(Linear) (Six-base) MAPSORT of: KIBB0901.seq  Check: 7723  from: 1  to: 240



KIBB0901.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     240 
   Size:        200      40
 
AhdI GACnn_n'nnGTC

Cuts at:      0     168     240 
   Size:        168      72
 
AlwNI CAG_nnn'CTG

Cuts at:      0     223     240 
   Size:        223      17
 
BanII G_rGCy'C

Cuts at:      0     181     240 
   Size:        181      59
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     130     240 
   Size:        130     110
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     228     240 
   Size:        228      12
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     240 
   Size:        150      90
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      15      45     240 
   Size:         15      30     195

  Fragments arranged by size: 

                 195      30      15
 
BseYI C'CCAG_C

Cuts at:      0     114     240 
   Size:        114     126
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     171     240 
   Size:         94      77      69

  Fragments arranged by size: 

                  94      77      69
 
Bsp1286I G_dGCh'C

Cuts at:      0     181     240 
   Size:        181      59
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     193     240 
   Size:        193      47
 
Bsu36I CC'TnA_GG

Cuts at:      0      35     240 
   Size:         35     205
 
EarI CTCTTCn'nnn_

Cuts at:      0     219     240 
   Size:        219      21
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     175     240 
   Size:        175      65
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     200     240 
   Size:        150      50      40

  Fragments arranged by size: 

                 150      50      40
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     154     186     240 
   Size:        154      32      54

  Fragments arranged by size: 

                 154      54      32
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      15      47     240 
   Size:         15      32     193

  Fragments arranged by size: 

                 193      32      15
 

 Enzymes that do cut:

     AcuI     AhdI    AlwNI    BanII    BciVI     BplI     BpmI    BsaXI
    BseYI     BsgI Bsp1286I    BspMI   Bsu36I     EarI     EciI  Eco57MI
    Hin4I     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI