Restriction map for
pF1KB0497
(Linear) (Six-base) MAPSORT of: KIBB0497.seq Check: 5545 from: 1 to: 13281
KIBB0497.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2659 5390 6591 8586 11029 11730 13281
Size: 2659 2731 1201 1995 2443 701 1551
Fragments arranged by size:
2731 2659 2443 1995 1551 1201 701
AccI GT'mk_AC
Cuts at: 0 416 2193 2312 4231 6674 7178 7406 8810
Size: 416 1777 119 1919 2443 504 228 1404
Cuts at: 8810 11009 11293 12661 13281
Size: 2199 284 1368 620
Fragments arranged by size:
2443 2199 1919 1777 1404 1368 620 504
416 284 228 119
AclI AA'CG_TT
Cuts at: 0 12902 13281
Size: 12902 379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 1045 3181 3262 3433 3643 4052 6032
Size: 391 654 2136 81 171 210 409 1980
Cuts at: 6032 7705 8110 8155 8679 9526 9827 9841 10015
Size: 1673 405 45 524 847 301 14 174
Cuts at: 10015 10261 10303 12869 13281
Size: 246 42 2566 412
Fragments arranged by size:
2566 2136 1980 1673 847 654 524 412
409 405 391 301 246 210 174 171
81 45 42 14
AfeI AGC'GCT
Cuts at: 0 7804 11587 13281
Size: 7804 3783 1694
Fragments arranged by size:
7804 3783 1694
AflII C'TTAA_G
Cuts at: 0 12106 13281
Size: 12106 1175
AflIII A'CryG_T
Cuts at: 0 2879 3536 7331 7472 7781 8640 11872 12141
Size: 2879 657 3795 141 309 859 3232 269
Cuts at: 12141 13281
Size: 1140
Fragments arranged by size:
3795 3232 2879 1140 859 657 309 269
141
AgeI A'CCGG_T
Cuts at: 0 12227 13281
Size: 12227 1054
AhdI GACnn_n'nnGTC
Cuts at: 0 1811 5505 7615 13212 13281
Size: 1811 3694 2110 5597 69
Fragments arranged by size:
5597 3694 2110 1811 69
AleI CACnn'nnGTG
Cuts at: 0 10753 12871 13281
Size: 10753 2118 410
Fragments arranged by size:
10753 2118 410
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 125 4411 6607 9613 10582 11371 12362 13225
Size: 125 4286 2196 3006 969 789 991 863
Cuts at: 13225 13281
Size: 56
Fragments arranged by size:
4286 3006 2196 991 969 863 789 125
56
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 820 852 6520 6552 13281
Size: 820 32 5668 32 6729
Fragments arranged by size:
6729 5668 820 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1419 3201 3666 4206 4436 4660 5616 5898
Size: 1419 1782 465 540 230 224 956 282
Cuts at: 5898 7296 8310 8702 8997 9237 9897 10203 10239
Size: 1398 1014 392 295 240 660 306 36
Cuts at: 10239 10539 10554 13281
Size: 300 15 2727
Fragments arranged by size:
2727 1782 1419 1398 1014 956 660 540
465 392 306 300 295 282 240 230
224 36 15
ApaI G_GGCC'C
Cuts at: 0 496 4730 7597 9303 12813 13281
Size: 496 4234 2867 1706 3510 468
Fragments arranged by size:
4234 3510 2867 1706 496 468
ApaLI G'TGCA_C
Cuts at: 0 1651 1741 13281
Size: 1651 90 11540
Fragments arranged by size:
11540 1651 90
ApoI r'AATT_y
Cuts at: 0 810 2326 2641 3430 3502 5147 8548 13281
Size: 810 1516 315 789 72 1645 3401 4733
Fragments arranged by size:
4733 3401 1645 1516 810 789 315 72
AvaI C'yCGr_G
Cuts at: 0 77 214 245 1139 2127 4761 5156 5316
Size: 77 137 31 894 988 2634 395 160
Cuts at: 5316 6595 6835 8261 8585 8779 10157 12446 13281
Size: 1279 240 1426 324 194 1378 2289 835
Fragments arranged by size:
2634 2289 1426 1378 1279 988 894 835
395 324 240 194 160 137 77 31
AvrII C'CTAG_G
Cuts at: 0 4371 6378 13281
Size: 4371 2007 6903
Fragments arranged by size:
6903 4371 2007
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 6831 6864 12094 12127 13281
Size: 6831 33 5230 33 1154
Fragments arranged by size:
6831 5230 1154 33 33
BamHI G'GATC_C
Cuts at: 0 6488 7931 8688 8705 11748 13281
Size: 6488 1443 757 17 3043 1533
Fragments arranged by size:
6488 3043 1533 1443 757 17
BanI G'GyrC_C
Cuts at: 0 945 1240 3134 3318 5787 6825 7167 7699
Size: 945 295 1894 184 2469 1038 342 532
Cuts at: 7699 8523 8598 11282 12164 13281
Size: 824 75 2684 882 1117
Fragments arranged by size:
2684 2469 1894 1117 1038 945 882 824
532 342 295 184 75
BanII G_rGCy'C
Cuts at: 0 149 215 496 1616 2265 3680 4730 5398
Size: 149 66 281 1120 649 1415 1050 668
Cuts at: 5398 6755 7039 7597 8099 8435 9303 9413 9644
Size: 1357 284 558 502 336 868 110 231
Cuts at: 9644 12813 13058 13281
Size: 3169 245 223
Fragments arranged by size:
3169 1415 1357 1120 1050 868 668 649
558 502 336 284 281 245 231 223
149 110 66
BbeI G_GCGC'C
Cuts at: 0 949 3138 12168 13281
Size: 949 2189 9030 1113
Fragments arranged by size:
9030 2189 1113 949
BbsI GAAGACnn'nnnn_
Cuts at: 0 1370 2418 2709 3369 3748 4385 7270 7944
Size: 1370 1048 291 660 379 637 2885 674
Cuts at: 7944 8117 8143 12875 13281
Size: 173 26 4732 406
Fragments arranged by size:
4732 2885 1370 1048 674 660 637 406
379 291 173 26
BbvCI CC'TCA_GC
Cuts at: 0 1428 6406 6743 8509 8674 9704 10494 13281
Size: 1428 4978 337 1766 165 1030 790 2787
Fragments arranged by size:
4978 2787 1766 1428 1030 790 337 165
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3277 3311 5565 5599 6136 6170 11254 11288
Size: 3277 34 2254 34 537 34 5084 34
Cuts at: 11288 13281
Size: 1993
Fragments arranged by size:
5084 3277 2254 1993 537 34 34 34
34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1342 1836 3526 6666 10297 12977 13281
Size: 1342 494 1690 3140 3631 2680 304
Fragments arranged by size:
3631 3140 2680 1690 1342 494 304
BclI T'GATC_A
Cuts at: 0 1091 1421 3981 4952 7433 7706 9467 13233
Size: 1091 330 2560 971 2481 273 1761 3766
Cuts at: 13233 13281
Size: 48
Fragments arranged by size:
3766 2560 2481 1761 1091 971 330 273
48
BfrBI ATG'CAT
Cuts at: 0 854 7830 13281
Size: 854 6976 5451
Fragments arranged by size:
6976 5451 854
BglI GCCn_nnn'nGGC
Cuts at: 0 79 5414 6646 8320 9298 10837 12808 13281
Size: 79 5335 1232 1674 978 1539 1971 473
Fragments arranged by size:
5335 1971 1674 1539 1232 978 473 79
BglII A'GATC_T
Cuts at: 0 545 2891 4058 6084 8852 9258 10019 13281
Size: 545 2346 1167 2026 2768 406 761 3262
Fragments arranged by size:
3262 2768 2346 2026 1167 761 545 406
BlpI GC'TnA_GC
Cuts at: 0 143 1985 5218 9674 11776 12061 13281
Size: 143 1842 3233 4456 2102 285 1220
Fragments arranged by size:
4456 3233 2102 1842 1220 285 143
Bme1580I G_kGCm'C
Cuts at: 0 496 653 786 1245 1655 1745 4730 5937
Size: 496 157 133 459 410 90 2985 1207
Cuts at: 5937 6830 7172 7597 8192 8528 9303 10176 11550
Size: 893 342 425 595 336 775 873 1374
Cuts at: 11550 12813 13255 13281
Size: 1263 442 26
Fragments arranged by size:
2985 1374 1263 1207 893 873 775 595
496 459 442 425 410 342 336 157
133 90 26
BmgBI CAC'GTC
Cuts at: 0 1452 8444 11875 13281
Size: 1452 6992 3431 1406
Fragments arranged by size:
6992 3431 1452 1406
BmrI ACTGGGnnnn_n'
Cuts at: 0 1620 1947 2157 3220 3726 3855 3954 4293
Size: 1620 327 210 1063 506 129 99 339
Cuts at: 4293 5247 7268 11211 11676 12165 13281
Size: 954 2021 3943 465 489 1116
Fragments arranged by size:
3943 2021 1620 1116 1063 954 506 489
465 339 327 210 129 99
BmtI G_CTAG'C
Cuts at: 0 12535 13281
Size: 12535 746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 844 1294 1630 3163 3223 3361 3673
Size: 664 180 450 336 1533 60 138 312
Cuts at: 3673 3688 4648 5564 6745 7287 7516 9103 9325
Size: 15 960 916 1181 542 229 1587 222
Cuts at: 9325 9763 9847 9919 10561 10609 10672 10789 10984
Size: 438 84 72 642 48 63 117 195
Cuts at: 10984 11503 12262 12455 13281
Size: 519 759 193 826
Fragments arranged by size:
1587 1533 1181 960 916 826 759 664
642 542 519 450 438 336 312 229
222 195 193 180 138 117 84 72
63 60 48 15
Bpu10I CC'TnA_GC
Cuts at: 0 164 1085 1428 3115 6406 6743 7466 8509
Size: 164 921 343 1687 3291 337 723 1043
Cuts at: 8509 8514 8674 9704 10494 10583 13281
Size: 5 160 1030 790 89 2698
Fragments arranged by size:
3291 2698 1687 1043 1030 921 790 723
343 337 164 160 89 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 2119 2851 3043 5020 8419 10627 12484
Size: 170 1949 732 192 1977 3399 2208 1857
Cuts at: 12484 13281
Size: 797
Fragments arranged by size:
3399 2208 1977 1949 1857 797 732 192
170
BsaI GGTCTCn'nnnn_
Cuts at: 0 730 3775 4567 5425 6130 6317 10483 10765
Size: 730 3045 792 858 705 187 4166 282
Cuts at: 10765 10774 12652 13281
Size: 9 1878 629
Fragments arranged by size:
4166 3045 1878 858 792 730 705 629
282 187 9
BsaAI yAC'GTr
Cuts at: 0 1620 1971 7410 11131 13281
Size: 1620 351 5439 3721 2150
Fragments arranged by size:
5439 3721 2150 1620 351
BsaBI GATnn'nnATC
Cuts at: 0 11368 11753 13281
Size: 11368 385 1528
Fragments arranged by size:
11368 1528 385
BsaHI Gr'CG_yC
Cuts at: 0 946 1665 1960 3135 8301 10124 12165 13281
Size: 946 719 295 1175 5166 1823 2041 1116
Fragments arranged by size:
5166 2041 1823 1175 1116 946 719 295
BsaWI w'CCGG_w
Cuts at: 0 1633 4481 4874 12227 13025 13281
Size: 1633 2848 393 7353 798 256
Fragments arranged by size:
7353 2848 1633 798 393 256
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 820 850 2896 2926 6520 6550 10114 10144
Size: 820 30 2046 30 3594 30 3564 30
Cuts at: 10144 11422 11452 12904 12934 13171 13201 13281
Size: 1278 30 1452 30 237 30 80
Fragments arranged by size:
3594 3564 2046 1452 1278 820 237 80
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 841 3025 3736 7006 9142 9802 9895
Size: 172 669 2184 711 3270 2136 660 93
Cuts at: 9895 9916 10606 11629 12100 13281
Size: 21 690 1023 471 1181
Fragments arranged by size:
3270 2184 2136 1181 1023 711 690 669
660 471 172 93 21
BseYI C'CCAG_C
Cuts at: 0 381 1199 1646 3130 4729 6906 7484 8056
Size: 381 818 447 1484 1599 2177 578 572
Cuts at: 8056 8422 8718 8976 9357 9530 9835 10547 10554
Size: 366 296 258 381 173 305 712 7
Cuts at: 10554 10832 11582 13019 13281
Size: 278 750 1437 262
Fragments arranged by size:
2177 1599 1484 1437 818 750 712 578
572 447 381 381 366 305 296 278
262 258 173 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 586 904 1288 2128 2230 2368 3022
Size: 493 93 318 384 840 102 138 654
Cuts at: 3022 3094 3133 4357 4684 5368 5911 6237 6482
Size: 72 39 1224 327 684 543 326 245
Cuts at: 6482 7381 8641 8665 8718 9169 10348 10729 13281
Size: 899 1260 24 53 451 1179 381 2552
Fragments arranged by size:
2552 1260 1224 1179 899 840 684 654
543 493 451 384 381 327 326 318
245 138 102 93 72 53 39 24
BsiEI CG_ry'CG
Cuts at: 0 9561 13281
Size: 9561 3720
BsiHKAI G_wGCw'C
Cuts at: 0 149 1655 1704 1745 3680 4489 7322 8207
Size: 149 1506 49 41 1935 809 2833 885
Cuts at: 8207 8435 8669 10862 11570 13281
Size: 228 234 2193 708 1711
Fragments arranged by size:
2833 2193 1935 1711 1506 885 809 708
234 228 149 49 41
BsiWI C'GTAC_G
Cuts at: 0 7540 13281
Size: 7540 5741
BsmI GAATG_Cn'
Cuts at: 0 114 1829 2006 6284 13281
Size: 114 1715 177 4278 6997
Fragments arranged by size:
6997 4278 1715 177 114
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1951 9570 13281
Size: 1951 7619 3711
Fragments arranged by size:
7619 3711 1951
Bsp1286I G_dGCh'C
Cuts at: 0 149 215 496 653 786 1245 1616 1655
Size: 149 66 281 157 133 459 371 39
Cuts at: 1655 1704 1745 2265 3680 4489 4730 5398 5937
Size: 49 41 520 1415 809 241 668 539
Cuts at: 5937 6755 6830 7039 7172 7322 7597 8099 8192
Size: 818 75 209 133 150 275 502 93
Cuts at: 8192 8207 8435 8528 8669 9303 9413 9644 10176
Size: 15 228 93 141 634 110 231 532
Cuts at: 10176 10862 11550 11570 12813 13058 13255 13281
Size: 686 688 20 1243 245 197 26
Fragments arranged by size:
1415 1243 818 809 688 686 668 634
539 532 520 502 459 371 281 275
245 241 231 228 209 197 157 150
149 141 133 133 110 93 93 75
66 49 41 39 26 20 15
BspEI T'CCGG_A
Cuts at: 0 1633 4481 4874 13281
Size: 1633 2848 393 8407
Fragments arranged by size:
8407 2848 1633 393
BspHI T'CATG_A
Cuts at: 0 1052 1682 2405 5042 11249 13265 13281
Size: 1052 630 723 2637 6207 2016 16
Fragments arranged by size:
6207 2637 2016 1052 723 630 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1696 2009 2390 2659 3923 4631 5390 6591
Size: 1696 313 381 269 1264 708 759 1201
Cuts at: 6591 8586 8663 10262 10280 11029 11730 11808 11931
Size: 1995 77 1599 18 749 701 78 123
Cuts at: 11931 13217 13281
Size: 1286 64
Fragments arranged by size:
1995 1696 1599 1286 1264 1201 759 749
708 701 381 313 269 123 78 77
64 18
BsrBI CCG'CTC
Cuts at: 0 1944 5016 10578 12714 13281
Size: 1944 3072 5562 2136 567
Fragments arranged by size:
5562 3072 2136 1944 567
BsrDI GCAATG_nn'
Cuts at: 0 1849 2969 3090 4109 5374 5804 6641 11355
Size: 1849 1120 121 1019 1265 430 837 4714
Cuts at: 11355 12116 13281
Size: 761 1165
Fragments arranged by size:
4714 1849 1265 1165 1120 1019 837 761
430 121
BsrFI r'CCGG_y
Cuts at: 0 3749 5321 5641 7961 10687 12121 12161 12227
Size: 3749 1572 320 2320 2726 1434 40 66
Cuts at: 12227 13281
Size: 1054
Fragments arranged by size:
3749 2726 2320 1572 1434 1054 320 66
40
BsrGI T'GTAC_A
Cuts at: 0 393 2361 2468 5867 7784 13281
Size: 393 1968 107 3399 1917 5497
Fragments arranged by size:
5497 3399 1968 1917 393 107
BssHII G'CGCG_C
Cuts at: 0 1642 13281
Size: 1642 11639
BssSI C'ACGA_G
Cuts at: 0 3229 3286 3710 9844 11273 11509 13281
Size: 3229 57 424 6134 1429 236 1772
Fragments arranged by size:
6134 3229 1772 1429 424 236 57
BstAPI GCAn_nnn'nTGC
Cuts at: 0 851 1311 1464 2463 7296 8202 8664 8997
Size: 851 460 153 999 4833 906 462 333
Cuts at: 8997 11364 13281
Size: 2367 1917
Fragments arranged by size:
4833 2367 1917 999 906 851 462 460
333 153
BstBI TT'CG_AA
Cuts at: 0 1785 3500 6770 11036 13281
Size: 1785 1715 3270 4266 2245
Fragments arranged by size:
4266 3270 2245 1785 1715
BstEII G'GTnAC_C
Cuts at: 0 2397 4854 8361 13281
Size: 2397 2457 3507 4920
Fragments arranged by size:
4920 3507 2457 2397
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3528 3579 4720 5665 6246 7239 8946 11082
Size: 3528 51 1141 945 581 993 1707 2136
Cuts at: 11082 11333 12780 13281
Size: 251 1447 501
Fragments arranged by size:
3528 2136 1707 1447 1141 993 945 581
501 251 51
BstYI r'GATC_y
Cuts at: 0 545 971 1539 2120 2807 2891 3272 3965
Size: 545 426 568 581 687 84 381 693
Cuts at: 3965 4058 4967 5247 5612 5699 5985 6084 6488
Size: 93 909 280 365 87 286 99 404
Cuts at: 6488 7931 8121 8558 8688 8705 8852 9258 10019
Size: 1443 190 437 130 17 147 406 761
Cuts at: 10019 10661 11748 12491 12575 13281
Size: 642 1087 743 84 706
Fragments arranged by size:
1443 1087 909 761 743 706 693 687
642 581 568 545 437 426 406 404
381 365 286 280 190 147 130 99
93 87 84 84 17
BstZ17I GTA'TAC
Cuts at: 0 2194 2313 11294 13281
Size: 2194 119 8981 1987
Fragments arranged by size:
8981 2194 1987 119
Bsu36I CC'TnA_GG
Cuts at: 0 4894 10190 12323 13281
Size: 4894 5296 2133 958
Fragments arranged by size:
5296 4894 2133 958
BtgI C'CryG_G
Cuts at: 0 169 3776 3785 3792 5369 5510 5780 6506
Size: 169 3607 9 7 1577 141 270 726
Cuts at: 6506 6932 7250 7589 7793 8681 9605 11241 13281
Size: 426 318 339 204 888 924 1636 2040
Fragments arranged by size:
3607 2040 1636 1577 924 888 726 426
339 318 270 204 169 141 9 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2908 4847 6018 6570 6604 7257 10605 11332
Size: 2908 1939 1171 552 34 653 3348 727
Cuts at: 11332 11610 13281
Size: 278 1671
Fragments arranged by size:
3348 2908 1939 1671 1171 727 653 552
278 34
BtsI GCAGTG_nn'
Cuts at: 0 281 2464 2527 3078 4955 9127 9271 12866
Size: 281 2183 63 551 1877 4172 144 3595
Cuts at: 12866 13253 13281
Size: 387 28
Fragments arranged by size:
4172 3595 2183 1877 551 387 281 144
63 28
ClaI AT'CG_AT
Cuts at: 0 10061 13281
Size: 10061 3220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 5787 5822 12117 12152 13281
Size: 5787 35 6295 35 1129
Fragments arranged by size:
6295 5787 1129 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 196 1464 2186 4994 6518 8664 11670 11970
Size: 196 1268 722 2808 1524 2146 3006 300
Cuts at: 11970 13281
Size: 1311
Fragments arranged by size:
3006 2808 2146 1524 1311 1268 722 300
196
DrdI GACnn_nn'nnGTC
Cuts at: 0 1014 8306 10609 13281
Size: 1014 7292 2303 2672
Fragments arranged by size:
7292 2672 2303 1014
EaeI y'GGCC_r
Cuts at: 0 36 89 1600 3254 3581 3747 4941 5730
Size: 36 53 1511 1654 327 166 1194 789
Cuts at: 5730 5855 6647 6881 7217 9558 12333 12470 12770
Size: 125 792 234 336 2341 2775 137 300
Cuts at: 12770 13281
Size: 511
Fragments arranged by size:
2775 2341 1654 1511 1194 792 789 511
336 327 300 234 166 137 125 53
36
EagI C'GGCC_G
Cuts at: 0 9558 13281
Size: 9558 3723
EarI CTCTTCn'nnn_
Cuts at: 0 241 1165 2327 2590 2958 3587 5993 6724
Size: 241 924 1162 263 368 629 2406 731
Cuts at: 6724 6997 7730 7843 8212 8549 8626 9260 9281
Size: 273 733 113 369 337 77 634 21
Cuts at: 9281 9803 12446 12668 13111 13281
Size: 522 2643 222 443 170
Fragments arranged by size:
2643 2406 1162 924 733 731 634 629
522 443 369 368 337 273 263 241
222 170 113 77 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 283 5257 5758 7495 8698 11755 13281
Size: 283 4974 501 1737 1203 3057 1526
Fragments arranged by size:
4974 3057 1737 1526 1203 501 283
EcoICRI GAG'CTC
Cuts at: 0 147 3678 8433 13281
Size: 147 3531 4755 4848
Fragments arranged by size:
4848 4755 3531 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 664 844 1045 1294 1630 3163 3181
Size: 391 273 180 201 249 336 1533 18
Cuts at: 3181 3223 3262 3361 3433 3643 3673 3688 4052
Size: 42 39 99 72 210 30 15 364
Cuts at: 4052 4648 5564 6032 6745 7287 7516 7705 8110
Size: 596 916 468 713 542 229 189 405
Cuts at: 8110 8155 8679 9103 9325 9526 9763 9827 9841
Size: 45 524 424 222 201 237 64 14
Cuts at: 9841 9847 9919 10015 10261 10303 10561 10609 10672
Size: 6 72 96 246 42 258 48 63
Cuts at: 10672 10789 10984 11503 12262 12455 12869 13281
Size: 117 195 519 759 193 414 412
Fragments arranged by size:
1533 916 759 713 596 542 524 519
468 424 414 412 405 391 364 336
273 258 249 246 237 229 222 210
201 201 195 193 189 180 117 99
96 72 72 64 63 48 45 42
42 39 30 18 15 14 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1485 2386 3670 5449 6193 8779 9286 9901
Size: 1485 901 1284 1779 744 2586 507 615
Cuts at: 9901 11386 11608 11644 12568 13281
Size: 1485 222 36 924 713
Fragments arranged by size:
2586 1779 1485 1485 1284 924 901 744
713 615 507 222 36
EcoO109I rG'GnC_Cy
Cuts at: 0 181 321 492 493 817 1219 1395 1998
Size: 181 140 171 1 324 402 176 603
Cuts at: 1998 2082 2319 3654 4726 4810 4866 5407 5442
Size: 84 237 1335 1072 84 56 541 35
Cuts at: 5442 5454 5493 6627 7246 7481 7594 8772 9300
Size: 12 39 1134 619 235 113 1178 528
Cuts at: 9300 9518 9894 9906 9964 10200 10236 10536 11619
Size: 218 376 12 58 236 36 300 1083
Cuts at: 11619 11853 12459 12996 13281
Size: 234 606 537 285
Fragments arranged by size:
1335 1178 1134 1083 1072 619 606 603
541 537 528 402 376 324 300 285
237 236 235 234 218 181 176 171
140 113 84 84 58 56 39 36
35 12 12 1
EcoRI G'AATT_C
Cuts at: 0 2641 3502 13281
Size: 2641 861 9779
Fragments arranged by size:
9779 2641 861
EcoRV GAT'ATC
Cuts at: 0 8400 9552 13281
Size: 8400 1152 3729
Fragments arranged by size:
8400 3729 1152
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 252 2931 3267 13281
Size: 252 2679 336 10014
Fragments arranged by size:
10014 2679 336 252
FspI TGC'GCA
Cuts at: 0 2005 10405 13281
Size: 2005 8400 2876
Fragments arranged by size:
8400 2876 2005
FspAI rTGC'GCAy
Cuts at: 0 10405 13281
Size: 10405 2876
HaeII r_GCGC'y
Cuts at: 0 949 2937 3138 5973 7134 7806 11589 12168
Size: 949 1988 201 2835 1161 672 3783 579
Cuts at: 12168 13124 13281
Size: 956 157
Fragments arranged by size:
3783 2835 1988 1161 956 949 672 579
201 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 615 647 1792 1797 1824 1829 3885 3917
Size: 615 32 1145 5 27 5 2056 32
Cuts at: 3917 3973 4005 4367 4399 4476 4508 5491 5523
Size: 56 32 362 32 77 32 983 32
Cuts at: 5523 5578 5610 6589 6621 7601 7633 7894 7926
Size: 55 32 979 32 980 32 261 32
Cuts at: 7926 8384 8416 8548 8580 9325 9357 9475 9507
Size: 458 32 132 32 745 32 118 32
Cuts at: 9507 10114 10146 10173 10205 11492 11524 11697 11729
Size: 607 32 27 32 1287 32 173 32
Cuts at: 11729 12054 12086 12343 12375 12904 12936 13171 13198
Size: 325 32 257 32 529 32 235 27
Cuts at: 13198 13203 13218 13230 13250 13281
Size: 5 15 12 20 31
Fragments arranged by size:
2056 1287 1145 983 980 979 745 615
607 529 458 362 325 261 257 235
173 132 118 77 56 55 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 31 27 27 27 20
15 12 5 5 5
HincII GTy'rAC
Cuts at: 0 1119 1893 4428 5481 6563 7179 7329 9999
Size: 1119 774 2535 1053 1082 616 150 2670
Cuts at: 9999 10515 13281
Size: 516 2766
Fragments arranged by size:
2766 2670 2535 1119 1082 1053 774 616
516 150
HindIII A'AGCT_T
Cuts at: 0 3996 5173 7717 13281
Size: 3996 1177 2544 5564
Fragments arranged by size:
5564 3996 2544 1177
KasI G'GCGC_C
Cuts at: 0 945 3134 12164 13281
Size: 945 2189 9030 1117
Fragments arranged by size:
9030 2189 1117 945
MfeI C'AATT_G
Cuts at: 0 5774 12684 13281
Size: 5774 6910 597
Fragments arranged by size:
6910 5774 597
MluI A'CGCG_T
Cuts at: 0 8640 13281
Size: 8640 4641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 1820 3405 4701 5147 7075 8885 9056
Size: 155 1665 1585 1296 446 1928 1810 171
Cuts at: 9056 10493 12059 13281
Size: 1437 1566 1222
Fragments arranged by size:
1928 1810 1665 1585 1566 1437 1296 1222
446 171 155
MscI TGG'CCA
Cuts at: 0 91 1602 3256 3583 4943 5857 6883 7219
Size: 91 1511 1654 327 1360 914 1026 336
Cuts at: 7219 12335 12772 13281
Size: 5116 437 509
Fragments arranged by size:
5116 1654 1511 1360 1026 914 509 437
336 327 91
MslI CAynn'nnrTG
Cuts at: 0 685 2390 3083 3577 4852 5587 5663 5710
Size: 685 1705 693 494 1275 735 76 47
Cuts at: 5710 5785 6244 6589 7441 7825 8455 8924 9145
Size: 75 459 345 852 384 630 469 221
Cuts at: 9145 9595 10564 10753 11069 11575 11830 12344 12595
Size: 450 969 189 316 506 255 514 251
Cuts at: 12595 12655 12778 12871 13281
Size: 60 123 93 410
Fragments arranged by size:
1705 1275 969 852 735 693 685 630
514 506 494 469 459 450 410 384
345 316 255 251 221 189 123 93
76 75 60 47
MspA1I CmG'CkG
Cuts at: 0 882 1199 2359 2828 3972 4395 5898 6263
Size: 882 317 1160 469 1144 423 1503 365
Cuts at: 6263 6662 6845 6910 8297 9943 10551 10713 10767
Size: 399 183 65 1387 1646 608 162 54
Cuts at: 10767 10836 11559 11736 11912 12475 13281
Size: 69 723 177 176 563 806
Fragments arranged by size:
1646 1503 1387 1160 1144 882 806 723
608 563 469 423 399 365 317 183
177 176 162 69 65 54
NaeI GCC'GGC
Cuts at: 0 5323 5643 12123 13281
Size: 5323 320 6480 1158
Fragments arranged by size:
6480 5323 1158 320
NarI GG'CG_CC
Cuts at: 0 946 3135 12165 13281
Size: 946 2189 9030 1116
Fragments arranged by size:
9030 2189 1116 946
NcoI C'CATG_G
Cuts at: 0 3776 5510 5780 6932 7589 8681 9605 13281
Size: 3776 1734 270 1152 657 1092 924 3676
Fragments arranged by size:
3776 3676 1734 1152 1092 924 657 270
NdeI CA'TA_TG
Cuts at: 0 11066 13281
Size: 11066 2215
NgoMIV G'CCGG_C
Cuts at: 0 5321 5641 12121 13281
Size: 5321 320 6480 1160
Fragments arranged by size:
6480 5321 1160 320
NheI G'CTAG_C
Cuts at: 0 12531 13281
Size: 12531 750
NruI TCG'CGA
Cuts at: 0 3106 6589 13281
Size: 3106 3483 6692
Fragments arranged by size:
6692 3483 3106
NsiI A_TGCA'T
Cuts at: 0 856 7832 13281
Size: 856 6976 5449
Fragments arranged by size:
6976 5449 856
NspI r_CATG'y
Cuts at: 0 390 1551 1884 2200 2883 3540 4042 6461
Size: 390 1161 333 316 683 657 502 2419
Cuts at: 6461 6768 7335 7476 7785 7878 8274 9594 12145
Size: 307 567 141 309 93 396 1320 2551
Cuts at: 12145 13134 13281
Size: 989 147
Fragments arranged by size:
2551 2419 1320 1161 989 683 657 567
502 396 390 333 316 309 307 147
141 93
PasI CC'CwG_GG
Cuts at: 0 1245 5457 7600 11604 13281
Size: 1245 4212 2143 4004 1677
Fragments arranged by size:
4212 4004 2143 1677 1245
PciI A'CATG_T
Cuts at: 0 2879 3536 7331 7472 7781 12141 13281
Size: 2879 657 3795 141 309 4360 1140
Fragments arranged by size:
4360 3795 2879 1140 657 309 141
PflMI CCAn_nnn'nTGG
Cuts at: 0 449 1044 1971 2255 2394 4121 4206 6419
Size: 449 595 927 284 139 1727 85 2213
Cuts at: 6419 6895 7310 8310 8747 9897 10554 12768 13281
Size: 476 415 1000 437 1150 657 2214 513
Fragments arranged by size:
2214 2213 1727 1150 1000 927 657 595
513 476 449 437 415 284 139 85
PfoI T'CCnGG_A
Cuts at: 0 974 1334 2108 3140 3338 3956 4505 4535
Size: 974 360 774 1032 198 618 549 30
Cuts at: 4535 10091 13281
Size: 5556 3190
Fragments arranged by size:
5556 3190 1032 974 774 618 549 360
198 30
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 820 852 4280 4312 6520 6552 13281
Size: 820 32 3428 32 2208 32 6729
Fragments arranged by size:
6729 3428 2208 820 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 181 321 817 1219 1395 1998 2319 3654
Size: 181 140 496 402 176 603 321 1335
Cuts at: 3654 4866 5442 6627 7246 7481 9906 9964 12459
Size: 1212 576 1185 619 235 2425 58 2495
Cuts at: 12459 13281
Size: 822
Fragments arranged by size:
2495 2425 1335 1212 1185 822 619 603
576 496 402 321 235 181 176 140
58
PspOMI G'GGCC_C
Cuts at: 0 492 4726 7593 9299 12809 13281
Size: 492 4234 2867 1706 3510 472
Fragments arranged by size:
4234 3510 2867 1706 492 472
PspXI vC'TCGA_Gb
Cuts at: 0 8779 13281
Size: 8779 4502
PstI C_TGCA'G
Cuts at: 0 140 675 2660 2858 3767 5415 7292 10259
Size: 140 535 1985 198 909 1648 1877 2967
Cuts at: 10259 11558 11741 11999 12572 12881 13104 13281
Size: 1299 183 258 573 309 223 177
Fragments arranged by size:
2967 1985 1877 1648 1299 909 573 535
309 258 223 198 183 177 140
PvuII CAG'CTG
Cuts at: 0 882 2359 4395 5898 6910 9943 10551 10713
Size: 882 1477 2036 1503 1012 3033 608 162
Cuts at: 10713 10767 11559 11736 13281
Size: 54 792 177 1545
Fragments arranged by size:
3033 2036 1545 1503 1477 1012 882 792
608 177 162 54
SacI G_AGCT'C
Cuts at: 0 149 3680 8435 13281
Size: 149 3531 4755 4846
Fragments arranged by size:
4846 4755 3531 149
SalI G'TCGA_C
Cuts at: 0 7177 13281
Size: 7177 6104
SanDI GG'GwC_CC
Cuts at: 0 1219 7246 7481 9964 13281
Size: 1219 6027 235 2483 3317
Fragments arranged by size:
6027 3317 2483 1219 235
SapI GCTCTTCn'nnn_
Cuts at: 0 1165 8212 13111 13281
Size: 1165 7047 4899 170
Fragments arranged by size:
7047 4899 1165 170
SbfI CC_TGCA'GG
Cuts at: 0 5415 13281
Size: 5415 7866
ScaI AGT'ACT
Cuts at: 0 5305 13281
Size: 5305 7976
SexAI A'CCwGG_T
Cuts at: 0 3950 4217 4421 5054 7199 9134 9917 13281
Size: 3950 267 204 633 2145 1935 783 3364
Fragments arranged by size:
3950 3364 2145 1935 783 633 267 204
SfcI C'TryA_G
Cuts at: 0 136 326 671 2656 2854 3763 4088 5411
Size: 136 190 345 1985 198 909 325 1323
Cuts at: 5411 5977 6289 7288 10255 10373 10479 11010 11554
Size: 566 312 999 2967 118 106 531 544
Cuts at: 11554 11737 11995 12568 12877 13100 13281
Size: 183 258 573 309 223 181
Fragments arranged by size:
2967 1985 1323 999 909 573 566 544
531 345 325 312 309 258 223 198
190 183 181 136 118 106
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 6646 9298 12808 13281
Size: 6646 2652 3510 473
Fragments arranged by size:
6646 3510 2652 473
SfoI GGC'GCC
Cuts at: 0 947 3136 12166 13281
Size: 947 2189 9030 1115
Fragments arranged by size:
9030 2189 1115 947
SmaI CCC'GGG
Cuts at: 0 2129 5158 8587 12448 13281
Size: 2129 3029 3429 3861 833
Fragments arranged by size:
3861 3429 3029 2129 833
SmlI C'TyrA_G
Cuts at: 0 185 2134 2866 3058 5035 8434 8779 10642
Size: 185 1949 732 192 1977 3399 345 1863
Cuts at: 10642 12106 12463 13281
Size: 1464 357 818
Fragments arranged by size:
3399 1977 1949 1863 1464 818 732 357
345 192 185
SphI G_CATG'C
Cuts at: 0 390 1884 7878 13281
Size: 390 1494 5994 5403
Fragments arranged by size:
5994 5403 1494 390
StuI AGG'CCT
Cuts at: 0 3443 4261 4502 6295 12328 13281
Size: 3443 818 241 1793 6033 953
Fragments arranged by size:
6033 3443 1793 953 818 241
StyI C'CwwG_G
Cuts at: 0 152 1061 2543 3650 3776 4004 4371 5510
Size: 152 909 1482 1107 126 228 367 1139
Cuts at: 5510 5780 6194 6365 6378 6932 7397 7589 7627
Size: 270 414 171 13 554 465 192 38
Cuts at: 7627 7859 8099 8681 8723 9605 9668 10107 11433
Size: 232 240 582 42 882 63 439 1326
Cuts at: 11433 12094 12260 12974 13124 13281
Size: 661 166 714 150 157
Fragments arranged by size:
1482 1326 1139 1107 909 882 714 661
582 554 465 439 414 367 270 240
232 228 192 171 166 157 152 150
126 63 42 38 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1638 2664 3854 4504 4511 4914 5443 5665
Size: 1638 1026 1190 650 7 403 529 222
Cuts at: 5665 8479 8949 9585 11658 11807 12438 13196 13281
Size: 2814 470 636 2073 149 631 758 85
Fragments arranged by size:
2814 2073 1638 1190 1026 758 650 636
631 529 470 403 222 149 85 7
TatI w'GTAC_w
Cuts at: 0 393 2361 2468 4352 4775 5303 5522 5867
Size: 393 1968 107 1884 423 528 219 345
Cuts at: 5867 6839 6869 7559 7784 13133 13281
Size: 972 30 690 225 5349 148
Fragments arranged by size:
5349 1968 1884 972 690 528 423 393
345 225 219 148 107 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 262 294 895 927 956 988 2686 2718
Size: 262 32 601 32 29 32 1698 32
Cuts at: 2718 5727 5759 6444 6476 6502 6534 9211 9243
Size: 3009 32 685 32 26 32 2677 32
Cuts at: 9243 10295 10327 11584 11616 11654 11686 13281
Size: 1052 32 1257 32 38 32 1595
Fragments arranged by size:
3009 2677 1698 1595 1257 1052 685 601
262 38 32 32 32 32 32 32
32 32 32 32 32 29 26
Tth111I GACn'n_nGTC
Cuts at: 0 1678 3334 3952 4423 8442 13281
Size: 1678 1656 618 471 4019 4839
Fragments arranged by size:
4839 4019 1678 1656 618 471
XbaI T'CTAG_A
Cuts at: 0 9261 13281
Size: 9261 4020
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1776 5920 6243 7239 7755 8943 9099 9300
Size: 1776 4144 323 996 516 1188 156 201
Cuts at: 9300 11180 12351 12752 13281
Size: 1880 1171 401 529
Fragments arranged by size:
4144 1880 1776 1188 1171 996 529 516
401 323 201 156
XhoI C'TCGA_G
Cuts at: 0 8779 13281
Size: 8779 4502
XmaI C'CCGG_G
Cuts at: 0 2127 5156 8585 12446 13281
Size: 2127 3029 3429 3861 835
Fragments arranged by size:
3861 3429 3029 2127 835
XmnI GAAnn'nnTTC
Cuts at: 0 2227 3100 4366 4544 5816 6002 13281
Size: 2227 873 1266 178 1272 186 7279
Fragments arranged by size:
7279 2227 1272 1266 873 186 178
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI FspAI HaeII Hin4I HincII HindIII KasI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NruI NsiI NspI PasI PciI
PflMI PfoI PpiI PpuMI PspOMI PspXI PstI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SmaI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AscI AseI AsiSI BplI DraI FseI
HpaI KpnI NotI PacI PmeI PmlI PshAI PsiI
PsrI PvuI RsrII SacII SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI ZraI