(Linear) (Six-base) MAPSORT of: KIBB0921.seq  Check: 3182  from: 1  to: 363



KIBB0921.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

Acc65I G'GTAC_C

Cuts at:      0     278     363 
   Size:        278      85
 
BanI G'GyrC_C

Cuts at:      0     271     278     363 
   Size:        271       7      85

  Fragments arranged by size: 

                 271      85       7
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      53     217     363 
   Size:         53     164     146

  Fragments arranged by size: 

                 164     146      53
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     113     363 
   Size:        113     250
 
BstYI r'GATC_y

Cuts at:      0     308     363 
   Size:        308      55
 
EaeI y'GGCC_r

Cuts at:      0     258     363 
   Size:        258     105
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      53     217     363 
   Size:         53     164     146

  Fragments arranged by size: 

                 164     146      53
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      66      98     363 
   Size:         66      32     265

  Fragments arranged by size: 

                 265      66      32
 
KpnI G_GTAC'C

Cuts at:      0     282     363 
   Size:        282      81
 
SexAI A'CCwGG_T

Cuts at:      0     281     363 
   Size:        281      82
 
SfcI C'TryA_G

Cuts at:      0      64      87     363 
   Size:         64      23     276

  Fragments arranged by size: 

                 276      64      23
 
StuI AGG'CCT

Cuts at:      0      38     363 
   Size:         38     325
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      39     363 
   Size:         39     324
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     134     166     363 
   Size:        134      32     197

  Fragments arranged by size: 

                 197     134      32
 

 Enzymes that do cut:

   Acc65I     BanI     BpmI     BsaI    BstYI     EaeI  Eco57MI    Hin4I
     KpnI    SexAI     SfcI     StuI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StyI     SwaI     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI