Restriction map for
pF1KB0904
(Linear) (Six-base) MAPSORT of: KIBB0904.seq Check: 8403 from: 1 to: 897
KIBB0904.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 192 507 897
Size: 88 104 315 390
Fragments arranged by size:
390 315 104 88
AlwNI CAG_nnn'CTG
Cuts at: 0 586 897
Size: 586 311
ApaI G_GGCC'C
Cuts at: 0 47 474 630 897
Size: 47 427 156 267
Fragments arranged by size:
427 267 156 47
AvaI C'yCGr_G
Cuts at: 0 47 897
Size: 47 850
AvrII C'CTAG_G
Cuts at: 0 369 897
Size: 369 528
BamHI G'GATC_C
Cuts at: 0 212 897
Size: 212 685
BanI G'GyrC_C
Cuts at: 0 88 897
Size: 88 809
BanII G_rGCy'C
Cuts at: 0 47 474 630 897
Size: 47 427 156 267
Fragments arranged by size:
427 267 156 47
BbeI G_GCGC'C
Cuts at: 0 92 897
Size: 92 805
BciVI GTATCCnnnnn_n'
Cuts at: 0 489 897
Size: 489 408
BclI T'GATC_A
Cuts at: 0 458 897
Size: 458 439
BglI GCCn_nnn'nGGC
Cuts at: 0 42 625 897
Size: 42 583 272
Fragments arranged by size:
583 272 42
BlpI GC'TnA_GC
Cuts at: 0 559 897
Size: 559 338
Bme1580I G_kGCm'C
Cuts at: 0 47 61 140 474 630 897
Size: 47 14 79 334 156 267
Fragments arranged by size:
334 267 156 79 47 14
Bpu10I CC'TnA_GC
Cuts at: 0 341 897
Size: 341 556
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 897
Size: 406 491
BsaHI Gr'CG_yC
Cuts at: 0 89 897
Size: 89 808
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 261 897
Size: 261 636
BseYI C'CCAG_C
Cuts at: 0 78 464 608 620 897
Size: 78 386 144 12 277
Fragments arranged by size:
386 277 144 78 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 697 897
Size: 193 504 200
Fragments arranged by size:
504 200 193
BsiEI CG_ry'CG
Cuts at: 0 791 897
Size: 791 106
BsiHKAI G_wGCw'C
Cuts at: 0 422 897
Size: 422 475
Bsp1286I G_dGCh'C
Cuts at: 0 47 61 140 422 474 630 897
Size: 47 14 79 282 52 156 267
Fragments arranged by size:
282 267 156 79 52 47 14
BsrFI r'CCGG_y
Cuts at: 0 85 897
Size: 85 812
BstXI CCAn_nnnn'nTGG
Cuts at: 0 603 897
Size: 603 294
BstYI r'GATC_y
Cuts at: 0 212 897
Size: 212 685
BtgI C'CryG_G
Cuts at: 0 92 125 897
Size: 92 33 772
Fragments arranged by size:
772 92 33
EaeI y'GGCC_r
Cuts at: 0 191 434 545 897
Size: 191 243 111 352
Fragments arranged by size:
352 243 191 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 481 897
Size: 481 416
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 192 507 897
Size: 88 104 315 390
Fragments arranged by size:
390 315 104 88
EcoNI CCTnn'n_nnAGG
Cuts at: 0 193 883 897
Size: 193 690 14
Fragments arranged by size:
690 193 14
EcoO109I rG'GnC_Cy
Cuts at: 0 44 198 897
Size: 44 154 699
Fragments arranged by size:
699 154 44
HaeII r_GCGC'y
Cuts at: 0 92 369 897
Size: 92 277 528
Fragments arranged by size:
528 277 92
HincII GTy'rAC
Cuts at: 0 411 897
Size: 411 486
KasI G'GCGC_C
Cuts at: 0 88 897
Size: 88 809
MscI TGG'CCA
Cuts at: 0 436 547 897
Size: 436 111 350
Fragments arranged by size:
436 350 111
MspA1I CmG'CkG
Cuts at: 0 456 468 583 624 663 823 897
Size: 456 12 115 41 39 160 74
Fragments arranged by size:
456 160 115 74 41 39 12
NaeI GCC'GGC
Cuts at: 0 87 897
Size: 87 810
NarI GG'CG_CC
Cuts at: 0 89 897
Size: 89 808
NgoMIV G'CCGG_C
Cuts at: 0 85 897
Size: 85 812
NspI r_CATG'y
Cuts at: 0 360 897
Size: 360 537
PflMI CCAn_nnn'nTGG
Cuts at: 0 636 897
Size: 636 261
PfoI T'CCnGG_A
Cuts at: 0 533 897
Size: 533 364
PspOMI G'GGCC_C
Cuts at: 0 43 470 626 897
Size: 43 427 156 271
Fragments arranged by size:
427 271 156 43
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 724 756 897
Size: 724 32 141
Fragments arranged by size:
724 141 32
PvuI CG_AT'CG
Cuts at: 0 791 897
Size: 791 106
PvuII CAG'CTG
Cuts at: 0 456 583 823 897
Size: 456 127 240 74
Fragments arranged by size:
456 240 127 74
SfcI C'TryA_G
Cuts at: 0 278 295 587 844 897
Size: 278 17 292 257 53
Fragments arranged by size:
292 278 257 53 17
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 625 897
Size: 625 272
SfoI GGC'GCC
Cuts at: 0 90 897
Size: 90 807
SmaI CCC'GGG
Cuts at: 0 49 897
Size: 49 848
SmlI C'TyrA_G
Cuts at: 0 421 897
Size: 421 476
StyI C'CwwG_G
Cuts at: 0 217 369 515 897
Size: 217 152 146 382
Fragments arranged by size:
382 217 152 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 478 897
Size: 478 419
TatI w'GTAC_w
Cuts at: 0 575 897
Size: 575 322
XmaI C'CCGG_G
Cuts at: 0 47 897
Size: 47 850
Enzymes that do cut:
AcuI AlwNI ApaI AvaI AvrII BamHI BanI BanII
BbeI BciVI BclI BglI BlpI Bme1580I Bpu10I BpuEI
BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrFI
BstXI BstYI BtgI EaeI EciI Eco57MI EcoNI EcoO109I
HaeII HincII KasI MscI MspA1I NaeI NarI NgoMIV
NspI PflMI PfoI PspOMI PsrI PvuI PvuII SfcI
SfiI SfoI SmaI SmlI StyI TaqII TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI BaeI BbsI BbvCI BcgI BfrBI BglII
BmgBI BmrI BmtI BplI BpmI BsaI BsaAI BsaBI
BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EarI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HindIII HpaI KpnI MfeI MluI
MmeI MslI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PstI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XcmI XhoI XmnI ZraI