Restriction map for
pF1KB0496
(Linear) (Six-base) MAPSORT of: KIBB0496.seq Check: 1340 from: 1 to: 3126
KIBB0496.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1048 3126
Size: 1048 2078
AccI GT'mk_AC
Cuts at: 0 2455 3126
Size: 2455 671
AclI AA'CG_TT
Cuts at: 0 138 2304 3126
Size: 138 2166 822
Fragments arranged by size:
2166 822 138
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 996 1436 2536 3126
Size: 281 715 440 1100 590
Fragments arranged by size:
1100 715 590 440 281
AflII C'TTAA_G
Cuts at: 0 267 534 1654 3126
Size: 267 267 1120 1472
Fragments arranged by size:
1472 1120 267 267
AflIII A'CryG_T
Cuts at: 0 1326 1722 1748 2565 3126
Size: 1326 396 26 817 561
Fragments arranged by size:
1326 817 561 396 26
AleI CACnn'nnGTG
Cuts at: 0 1367 3126
Size: 1367 1759
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1302 2828 3126
Size: 1302 1526 298
Fragments arranged by size:
1526 1302 298
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 394 426 3126
Size: 394 32 2700
Fragments arranged by size:
2700 394 32
AlwNI CAG_nnn'CTG
Cuts at: 0 118 2140 3126
Size: 118 2022 986
Fragments arranged by size:
2022 986 118
ApoI r'AATT_y
Cuts at: 0 613 758 1152 1403 1507 1818 2025 2124
Size: 613 145 394 251 104 311 207 99
Cuts at: 2124 2439 2748 3064 3126
Size: 315 309 316 62
Fragments arranged by size:
613 394 316 315 311 309 251 207
145 104 99 62
AseI AT'TA_AT
Cuts at: 0 653 1736 3126
Size: 653 1083 1390
Fragments arranged by size:
1390 1083 653
BamHI G'GATC_C
Cuts at: 0 2070 3126
Size: 2070 1056
BanI G'GyrC_C
Cuts at: 0 2993 3126
Size: 2993 133
BanII G_rGCy'C
Cuts at: 0 1633 1663 2657 3126
Size: 1633 30 994 469
Fragments arranged by size:
1633 994 469 30
BbeI G_GCGC'C
Cuts at: 0 2997 3126
Size: 2997 129
BbsI GAAGACnn'nnnn_
Cuts at: 0 269 3126
Size: 269 2857
BbvCI CC'TCA_GC
Cuts at: 0 489 3126
Size: 489 2637
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1030 1064 3126
Size: 1030 34 2062
Fragments arranged by size:
2062 1030 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 2182 3126
Size: 2182 944
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 63 3126
Size: 63 3063
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 2945 3126
Size: 95 2850 181
Fragments arranged by size:
2850 181 95
Bpu10I CC'TnA_GC
Cuts at: 0 398 489 3126
Size: 398 91 2637
Fragments arranged by size:
2637 398 91
BsaI GGTCTCn'nnnn_
Cuts at: 0 1070 1441 3126
Size: 1070 371 1685
Fragments arranged by size:
1685 1070 371
BsaAI yAC'GTr
Cuts at: 0 1723 3126
Size: 1723 1403
BsaHI Gr'CG_yC
Cuts at: 0 2994 3126
Size: 2994 132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2006 2036 3126
Size: 2006 30 1090
Fragments arranged by size:
2006 1090 30
BseYI C'CCAG_C
Cuts at: 0 2009 3126
Size: 2009 1117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 3126
Size: 464 2662
BsiEI CG_ry'CG
Cuts at: 0 2254 3126
Size: 2254 872
BsiHKAI G_wGCw'C
Cuts at: 0 2657 3126
Size: 2657 469
BsmI GAATG_Cn'
Cuts at: 0 10 2351 3126
Size: 10 2341 775
Fragments arranged by size:
2341 775 10
Bsp1286I G_dGCh'C
Cuts at: 0 1633 1663 2657 3126
Size: 1633 30 994 469
Fragments arranged by size:
1633 994 469 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1048 2169 3126
Size: 1048 1121 957
Fragments arranged by size:
1121 1048 957
BsrDI GCAATG_nn'
Cuts at: 0 521 943 1043 3126
Size: 521 422 100 2083
Fragments arranged by size:
2083 521 422 100
BsrFI r'CCGG_y
Cuts at: 0 2253 3126
Size: 2253 873
BsrGI T'GTAC_A
Cuts at: 0 328 3126
Size: 328 2798
BssSI C'ACGA_G
Cuts at: 0 380 3126
Size: 380 2746
BstAPI GCAn_nnn'nTGC
Cuts at: 0 349 2482 3126
Size: 349 2133 644
Fragments arranged by size:
2133 644 349
BstBI TT'CG_AA
Cuts at: 0 203 3126
Size: 203 2923
BstXI CCAn_nnnn'nTGG
Cuts at: 0 528 3126
Size: 528 2598
BstYI r'GATC_y
Cuts at: 0 2070 3126
Size: 2070 1056
BstZ17I GTA'TAC
Cuts at: 0 2456 3126
Size: 2456 670
Bsu36I CC'TnA_GG
Cuts at: 0 1316 3126
Size: 1316 1810
BtgI C'CryG_G
Cuts at: 0 1365 1442 3126
Size: 1365 77 1684
Fragments arranged by size:
1684 1365 77
BtsI GCAGTG_nn'
Cuts at: 0 53 2226 2649 3126
Size: 53 2173 423 477
Fragments arranged by size:
2173 477 423 53
DraI TTT'AAA
Cuts at: 0 2279 2886 3126
Size: 2279 607 240
Fragments arranged by size:
2279 607 240
DrdI GACnn_nn'nnGTC
Cuts at: 0 2795 3020 3126
Size: 2795 225 106
Fragments arranged by size:
2795 225 106
EaeI y'GGCC_r
Cuts at: 0 1067 2251 3126
Size: 1067 1184 875
Fragments arranged by size:
1184 1067 875
EagI C'GGCC_G
Cuts at: 0 2251 3126
Size: 2251 875
EarI CTCTTCn'nnn_
Cuts at: 0 242 350 1339 3126
Size: 242 108 989 1787
Fragments arranged by size:
1787 989 242 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1104 3126
Size: 19 1085 2022
Fragments arranged by size:
2022 1085 19
EcoICRI GAG'CTC
Cuts at: 0 2655 3126
Size: 2655 471
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 281 996 1436 2536 2945 3126
Size: 95 186 715 440 1100 409 181
Fragments arranged by size:
1100 715 440 409 186 181 95
EcoO109I rG'GnC_Cy
Cuts at: 0 106 3126
Size: 106 3020
EcoRI G'AATT_C
Cuts at: 0 1818 2748 3126
Size: 1818 930 378
Fragments arranged by size:
1818 930 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3025 3126
Size: 3025 101
HaeII r_GCGC'y
Cuts at: 0 2997 3126
Size: 2997 129
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1276 1308 1548 1580 2482 2514 3126
Size: 1276 32 240 32 902 32 612
Fragments arranged by size:
1276 902 612 240 32 32 32
HincII GTy'rAC
Cuts at: 0 453 3126
Size: 453 2673
HindIII A'AGCT_T
Cuts at: 0 2393 3126
Size: 2393 733
KasI G'GCGC_C
Cuts at: 0 2993 3126
Size: 2993 133
MfeI C'AATT_G
Cuts at: 0 1280 1637 3126
Size: 1280 357 1489
Fragments arranged by size:
1489 1280 357
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 1182 1733 3126
Size: 366 816 551 1393
Fragments arranged by size:
1393 816 551 366
MscI TGG'CCA
Cuts at: 0 1069 3126
Size: 1069 2057
MslI CAynn'nnrTG
Cuts at: 0 1367 1555 3126
Size: 1367 188 1571
Fragments arranged by size:
1571 1367 188
MspA1I CmG'CkG
Cuts at: 0 493 1666 3126
Size: 493 1173 1460
Fragments arranged by size:
1460 1173 493
NarI GG'CG_CC
Cuts at: 0 2994 3126
Size: 2994 132
NcoI C'CATG_G
Cuts at: 0 1365 3126
Size: 1365 1761
NspI r_CATG'y
Cuts at: 0 1330 1473 1752 3126
Size: 1330 143 279 1374
Fragments arranged by size:
1374 1330 279 143
PciI A'CATG_T
Cuts at: 0 1326 1748 3126
Size: 1326 422 1378
Fragments arranged by size:
1378 1326 422
PflMI CCAn_nnn'nTGG
Cuts at: 0 385 1639 3126
Size: 385 1254 1487
Fragments arranged by size:
1487 1254 385
PfoI T'CCnGG_A
Cuts at: 0 1794 3126
Size: 1794 1332
PshAI GACnn'nnGTC
Cuts at: 0 2959 3126
Size: 2959 167
PsiI TTA'TAA
Cuts at: 0 1880 3126
Size: 1880 1246
PstI C_TGCA'G
Cuts at: 0 123 354 2706 3126
Size: 123 231 2352 420
Fragments arranged by size:
2352 420 231 123
SacI G_AGCT'C
Cuts at: 0 2657 3126
Size: 2657 469
ScaI AGT'ACT
Cuts at: 0 2093 3126
Size: 2093 1033
SexAI A'CCwGG_T
Cuts at: 0 1965 3126
Size: 1965 1161
SfcI C'TryA_G
Cuts at: 0 119 350 1397 2103 2132 2702 3126
Size: 119 231 1047 706 29 570 424
Fragments arranged by size:
1047 706 570 424 231 119 29
SfoI GGC'GCC
Cuts at: 0 2995 3126
Size: 2995 131
SmlI C'TyrA_G
Cuts at: 0 267 534 1654 3126
Size: 267 267 1120 1472
Fragments arranged by size:
1472 1120 267 267
SspI AAT'ATT
Cuts at: 0 557 3126
Size: 557 2569
StuI AGG'CCT
Cuts at: 0 1432 3126
Size: 1432 1694
StyI C'CwwG_G
Cuts at: 0 1365 3126
Size: 1365 1761
TatI w'GTAC_w
Cuts at: 0 328 1329 1745 2091 3126
Size: 328 1001 416 346 1035
Fragments arranged by size:
1035 1001 416 346 328
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2006 2038 3126
Size: 2006 32 1088
Fragments arranged by size:
2006 1088 32
XmnI GAAnn'nnTTC
Cuts at: 0 614 1522 1550 1928 2093 2412 3008 3126
Size: 614 908 28 378 165 319 596 118
Fragments arranged by size:
908 614 596 378 319 165 118 28
Enzymes that do cut:
AarI AccI AclI AcuI AflII AflIII AleI AlfI
AloI AlwNI ApoI AseI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BmrI BplI BpmI Bpu10I BsaI
BsaAI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI HaeII Hin4I HincII HindIII KasI MfeI MmeI
MscI MslI MspA1I NarI NcoI NspI PciI PflMI
PfoI PshAI PsiI PstI SacI ScaI SexAI SfcI
SfoI SmlI SspI StuI StyI TatI TstI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AgeI AhdI ApaI ApaLI AscI
AsiSI AvaI AvrII BaeI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmtI BpuEI BsaBI BsaWI
BseRI BsiWI BsmBI BspEI BspHI BsrBI BssHII BstEII
BtgZI ClaI CspCI DraIII EcoNI EcoRV FseI FspI
FspAI HpaI KpnI MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PmeI PmlI PpiI
PpuMI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SwaI TaqII Tth111I XbaI
XcmI XhoI XmaI ZraI