Restriction map for
pF1KB0902
(Linear) (Six-base) MAPSORT of: KIBB0902.seq Check: 7486 from: 1 to: 891
KIBB0902.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 553 891
Size: 553 338
AccI GT'mk_AC
Cuts at: 0 350 440 891
Size: 350 90 451
Fragments arranged by size:
451 350 90
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 832 891
Size: 25 807 59
Fragments arranged by size:
807 59 25
AflIII A'CryG_T
Cuts at: 0 571 891
Size: 571 320
AhdI GACnn_n'nnGTC
Cuts at: 0 90 891
Size: 90 801
AvrII C'CTAG_G
Cuts at: 0 405 891
Size: 405 486
BciVI GTATCCnnnnn_n'
Cuts at: 0 506 891
Size: 506 385
BclI T'GATC_A
Cuts at: 0 666 891
Size: 666 225
Bme1580I G_kGCm'C
Cuts at: 0 110 637 891
Size: 110 527 254
Fragments arranged by size:
527 254 110
BmgBI CAC'GTC
Cuts at: 0 574 891
Size: 574 317
BmrI ACTGGGnnnn_n'
Cuts at: 0 546 891
Size: 546 345
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 435 891
Size: 435 456
BsaI GGTCTCn'nnnn_
Cuts at: 0 411 891
Size: 411 480
BsaWI w'CCGG_w
Cuts at: 0 656 891
Size: 656 235
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 187 205 208 211 382 805 891
Size: 172 15 18 3 3 171 423 86
Fragments arranged by size:
423 172 171 86 18 15 3 3
BseYI C'CCAG_C
Cuts at: 0 22 134 891
Size: 22 112 757
Fragments arranged by size:
757 112 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 891
Size: 820 71
BsmI GAATG_Cn'
Cuts at: 0 273 866 891
Size: 273 593 25
Fragments arranged by size:
593 273 25
Bsp1286I G_dGCh'C
Cuts at: 0 110 637 891
Size: 110 527 254
Fragments arranged by size:
527 254 110
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 553 891
Size: 553 338
BstAPI GCAn_nnn'nTGC
Cuts at: 0 777 891
Size: 777 114
BstXI CCAn_nnnn'nTGG
Cuts at: 0 69 738 891
Size: 69 669 153
Fragments arranged by size:
669 153 69
BtgI C'CryG_G
Cuts at: 0 221 891
Size: 221 670
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 818 853 891
Size: 818 35 38
Fragments arranged by size:
818 38 35
DraI TTT'AAA
Cuts at: 0 684 891
Size: 684 207
DraIII CAC_nnn'GTG
Cuts at: 0 777 891
Size: 777 114
EaeI y'GGCC_r
Cuts at: 0 602 891
Size: 602 289
EarI CTCTTCn'nnn_
Cuts at: 0 272 377 482 712 891
Size: 272 105 105 230 179
Fragments arranged by size:
272 230 179 105 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 832 891
Size: 25 807 59
Fragments arranged by size:
807 59 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 43 891
Size: 43 848
EcoO109I rG'GnC_Cy
Cuts at: 0 84 891
Size: 84 807
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 651 683 891
Size: 76 32 543 32 208
Fragments arranged by size:
543 208 76 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 803 891
Size: 803 88
MscI TGG'CCA
Cuts at: 0 604 891
Size: 604 287
MslI CAynn'nnrTG
Cuts at: 0 736 891
Size: 736 155
MspA1I CmG'CkG
Cuts at: 0 101 138 600 891
Size: 101 37 462 291
Fragments arranged by size:
462 291 101 37
NcoI C'CATG_G
Cuts at: 0 221 891
Size: 221 670
NspI r_CATG'y
Cuts at: 0 678 891
Size: 678 213
PfoI T'CCnGG_A
Cuts at: 0 754 891
Size: 754 137
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 299 331 891
Size: 299 32 560
Fragments arranged by size:
560 299 32
PpuMI rG'GwC_Cy
Cuts at: 0 84 891
Size: 84 807
PvuII CAG'CTG
Cuts at: 0 101 138 600 891
Size: 101 37 462 291
Fragments arranged by size:
462 291 101 37
SapI GCTCTTCn'nnn_
Cuts at: 0 482 891
Size: 482 409
SmlI C'TyrA_G
Cuts at: 0 414 891
Size: 414 477
StuI AGG'CCT
Cuts at: 0 880 891
Size: 880 11
StyI C'CwwG_G
Cuts at: 0 221 405 891
Size: 221 184 486
Fragments arranged by size:
486 221 184
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 40 72 891
Size: 40 32 819
Fragments arranged by size:
819 40 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 597 891
Size: 597 294
XmnI GAAnn'nnTTC
Cuts at: 0 706 866 891
Size: 706 160 25
Fragments arranged by size:
706 160 25
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AvrII BciVI BclI
Bme1580I BmgBI BmrI BpuEI BsaI BsaWI BseRI BseYI
BsgI BsmI Bsp1286I BspMI BstAPI BstXI BtgI CspCI
DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I Hin4I
MmeI MscI MslI MspA1I NcoI NspI PfoI PpiI
PpuMI PvuII SapI SmlI StuI StyI TstI XcmI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BfrBI BglI BglII BlpI BmtI BplI BpmI
Bpu10I BsaAI BsaBI BsaHI BsaXI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI DrdI EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI Tth111I XbaI
XhoI XmaI ZraI