Restriction map for
pF1KB0499
(Linear) (Six-base) MAPSORT of: KIBB0499.seq Check: 6057 from: 1 to: 2262
KIBB0499.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AatII G_ACGT'C
Cuts at: 0 507 2262
Size: 507 1755
AccI GT'mk_AC
Cuts at: 0 1384 2262
Size: 1384 878
Acc65I G'GTAC_C
Cuts at: 0 223 2262
Size: 223 2039
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 938 1912 2262
Size: 938 974 350
Fragments arranged by size:
974 938 350
AflIII A'CryG_T
Cuts at: 0 802 2262
Size: 802 1460
ApaI G_GGCC'C
Cuts at: 0 28 2262
Size: 28 2234
ApaLI G'TGCA_C
Cuts at: 0 488 2262
Size: 488 1774
ApoI r'AATT_y
Cuts at: 0 313 535 1174 1599 1972 2049 2059 2262
Size: 313 222 639 425 373 77 10 203
Fragments arranged by size:
639 425 373 313 222 203 77 10
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 215 248 2262
Size: 215 33 2014
Fragments arranged by size:
2014 215 33
BanI G'GyrC_C
Cuts at: 0 223 2262
Size: 223 2039
BanII G_rGCy'C
Cuts at: 0 28 2262
Size: 28 2234
BbsI GAAGACnn'nnnn_
Cuts at: 0 1418 1889 2262
Size: 1418 471 373
Fragments arranged by size:
1418 471 373
BclI T'GATC_A
Cuts at: 0 111 2262
Size: 111 2151
BglII A'GATC_T
Cuts at: 0 2118 2262
Size: 2118 144
Bme1580I G_kGCm'C
Cuts at: 0 28 492 2262
Size: 28 464 1770
Fragments arranged by size:
1770 464 28
BmrI ACTGGGnnnn_n'
Cuts at: 0 980 1063 2262
Size: 980 83 1199
Fragments arranged by size:
1199 980 83
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 995 2102 2262
Size: 995 1107 160
Fragments arranged by size:
1107 995 160
Bpu10I CC'TnA_GC
Cuts at: 0 2167 2262
Size: 2167 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1829 1861 2262
Size: 1829 32 401
Fragments arranged by size:
1829 401 32
BsaAI yAC'GTr
Cuts at: 0 1089 2262
Size: 1089 1173
BsaBI GATnn'nnATC
Cuts at: 0 1273 2262
Size: 1273 989
BsaHI Gr'CG_yC
Cuts at: 0 504 2262
Size: 504 1758
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 993 1023 2262
Size: 993 30 1239
Fragments arranged by size:
1239 993 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 50 2262
Size: 50 2212
BsiHKAI G_wGCw'C
Cuts at: 0 492 2262
Size: 492 1770
BsmI GAATG_Cn'
Cuts at: 0 442 696 1187 2262
Size: 442 254 491 1075
Fragments arranged by size:
1075 491 442 254
Bsp1286I G_dGCh'C
Cuts at: 0 28 492 2262
Size: 28 464 1770
Fragments arranged by size:
1770 464 28
BspHI T'CATG_A
Cuts at: 0 947 2262
Size: 947 1315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1491 2262
Size: 1491 771
BstYI r'GATC_y
Cuts at: 0 2118 2262
Size: 2118 144
Bsu36I CC'TnA_GG
Cuts at: 0 974 2262
Size: 974 1288
BtgI C'CryG_G
Cuts at: 0 20 2262
Size: 20 2242
DraI TTT'AAA
Cuts at: 0 383 1583 2249 2262
Size: 383 1200 666 13
Fragments arranged by size:
1200 666 383 13
EaeI y'GGCC_r
Cuts at: 0 1667 2262
Size: 1667 595
EarI CTCTTCn'nnn_
Cuts at: 0 354 755 2153 2201 2262
Size: 354 401 1398 48 61
Fragments arranged by size:
1398 401 354 61 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 938 995 1912 2102 2262
Size: 938 57 917 190 160
Fragments arranged by size:
938 917 190 160 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2084 2262
Size: 2084 178
EcoRI G'AATT_C
Cuts at: 0 535 2262
Size: 535 1727
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2230 2262
Size: 2230 32
HaeII r_GCGC'y
Cuts at: 0 1067 2262
Size: 1067 1195
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 592 624 993 1025 1087 1119 1534 1566
Size: 592 32 369 32 62 32 415 32
Cuts at: 1566 1570 1602 2262
Size: 4 32 660
Fragments arranged by size:
660 592 415 369 62 32 32 32
32 32 4
HincII GTy'rAC
Cuts at: 0 143 2041 2262
Size: 143 1898 221
Fragments arranged by size:
1898 221 143
HpaI GTT'AAC
Cuts at: 0 143 2262
Size: 143 2119
KpnI G_GTAC'C
Cuts at: 0 227 2262
Size: 227 2035
MfeI C'AATT_G
Cuts at: 0 423 509 2262
Size: 423 86 1753
Fragments arranged by size:
1753 423 86
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 2262
Size: 57 2205
MscI TGG'CCA
Cuts at: 0 1669 2262
Size: 1669 593
MspA1I CmG'CkG
Cuts at: 0 22 2262
Size: 22 2240
NspI r_CATG'y
Cuts at: 0 347 2200 2262
Size: 347 1853 62
Fragments arranged by size:
1853 347 62
PflMI CCAn_nnn'nTGG
Cuts at: 0 613 2262
Size: 613 1649
PfoI T'CCnGG_A
Cuts at: 0 795 1011 2079 2262
Size: 795 216 1068 183
Fragments arranged by size:
1068 795 216 183
PspOMI G'GGCC_C
Cuts at: 0 24 2262
Size: 24 2238
PstI C_TGCA'G
Cuts at: 0 1626 2262
Size: 1626 636
SacII CC_GC'GG
Cuts at: 0 23 2262
Size: 23 2239
SapI GCTCTTCn'nnn_
Cuts at: 0 354 2262
Size: 354 1908
SfcI C'TryA_G
Cuts at: 0 1622 2262
Size: 1622 640
SmlI C'TyrA_G
Cuts at: 0 1844 1876 2262
Size: 1844 32 386
Fragments arranged by size:
1844 386 32
SnaBI TAC'GTA
Cuts at: 0 1089 2262
Size: 1089 1173
SphI G_CATG'C
Cuts at: 0 347 2262
Size: 347 1915
SspI AAT'ATT
Cuts at: 0 526 2262
Size: 526 1736
StyI C'CwwG_G
Cuts at: 0 1777 2262
Size: 1777 485
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1215 2262
Size: 1215 1047
TatI w'GTAC_w
Cuts at: 0 988 2262
Size: 988 1274
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 75 107 923 955 1854 1886 2262
Size: 75 32 816 32 899 32 376
Fragments arranged by size:
899 816 376 75 32 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 1185 2262
Size: 1185 1077
ZraI GAC'GTC
Cuts at: 0 505 2262
Size: 505 1757
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII ApaI ApaLI ApoI
BaeI BanI BanII BbsI BclI BglII Bme1580I BmrI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI
BsiHKAI BsmI Bsp1286I BspHI BspMI BstYI Bsu36I BtgI
DraI EaeI EarI Eco57MI EcoNI EcoRI FalI HaeII
Hin4I HincII HpaI KpnI MfeI MmeI MscI MspA1I
NspI PflMI PfoI PspOMI PstI SacII SapI SfcI
SmlI SnaBI SphI SspI StyI TaqII TatI TstI
XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AhdI AleI AlfI
AloI AlwNI AscI AseI AsiSI AvaI AvrII BamHI
BbeI BbvCI BcgI BciVI BfrBI BglI BlpI BmgBI
BmtI BplI BsaI BsaWI BseYI BsgI BsiEI BsiWI
BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI
CspCI DraIII DrdI EagI EciI EcoICRI EcoO109I EcoRV
FseI FspI FspAI HindIII KasI MluI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SpeI SrfI StuI SwaI Tth111I XbaI XcmI
XhoI XmaI