Restriction map for
pF1KB0906
(Linear) (Six-base) MAPSORT of: KIBB0906.seq Check: 5519 from: 1 to: 915
KIBB0906.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 571 915
Size: 304 267 344
Fragments arranged by size:
344 304 267
AlwNI CAG_nnn'CTG
Cuts at: 0 192 276 637 915
Size: 192 84 361 278
Fragments arranged by size:
361 278 192 84
ApaLI G'TGCA_C
Cuts at: 0 139 915
Size: 139 776
ApoI r'AATT_y
Cuts at: 0 481 915
Size: 481 434
AvaI C'yCGr_G
Cuts at: 0 43 89 915
Size: 43 46 826
Fragments arranged by size:
826 46 43
BamHI G'GATC_C
Cuts at: 0 266 866 915
Size: 266 600 49
Fragments arranged by size:
600 266 49
BanI G'GyrC_C
Cuts at: 0 93 226 424 915
Size: 93 133 198 491
Fragments arranged by size:
491 198 133 93
BanII G_rGCy'C
Cuts at: 0 44 550 915
Size: 44 506 365
Fragments arranged by size:
506 365 44
BbeI G_GCGC'C
Cuts at: 0 97 915
Size: 97 818
BbvCI CC'TCA_GC
Cuts at: 0 530 915
Size: 530 385
Bme1580I G_kGCm'C
Cuts at: 0 143 229 915
Size: 143 86 686
Fragments arranged by size:
686 143 86
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 184 382 422 915
Size: 133 51 198 40 493
Fragments arranged by size:
493 198 133 51 40
Bpu10I CC'TnA_GC
Cuts at: 0 530 915
Size: 530 385
BsaI GGTCTCn'nnnn_
Cuts at: 0 160 915
Size: 160 755
BsaAI yAC'GTr
Cuts at: 0 489 915
Size: 489 426
BsaHI Gr'CG_yC
Cuts at: 0 94 915
Size: 94 821
BsaWI w'CCGG_w
Cuts at: 0 869 915
Size: 869 46
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 229 259 915
Size: 229 30 656
Fragments arranged by size:
656 229 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 547 580 655 915
Size: 130 417 33 75 260
Fragments arranged by size:
417 260 130 75 33
BseYI C'CCAG_C
Cuts at: 0 441 851 915
Size: 441 410 64
Fragments arranged by size:
441 410 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 814 915
Size: 511 303 101
Fragments arranged by size:
511 303 101
BsiEI CG_ry'CG
Cuts at: 0 101 125 915
Size: 101 24 790
Fragments arranged by size:
790 101 24
BsiHKAI G_wGCw'C
Cuts at: 0 143 250 915
Size: 143 107 665
Fragments arranged by size:
665 143 107
BsmI GAATG_Cn'
Cuts at: 0 371 915
Size: 371 544
Bsp1286I G_dGCh'C
Cuts at: 0 44 143 229 250 550 915
Size: 44 99 86 21 300 365
Fragments arranged by size:
365 300 99 86 44 21
BspEI T'CCGG_A
Cuts at: 0 869 915
Size: 869 46
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 178 915
Size: 178 737
BsrBI CCG'CTC
Cuts at: 0 177 570 915
Size: 177 393 345
Fragments arranged by size:
393 345 177
BsrFI r'CCGG_y
Cuts at: 0 69 96 915
Size: 69 27 819
Fragments arranged by size:
819 69 27
BssHII G'CGCG_C
Cuts at: 0 75 915
Size: 75 840
BstAPI GCAn_nnn'nTGC
Cuts at: 0 498 915
Size: 498 417
BstXI CCAn_nnnn'nTGG
Cuts at: 0 360 915
Size: 360 555
BstYI r'GATC_y
Cuts at: 0 266 866 915
Size: 266 600 49
Fragments arranged by size:
600 266 49
Bsu36I CC'TnA_GG
Cuts at: 0 34 915
Size: 34 881
BtgI C'CryG_G
Cuts at: 0 101 206 221 915
Size: 101 105 15 694
Fragments arranged by size:
694 105 101 15
DraIII CAC_nnn'GTG
Cuts at: 0 207 915
Size: 207 708
EaeI y'GGCC_r
Cuts at: 0 98 122 194 915
Size: 98 24 72 721
Fragments arranged by size:
721 98 72 24
EagI C'GGCC_G
Cuts at: 0 98 122 915
Size: 98 24 793
Fragments arranged by size:
793 98 24
EarI CTCTTCn'nnn_
Cuts at: 0 862 915
Size: 862 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 184 304 382 422 571 915
Size: 133 51 120 78 40 149 344
Fragments arranged by size:
344 149 133 120 78 51 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 112 915
Size: 112 803
EcoO109I rG'GnC_Cy
Cuts at: 0 49 273 315 333 915
Size: 49 224 42 18 582
Fragments arranged by size:
582 224 49 42 18
FspI TGC'GCA
Cuts at: 0 58 915
Size: 58 857
HaeII r_GCGC'y
Cuts at: 0 97 163 915
Size: 97 66 752
Fragments arranged by size:
752 97 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 771 792 803 824 915
Size: 771 21 11 21 91
Fragments arranged by size:
771 91 21 21 11
KasI G'GCGC_C
Cuts at: 0 93 915
Size: 93 822
MscI TGG'CCA
Cuts at: 0 196 915
Size: 196 719
MspA1I CmG'CkG
Cuts at: 0 223 495 863 915
Size: 223 272 368 52
Fragments arranged by size:
368 272 223 52
NaeI GCC'GGC
Cuts at: 0 98 915
Size: 98 817
NarI GG'CG_CC
Cuts at: 0 94 915
Size: 94 821
NgoMIV G'CCGG_C
Cuts at: 0 96 915
Size: 96 819
PflMI CCAn_nnn'nTGG
Cuts at: 0 207 276 466 915
Size: 207 69 190 449
Fragments arranged by size:
449 207 190 69
PfoI T'CCnGG_A
Cuts at: 0 359 915
Size: 359 556
PmlI CAC'GTG
Cuts at: 0 489 915
Size: 489 426
PpuMI rG'GwC_Cy
Cuts at: 0 49 315 333 915
Size: 49 266 18 582
Fragments arranged by size:
582 266 49 18
PstI C_TGCA'G
Cuts at: 0 27 555 915
Size: 27 528 360
Fragments arranged by size:
528 360 27
SacII CC_GC'GG
Cuts at: 0 224 915
Size: 224 691
SanDI GG'GwC_CC
Cuts at: 0 49 915
Size: 49 866
SapI GCTCTTCn'nnn_
Cuts at: 0 862 915
Size: 862 53
SexAI A'CCwGG_T
Cuts at: 0 329 915
Size: 329 586
SfcI C'TryA_G
Cuts at: 0 23 551 915
Size: 23 528 364
Fragments arranged by size:
528 364 23
SfoI GGC'GCC
Cuts at: 0 95 915
Size: 95 820
SmaI CCC'GGG
Cuts at: 0 91 915
Size: 91 824
SrfI GCCC'GGGC
Cuts at: 0 91 915
Size: 91 824
StyI C'CwwG_G
Cuts at: 0 473 915
Size: 473 442
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 412 915
Size: 412 503
TatI w'GTAC_w
Cuts at: 0 582 915
Size: 582 333
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 229 261 915
Size: 229 32 654
Fragments arranged by size:
654 229 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 204 915
Size: 204 711
XmaI C'CCGG_G
Cuts at: 0 89 915
Size: 89 826
Enzymes that do cut:
AcuI AlwNI ApaLI ApoI AvaI BamHI BanI BanII
BbeI BbvCI Bme1580I BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrBI BsrFI BssHII BstAPI BstXI
BstYI Bsu36I BtgI DraIII EaeI EagI EarI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I KasI MscI MspA1I
NaeI NarI NgoMIV PflMI PfoI PmlI PpuMI PstI
SacII SanDI SapI SexAI SfcI SfoI SmaI SrfI
StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI AscI AseI
AsiSI AvrII BaeI BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI BpuEI
BsaBI BsiWI BsmBI BspHI BsrDI BsrGI BssSI BstBI
BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI DrdI
EciI EcoICRI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MslI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SalI SbfI ScaI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI
SwaI Tth111I XbaI XhoI XmnI ZraI