(Linear) (Six-base) MAPSORT of: KIBB0907.seq  Check: 2421  from: 1  to: 282



KIBB0907.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

Acc65I G'GTAC_C

Cuts at:      0     146     282 
   Size:        146     136
 
AflII C'TTAA_G

Cuts at:      0     162     282 
   Size:        162     120
 
BanI G'GyrC_C

Cuts at:      0     146     282 
   Size:        146     136
 
BanII G_rGCy'C

Cuts at:      0     226     282 
   Size:        226      56
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      84     282 
   Size:         84     198
 
Bsp1286I G_dGCh'C

Cuts at:      0     226     282 
   Size:        226      56
 
DraI TTT'AAA

Cuts at:      0      17      86     282 
   Size:         17      69     196

  Fragments arranged by size: 

                 196      69      17
 
HindIII A'AGCT_T

Cuts at:      0     190     282 
   Size:        190      92
 
KpnI G_GTAC'C

Cuts at:      0     150     282 
   Size:        150     132
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     282 
   Size:        115     167
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     130     162     282 
   Size:        130      32     120

  Fragments arranged by size: 

                 130     120      32
 
SmlI C'TyrA_G

Cuts at:      0      99     162     282 
   Size:         99      63     120

  Fragments arranged by size: 

                 120      99      63
 

 Enzymes that do cut:

   Acc65I    AflII     BanI    BanII    BpuEI Bsp1286I     DraI  HindIII
     KpnI     MmeI     PsrI     SmlI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI
     MfeI     MluI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI