(Linear) (Six-base) MAPSORT of: KIBB0911.seq  Check: 9942  from: 1  to: 444



KIBB0911.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AccI GT'mk_AC

Cuts at:      0     314     444 
   Size:        314     130
 
ApaI G_GGCC'C

Cuts at:      0     176     444 
   Size:        176     268
 
AseI AT'TA_AT

Cuts at:      0      95     444 
   Size:         95     349
 
BanI G'GyrC_C

Cuts at:      0      56     444 
   Size:         56     388
 
BanII G_rGCy'C

Cuts at:      0     141     176     444 
   Size:        141      35     268

  Fragments arranged by size: 

                 268     141      35
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     212     444 
   Size:        212     232
 
Bme1580I G_kGCm'C

Cuts at:      0      61     176     444 
   Size:         61     115     268

  Fragments arranged by size: 

                 268     115      61
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     444 
   Size:         83     361
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     116     224     399     408     411     444 
   Size:        116     108     175       9       3      33

  Fragments arranged by size: 

                 175     116     108      33       9       3
 
BsiHKAI G_wGCw'C

Cuts at:      0     141     444 
   Size:        141     303
 
BsmI GAATG_Cn'

Cuts at:      0     402     444 
   Size:        402      42
 
Bsp1286I G_dGCh'C

Cuts at:      0      61     141     176     444 
   Size:         61      80      35     268

  Fragments arranged by size: 

                 268      80      61      35
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     394     444 
   Size:        394      50
 
BtgI C'CryG_G

Cuts at:      0     144     444 
   Size:        144     300
 
BtsI GCAGTG_nn'

Cuts at:      0     311     438     444 
   Size:        311     127       6

  Fragments arranged by size: 

                 311     127       6
 
EarI CTCTTCn'nnn_

Cuts at:      0     417     429     444 
   Size:        417      12      15

  Fragments arranged by size: 

                 417      15      12
 
EcoICRI GAG'CTC

Cuts at:      0     139     444 
   Size:        139     305
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     444 
   Size:         83     361
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     131     444 
   Size:        131     313
 
EcoO109I rG'GnC_Cy

Cuts at:      0     115     173     223     444 
   Size:        115      58      50     221

  Fragments arranged by size: 

                 221     115      58      50
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     276     444 
   Size:        276     168
 
MslI CAynn'nnrTG

Cuts at:      0     392     444 
   Size:        392      52
 
NcoI C'CATG_G

Cuts at:      0     144     444 
   Size:        144     300
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     219     444 
   Size:        219     225
 
PfoI T'CCnGG_A

Cuts at:      0     204     444 
   Size:        204     240
 
PpuMI rG'GwC_Cy

Cuts at:      0     223     444 
   Size:        223     221
 
PspOMI G'GGCC_C

Cuts at:      0     172     444 
   Size:        172     272
 
SacI G_AGCT'C

Cuts at:      0     141     444 
   Size:        141     303
 
SanDI GG'GwC_CC

Cuts at:      0     223     444 
   Size:        223     221
 
SspI AAT'ATT

Cuts at:      0      51     444 
   Size:         51     393
 
StyI C'CwwG_G

Cuts at:      0     144     278     444 
   Size:        144     134     166

  Fragments arranged by size: 

                 166     144     134
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      44      98     444 
   Size:         44      54     346

  Fragments arranged by size: 

                 346      54      44
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     290     322     444 
   Size:        290      32     122

  Fragments arranged by size: 

                 290     122      32
 

 Enzymes that do cut:

     AccI     ApaI     AseI     BanI    BanII    BciVI Bme1580I     BpmI
    BseRI  BsiHKAI     BsmI Bsp1286I    BstXI     BtgI     BtsI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I     MmeI     MslI     NcoI    PflMI
     PfoI    PpuMI   PspOMI     SacI    SanDI     SspI     StyI    TaqII
     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI