(Linear) (Six-base) MAPSORT of: KIBB0917.seq  Check: 6530  from: 1  to: 351



KIBB0917.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     268     351 
   Size:        268      83
 
BanI G'GyrC_C

Cuts at:      0      15     351 
   Size:         15     336
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       9      43     351 
   Size:          9      34     308

  Fragments arranged by size: 

                 308      34       9
 
BfrBI ATG'CAT

Cuts at:      0     112     351 
   Size:        112     239
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     351 
   Size:         28     323
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     216     351 
   Size:        216     135
 
BsaBI GATnn'nnATC

Cuts at:      0     113     351 
   Size:        113     238
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     326     351 
   Size:        326      25
 
BseYI C'CCAG_C

Cuts at:      0     206     351 
   Size:        206     145
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     138     351 
   Size:        138     213
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0       4     351 
   Size:          4     347
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      35     351 
   Size:         35     316
 
BsrDI GCAATG_nn'

Cuts at:      0     179     351 
   Size:        179     172
 
BstEII G'GTnAC_C

Cuts at:      0     130     351 
   Size:        130     221
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     199     351 
   Size:        199     152
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     208     351 
   Size:        208     143
 
EarI CTCTTCn'nnn_

Cuts at:      0     168     351 
   Size:        168     183
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     351 
   Size:         28     323
 
EcoO109I rG'GnC_Cy

Cuts at:      0      24     351 
   Size:         24     327
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     177     209     351 
   Size:        177      32     142

  Fragments arranged by size: 

                 177     142      32
 
MfeI C'AATT_G

Cuts at:      0     102     351 
   Size:        102     249
 
MslI CAynn'nnrTG

Cuts at:      0     184     351 
   Size:        184     167
 
MspA1I CmG'CkG

Cuts at:      0     206     351 
   Size:        206     145
 
NsiI A_TGCA'T

Cuts at:      0     114     351 
   Size:        114     237
 
PpuMI rG'GwC_Cy

Cuts at:      0      24     351 
   Size:         24     327
 
PvuII CAG'CTG

Cuts at:      0     206     351 
   Size:        206     145
 
SapI GCTCTTCn'nnn_

Cuts at:      0     168     351 
   Size:        168     183
 
SfcI C'TryA_G

Cuts at:      0      71     351 
   Size:         71     280
 
StyI C'CwwG_G

Cuts at:      0     282     351 
   Size:        282      69
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     203     351 
   Size:        203     148
 

 Enzymes that do cut:

    AlwNI     BanI     BcgI    BfrBI     BpmI     BsaI    BsaBI    BseRI
    BseYI     BsgI    BsmBI    BspMI    BsrDI   BstEII    BtgZI     DrdI
     EarI  Eco57MI EcoO109I    Hin4I     MfeI     MslI   MspA1I     NsiI
    PpuMI    PvuII     SapI     SfcI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaHI    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI
 Bsp1286I    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI
     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI