Restriction map for
pF1KB0912
(Linear) (Six-base) MAPSORT of: KIBB0912.seq Check: 5341 from: 1 to: 951
KIBB0912.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 419 951
Size: 419 532
AatII G_ACGT'C
Cuts at: 0 466 951
Size: 466 485
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 601 951
Size: 544 57 350
Fragments arranged by size:
544 350 57
AfeI AGC'GCT
Cuts at: 0 691 951
Size: 691 260
AflIII A'CryG_T
Cuts at: 0 154 221 951
Size: 154 67 730
Fragments arranged by size:
730 154 67
AlwNI CAG_nnn'CTG
Cuts at: 0 314 567 774 951
Size: 314 253 207 177
Fragments arranged by size:
314 253 207 177
BanI G'GyrC_C
Cuts at: 0 90 951
Size: 90 861
BbsI GAAGACnn'nnnn_
Cuts at: 0 466 951
Size: 466 485
BbvCI CC'TCA_GC
Cuts at: 0 307 951
Size: 307 644
BglII A'GATC_T
Cuts at: 0 42 951
Size: 42 909
BmrI ACTGGGnnnn_n'
Cuts at: 0 126 193 951
Size: 126 67 758
Fragments arranged by size:
758 126 67
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 475 589 658 951
Size: 385 90 114 69 293
Fragments arranged by size:
385 293 114 90 69
Bpu10I CC'TnA_GC
Cuts at: 0 307 951
Size: 307 644
BsaAI yAC'GTr
Cuts at: 0 825 951
Size: 825 126
BsaHI Gr'CG_yC
Cuts at: 0 463 951
Size: 463 488
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 448 478 951
Size: 448 30 473
Fragments arranged by size:
473 448 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 586 951
Size: 91 495 365
Fragments arranged by size:
495 365 91
BseYI C'CCAG_C
Cuts at: 0 638 951
Size: 638 313
BsiEI CG_ry'CG
Cuts at: 0 185 951
Size: 185 766
BsiHKAI G_wGCw'C
Cuts at: 0 323 674 951
Size: 323 351 277
Fragments arranged by size:
351 323 277
Bsp1286I G_dGCh'C
Cuts at: 0 323 674 951
Size: 323 351 277
Fragments arranged by size:
351 323 277
BspHI T'CATG_A
Cuts at: 0 920 951
Size: 920 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 419 951
Size: 419 532
BssSI C'ACGA_G
Cuts at: 0 751 951
Size: 751 200
BstAPI GCAn_nnn'nTGC
Cuts at: 0 122 951
Size: 122 829
BstYI r'GATC_y
Cuts at: 0 42 593 951
Size: 42 551 358
Fragments arranged by size:
551 358 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 429 951
Size: 429 522
BtsI GCAGTG_nn'
Cuts at: 0 69 549 951
Size: 69 480 402
Fragments arranged by size:
480 402 69
DraIII CAC_nnn'GTG
Cuts at: 0 134 951
Size: 134 817
EaeI y'GGCC_r
Cuts at: 0 776 827 951
Size: 776 51 124
Fragments arranged by size:
776 124 51
EarI CTCTTCn'nnn_
Cuts at: 0 938 951
Size: 938 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 505 951
Size: 505 446
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 475 544 589 601 658 951
Size: 385 90 69 45 12 57 293
Fragments arranged by size:
385 293 90 69 57 45 12
FspI TGC'GCA
Cuts at: 0 932 951
Size: 932 19
HaeII r_GCGC'y
Cuts at: 0 693 951
Size: 693 258
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 448 480 635 642 667 674 696 728
Size: 448 32 155 7 25 7 22 32
Cuts at: 728 951
Size: 223
Fragments arranged by size:
448 223 155 32 32 25 22 7
7
HincII GTy'rAC
Cuts at: 0 36 549 729 951
Size: 36 513 180 222
Fragments arranged by size:
513 222 180 36
MfeI C'AATT_G
Cuts at: 0 578 951
Size: 578 373
MscI TGG'CCA
Cuts at: 0 778 829 951
Size: 778 51 122
Fragments arranged by size:
778 122 51
MspA1I CmG'CkG
Cuts at: 0 469 486 513 638 951
Size: 469 17 27 125 313
Fragments arranged by size:
469 313 125 27 17
NspI r_CATG'y
Cuts at: 0 109 158 225 531 951
Size: 109 49 67 306 420
Fragments arranged by size:
420 306 109 67 49
PasI CC'CwG_GG
Cuts at: 0 768 951
Size: 768 183
PciI A'CATG_T
Cuts at: 0 154 221 951
Size: 154 67 730
Fragments arranged by size:
730 154 67
PflMI CCAn_nnn'nTGG
Cuts at: 0 774 885 951
Size: 774 111 66
Fragments arranged by size:
774 111 66
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 793 825 951
Size: 793 32 126
Fragments arranged by size:
793 126 32
PstI C_TGCA'G
Cuts at: 0 239 951
Size: 239 712
PvuII CAG'CTG
Cuts at: 0 469 486 513 951
Size: 469 17 27 438
Fragments arranged by size:
469 438 27 17
SfcI C'TryA_G
Cuts at: 0 235 267 951
Size: 235 32 684
Fragments arranged by size:
684 235 32
StuI AGG'CCT
Cuts at: 0 914 951
Size: 914 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 145 594 951
Size: 145 449 357
Fragments arranged by size:
449 357 145
TatI w'GTAC_w
Cuts at: 0 84 951
Size: 84 867
ZraI GAC'GTC
Cuts at: 0 464 951
Size: 464 487
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AlwNI BanI BbsI
BbvCI BglII BmrI BpmI Bpu10I BsaAI BsaHI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BssSI
BstAPI BstYI BtgZI BtsI DraIII EaeI EarI EciI
Eco57MI FspI HaeII Hin4I HincII MfeI MscI MspA1I
NspI PasI PciI PflMI PsrI PstI PvuII SfcI
StuI TaqII TatI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AleI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BcgI BciVI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI BpuEI
BsaI BsaBI BsaWI BsgI BsiWI BsmI BsmBI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII BstBI BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraI DrdI EagI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspAI
HindIII HpaI KasI KpnI MluI MmeI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StyI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI