: 64 1173 71 1114 695
Fragments arranged by size:
1173 1114 695 71 64
PspOMI G'GGCC_C
Cuts at: 0 222 2075 3117
Size: 222 1853 1042
Fragments arranged by size:
1853 1042 222
PspXI vC'TCGA_Gb
Cuts at: 0 2700 3117
Size: 2700 417
PstI C_TGCA'G
Cuts at: 0 1091 1619 1994 2258 2771 2867 3117
Size: 1091 528 375 264 513 96 250
Fragments arranged by size:
1091 528 513 375 264 250 96
PvuII CAG'CTG
Cuts at: 0 389 1620 2253 2417 2955 3117
Size: 389 1231 633 164 538 162
Fragments arranged by size:
1231 633 538 389 164 162
RsrII CG'GwC_CG
Cuts at: 0 2316 3117
Size: 2316 801
SacI G_AGCT'C
Cuts at: 0 1443 2450 3117
Size: 1443 1007 667
Fragments arranged by size:
1443 1007 667
SacII CC_GC'GG
Cuts at: 0 214 3117
Size: 214 2903
SanDI GG'GwC_CC
Cuts at: 0 1237 2422 3117
Size: 1237 1185 695
Fragments arranged by size:
1237 1185 695
SapI GCTCTTCn'nnn_
Cuts at: 0 1498 3117
Size: 1498 1619
SbfI CC_TGCA'GG
Cuts at: 0 2771 3117
Size: 2771 346
ScaI AGT'ACT
Cuts at: 0 520 781 3117
Size: 520 261 2336
Fragments arranged by size:
2336 520 261
SexAI A'CCwGG_T
Cuts at: 0 1421 3117
Size: 1421 1696
SfcI C'TryA_G
Cuts at: 0 842 1087 1615 1990 2254 2767 2863 3117
Size: 842 245 528 375 264 513 96 254
Fragments arranged by size:
842 528 513 375 264 254 245 96
SfoI GGC'GCC
Cuts at: 0 207 2061 2741 3117
Size: 207 1854 680 376
Fragments arranged by size:
1854 680 376 207
SmlI C'TyrA_G
Cuts at: 0 705 2596 2700 3117
Size: 705 1891 104 417
Fragments arranged by size:
1891 705 417 104
StuI AGG'CCT
Cuts at: 0 1459 2844 3117
Size: 1459 1385 273
Fragments arranged by size:
1459 1385 273
StyI C'CwwG_G
Cuts at: 0 180 363 1736 2795 2966 3117
Size: 180 183 1373 1059 171 151
Fragments arranged by size:
1373 1059 183 180 171 151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 36 369 1064 1102 1876 2985 2995 3004
Size: 36 333 695 38 774 1109 10 9
Cuts at: 3004 3117
Size: 113
Fragments arranged by size:
1109 774 695 333 113 38 36 10
9
TatI w'GTAC_w
Cuts at: 0 518 779 1433 2746 3117
Size: 518 261 654 1313 371
Fragments arranged by size:
1313 654 518 371 261
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 149 181 620 652 727 759 1871 1903
Size: 149 32 439 32 75 32 1112 32
Cuts at: 1903 2414 2446 2942 2974 3117
Size: 511 32 496 32 143
Fragments arranged by size:
1112 511 496 439 149 143 75 32
32 32 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1176 1561 1692 3042 3117
Size: 1176 385 131 1350 75
Fragments arranged by size:
1350 1176 385 131 75
XhoI C'TCGA_G
Cuts at: 0 705 2700 3117
Size: 705 1995 417
Fragments arranged by size:
1995 705 417
ZraI GAC'GTC
Cuts at: 0 399 984 3117
Size: 399 585 2133
Fragments arranged by size:
2133 585 399
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BstAPI BstXI BstYI BtgI BtgZI BtsI ClaI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NruI NspI
PasI PflMI PfoI PmlI PpuMI PspOMI PspXI PstI
PvuII RsrII SacI SacII SanDI SapI SbfI ScaI
SexAI SfcI SfoI SmlI StuI StyI TaqII TatI
TstI XcmI XhoI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AloI AscI
AseI AsiSI AvrII BaeI BcgI BclI BfrBI BmtI
BsaWI BsiWI BspEI BspHI BssSI BstBI BstEII BstZ17I
Bsu36I CspCI DraI DraIII EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI
NsiI PacI PciI PmeI PpiI PshAI PsiI PsrI
PvuI SalI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XmaI XmnI