(Linear) (Six-base) MAPSORT of: KIBB0913.seq  Check: 5848  from: 1  to: 309



KIBB0913.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AvaI C'yCGr_G

Cuts at:      0     164     309 
   Size:        164     145
 
BanI G'GyrC_C

Cuts at:      0      43     309 
   Size:         43     266
 
BbeI G_GCGC'C

Cuts at:      0      47     309 
   Size:         47     262
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     174     309 
   Size:        174     135
 
BmgBI CAC'GTC

Cuts at:      0     121     309 
   Size:        121     188
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     309 
   Size:        208     101
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     151     154     309 
   Size:        151       3     155

  Fragments arranged by size: 

                 155     151       3
 
BsaHI Gr'CG_yC

Cuts at:      0      44     128     309 
   Size:         44      84     181

  Fragments arranged by size: 

                 181      84      44
 
BsaWI w'CCGG_w

Cuts at:      0      56     309 
   Size:         56     253
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     172     309 
   Size:        172     137
 
BseYI C'CCAG_C

Cuts at:      0      30     309 
   Size:         30     279
 
BsiHKAI G_wGCw'C

Cuts at:      0     227     309 
   Size:        227      82
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     115     309 
   Size:        115     194
 
Bsp1286I G_dGCh'C

Cuts at:      0     227     309 
   Size:        227      82
 
BsrFI r'CCGG_y

Cuts at:      0      96     309 
   Size:         96     213
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     258     309 
   Size:        258      51
 
BtgI C'CryG_G

Cuts at:      0     251     309 
   Size:        251      58
 
EaeI y'GGCC_r

Cuts at:      0     248     309 
   Size:        248      61
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     309 
   Size:        208     101
 
HaeII r_GCGC'y

Cuts at:      0      47      54     309 
   Size:         47       7     255

  Fragments arranged by size: 

                 255      47       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      93     125     309 
   Size:         93      32     184

  Fragments arranged by size: 

                 184      93      32
 
KasI G'GCGC_C

Cuts at:      0      43     309 
   Size:         43     266
 
MslI CAynn'nnrTG

Cuts at:      0     250     256     309 
   Size:        250       6      53

  Fragments arranged by size: 

                 250      53       6
 
NaeI GCC'GGC

Cuts at:      0      98     309 
   Size:         98     211
 
NarI GG'CG_CC

Cuts at:      0      44     309 
   Size:         44     265
 
NcoI C'CATG_G

Cuts at:      0     251     309 
   Size:        251      58
 
NgoMIV G'CCGG_C

Cuts at:      0      96     309 
   Size:         96     213
 
PfoI T'CCnGG_A

Cuts at:      0     185     309 
   Size:        185     124
 
SfoI GGC'GCC

Cuts at:      0      45     309 
   Size:         45     264
 
SmaI CCC'GGG

Cuts at:      0     166     309 
   Size:        166     143
 
StyI C'CwwG_G

Cuts at:      0      81     251     309 
   Size:         81     170      58

  Fragments arranged by size: 

                 170      81      58
 
XmaI C'CCGG_G

Cuts at:      0     164     309 
   Size:        164     145
 

 Enzymes that do cut:

     AvaI     BanI     BbeI     BbsI    BmgBI     BpmI     BsaI    BsaHI
    BsaWI    BseRI    BseYI  BsiHKAI    BsmBI Bsp1286I    BsrFI    BstXI
     BtgI     EaeI  Eco57MI    HaeII    Hin4I     KasI     MslI     NaeI
     NarI     NcoI   NgoMIV     PfoI     SfoI     SmaI     StyI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BanII    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmnI     ZraI