(Linear) (Six-base) MAPSORT of: KIBB0903.seq  Check: 1422  from: 1  to: 270



KIBB0903.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

ApoI r'AATT_y

Cuts at:      0      32     211     270 
   Size:         32     179      59

  Fragments arranged by size: 

                 179      59      32
 
AvaI C'yCGr_G

Cuts at:      0     161     270 
   Size:        161     109
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     119     242     270 
   Size:        119     123      28

  Fragments arranged by size: 

                 123     119      28
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      74     270 
   Size:         74     196
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      88     270 
   Size:         88     182
 
EcoO109I rG'GnC_Cy

Cuts at:      0     193     270 
   Size:        193      77
 
MslI CAynn'nnrTG

Cuts at:      0     148     227     270 
   Size:        148      79      43

  Fragments arranged by size: 

                 148      79      43
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      48     270 
   Size:         48     222
 
PpuMI rG'GwC_Cy

Cuts at:      0     193     270 
   Size:        193      77
 
PshAI GACnn'nnGTC

Cuts at:      0     198     270 
   Size:        198      72
 
PspXI vC'TCGA_Gb

Cuts at:      0     161     270 
   Size:        161     109
 
SmlI C'TyrA_G

Cuts at:      0     161     270 
   Size:        161     109
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      40     270 
   Size:         40     230
 
Tth111I GACn'n_nGTC

Cuts at:      0     145     270 
   Size:        145     125
 
XhoI C'TCGA_G

Cuts at:      0     161     270 
   Size:        161     109
 

 Enzymes that do cut:

     ApoI     AvaI     BbsI     BsgI    EcoNI EcoO109I     MslI    PflMI
    PpuMI    PshAI    PspXI     SmlI    TaqII  Tth111I     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI     PsiI   PspOMI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI
     XbaI     XcmI     XmaI     XmnI     ZraI