(Linear) (Six-base) MAPSORT of: KIBB0915.seq  Check: 3338  from: 1  to: 339



KIBB0915.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AgeI A'CCGG_T

Cuts at:      0     100     339 
   Size:        100     239
 
AhdI GACnn_n'nnGTC

Cuts at:      0     101     339 
   Size:        101     238
 
AlwNI CAG_nnn'CTG

Cuts at:      0     264     339 
   Size:        264      75
 
ApaLI G'TGCA_C

Cuts at:      0     177     339 
   Size:        177     162
 
Bme1580I G_kGCm'C

Cuts at:      0     181     188     220     339 
   Size:        181       7      32     119

  Fragments arranged by size: 

                 181     119      32       7
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     109     339 
   Size:        109     230
 
BsaAI yAC'GTr

Cuts at:      0      43     339 
   Size:         43     296
 
BsaWI w'CCGG_w

Cuts at:      0     100     339 
   Size:        100     239
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      27     339 
   Size:         27     312
 
BsiHKAI G_wGCw'C

Cuts at:      0     181     339 
   Size:        181     158
 
Bsp1286I G_dGCh'C

Cuts at:      0     181     188     220     339 
   Size:        181       7      32     119

  Fragments arranged by size: 

                 181     119      32       7
 
BspHI T'CATG_A

Cuts at:      0     107     339 
   Size:        107     232
 
BsrFI r'CCGG_y

Cuts at:      0     100     339 
   Size:        100     239
 
EaeI y'GGCC_r

Cuts at:      0     193     339 
   Size:        193     146
 
EarI CTCTTCn'nnn_

Cuts at:      0     276     339 
   Size:        276      63
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      87     119     339 
   Size:         87      32     220

  Fragments arranged by size: 

                 220      87      32
 
MfeI C'AATT_G

Cuts at:      0      13     339 
   Size:         13     326
 
PfoI T'CCnGG_A

Cuts at:      0      20     339 
   Size:         20     319
 
PmlI CAC'GTG

Cuts at:      0      43     339 
   Size:         43     296
 
StuI AGG'CCT

Cuts at:      0      53     339 
   Size:         53     286
 
StyI C'CwwG_G

Cuts at:      0     196     339 
   Size:        196     143
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      41      78     339 
   Size:         41      37     261

  Fragments arranged by size: 

                 261      41      37
 

 Enzymes that do cut:

     AgeI     AhdI    AlwNI    ApaLI Bme1580I     BsaI    BsaAI    BsaWI
    BseRI  BsiHKAI Bsp1286I    BspHI    BsrFI     EaeI     EarI    Hin4I
     MfeI     PfoI     PmlI     StuI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AleI     AlfI     AloI     ApaI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
    BsaBI    BsaHI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI