Restriction map for
pF1KB0916
(Linear) (Six-base) MAPSORT of: KIBB0916.seq Check: 3903 from: 1 to: 984
KIBB0916.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AccI GT'mk_AC
Cuts at: 0 798 984
Size: 798 186
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 984
Size: 583 401
AflIII A'CryG_T
Cuts at: 0 176 527 916 984
Size: 176 351 389 68
Fragments arranged by size:
389 351 176 68
AleI CACnn'nnGTG
Cuts at: 0 867 984
Size: 867 117
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 885 984
Size: 885 99
AlwNI CAG_nnn'CTG
Cuts at: 0 172 984
Size: 172 812
ApaI G_GGCC'C
Cuts at: 0 308 984
Size: 308 676
ApoI r'AATT_y
Cuts at: 0 211 679 984
Size: 211 468 305
Fragments arranged by size:
468 305 211
AvaI C'yCGr_G
Cuts at: 0 300 357 984
Size: 300 57 627
Fragments arranged by size:
627 300 57
AvrII C'CTAG_G
Cuts at: 0 417 984
Size: 417 567
BanI G'GyrC_C
Cuts at: 0 292 692 734 984
Size: 292 400 42 250
Fragments arranged by size:
400 292 250 42
BanII G_rGCy'C
Cuts at: 0 308 459 893 984
Size: 308 151 434 91
Fragments arranged by size:
434 308 151 91
BbeI G_GCGC'C
Cuts at: 0 296 984
Size: 296 688
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 60 94 984
Size: 60 34 890
Fragments arranged by size:
890 60 34
BlpI GC'TnA_GC
Cuts at: 0 475 514 984
Size: 475 39 470
Fragments arranged by size:
475 470 39
Bme1580I G_kGCm'C
Cuts at: 0 308 697 984
Size: 308 389 287
Fragments arranged by size:
389 308 287
BmgBI CAC'GTC
Cuts at: 0 177 919 984
Size: 177 742 65
Fragments arranged by size:
742 177 65
BmrI ACTGGGnnnn_n'
Cuts at: 0 889 895 984
Size: 889 6 89
Fragments arranged by size:
889 89 6
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 372 984
Size: 372 612
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 724 984
Size: 694 30 260
Fragments arranged by size:
694 260 30
Bpu10I CC'TnA_GC
Cuts at: 0 698 984
Size: 698 286
BsaHI Gr'CG_yC
Cuts at: 0 279 293 984
Size: 279 14 691
Fragments arranged by size:
691 279 14
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 851 875 881 905 984
Size: 851 24 6 24 79
Fragments arranged by size:
851 79 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 936 964 984
Size: 936 28 20
Fragments arranged by size:
936 28 20
BseYI C'CCAG_C
Cuts at: 0 506 710 730 954 984
Size: 506 204 20 224 30
Fragments arranged by size:
506 224 204 30 20
BsiHKAI G_wGCw'C
Cuts at: 0 109 459 864 984
Size: 109 350 405 120
Fragments arranged by size:
405 350 120 109
BsmI GAATG_Cn'
Cuts at: 0 326 984
Size: 326 658
BsmBI CGTCTCn'nnnn_
Cuts at: 0 630 925 984
Size: 630 295 59
Fragments arranged by size:
630 295 59
Bsp1286I G_dGCh'C
Cuts at: 0 109 308 459 697 864 893 984
Size: 109 199 151 238 167 29 91
Fragments arranged by size:
238 199 167 151 109 91 29
BsrDI GCAATG_nn'
Cuts at: 0 311 725 984
Size: 311 414 259
Fragments arranged by size:
414 311 259
BsrFI r'CCGG_y
Cuts at: 0 141 984
Size: 141 843
BstXI CCAn_nnnn'nTGG
Cuts at: 0 730 966 984
Size: 730 236 18
Fragments arranged by size:
730 236 18
BstYI r'GATC_y
Cuts at: 0 158 984
Size: 158 826
BtgI C'CryG_G
Cuts at: 0 820 984
Size: 820 164
BtsI GCAGTG_nn'
Cuts at: 0 51 544 984
Size: 51 493 440
Fragments arranged by size:
493 440 51
EaeI y'GGCC_r
Cuts at: 0 2 139 386 984
Size: 2 137 247 598
Fragments arranged by size:
598 247 137 2
EarI CTCTTCn'nnn_
Cuts at: 0 400 984
Size: 400 584
EcoICRI GAG'CTC
Cuts at: 0 457 984
Size: 457 527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 694 724 984
Size: 583 111 30 260
Fragments arranged by size:
583 260 111 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 854 984
Size: 854 130
EcoO109I rG'GnC_Cy
Cuts at: 0 14 169 196 239 304 305 984
Size: 14 155 27 43 65 1 679
Fragments arranged by size:
679 155 65 43 27 14 1
EcoRI G'AATT_C
Cuts at: 0 211 984
Size: 211 773
EcoRV GAT'ATC
Cuts at: 0 903 984
Size: 903 81
HaeII r_GCGC'y
Cuts at: 0 296 984
Size: 296 688
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 780 812 875 907 984
Size: 780 32 63 32 77
Fragments arranged by size:
780 77 63 32 32
HincII GTy'rAC
Cuts at: 0 87 799 984
Size: 87 712 185
Fragments arranged by size:
712 185 87
KasI G'GCGC_C
Cuts at: 0 292 984
Size: 292 692
MfeI C'AATT_G
Cuts at: 0 382 984
Size: 382 602
MscI TGG'CCA
Cuts at: 0 388 984
Size: 388 596
MslI CAynn'nnrTG
Cuts at: 0 867 984
Size: 867 117
MspA1I CmG'CkG
Cuts at: 0 690 744 984
Size: 690 54 240
Fragments arranged by size:
690 240 54
NaeI GCC'GGC
Cuts at: 0 143 984
Size: 143 841
NarI GG'CG_CC
Cuts at: 0 293 984
Size: 293 691
NgoMIV G'CCGG_C
Cuts at: 0 141 984
Size: 141 843
NspI r_CATG'y
Cuts at: 0 531 984
Size: 531 453
PasI CC'CwG_GG
Cuts at: 0 192 984
Size: 192 792
PciI A'CATG_T
Cuts at: 0 527 984
Size: 527 457
PflMI CCAn_nnn'nTGG
Cuts at: 0 46 530 984
Size: 46 484 454
Fragments arranged by size:
484 454 46
PpuMI rG'GwC_Cy
Cuts at: 0 196 984
Size: 196 788
PspOMI G'GGCC_C
Cuts at: 0 304 984
Size: 304 680
PstI C_TGCA'G
Cuts at: 0 238 749 933 984
Size: 238 511 184 51
Fragments arranged by size:
511 238 184 51
PvuII CAG'CTG
Cuts at: 0 744 984
Size: 744 240
SacI G_AGCT'C
Cuts at: 0 459 984
Size: 459 525
SalI G'TCGA_C
Cuts at: 0 797 984
Size: 797 187
SanDI GG'GwC_CC
Cuts at: 0 196 984
Size: 196 788
SbfI CC_TGCA'GG
Cuts at: 0 238 984
Size: 238 746
SexAI A'CCwGG_T
Cuts at: 0 97 984
Size: 97 887
SfcI C'TryA_G
Cuts at: 0 234 745 929 984
Size: 234 511 184 55
Fragments arranged by size:
511 234 184 55
SfoI GGC'GCC
Cuts at: 0 294 984
Size: 294 690
SmaI CCC'GGG
Cuts at: 0 302 984
Size: 302 682
StyI C'CwwG_G
Cuts at: 0 389 417 984
Size: 389 28 567
Fragments arranged by size:
567 389 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 124 722 984
Size: 124 598 262
Fragments arranged by size:
598 262 124
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 197 229 851 883 984
Size: 197 32 622 32 101
Fragments arranged by size:
622 197 101 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 118 984
Size: 118 866
XmaI C'CCGG_G
Cuts at: 0 300 984
Size: 300 684
XmnI GAAnn'nnTTC
Cuts at: 0 682 984
Size: 682 302
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApaI ApoI
AvaI AvrII BanI BanII BbeI BcgI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaHI BsaXI BseRI
BseYI BsiHKAI BsmI BsmBI Bsp1286I BsrDI BsrFI BstXI
BstYI BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV HaeII Hin4I HincII KasI MfeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PpuMI PspOMI PstI PvuII SacI SalI
SanDI SbfI SexAI SfcI SfoI SmaI StyI TaqII
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaLI AscI AseI AsiSI BaeI BamHI BbsI
BbvCI BciVI BclI BfrBI BglI BglII BmtI BpuEI
BsaI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BspEI
BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI FalI FseI FspI FspAI HindIII
HpaI KpnI MluI MmeI NcoI NdeI NheI NotI
NruI NsiI PacI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SapI ScaI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI XhoI ZraI