Restriction map for
pF1KB0922
(Linear) (Six-base) MAPSORT of: KIBB0922.seq Check: 9968 from: 1 to: 1035
KIBB0922.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
Acc65I G'GTAC_C
Cuts at: 0 940 1035
Size: 940 95
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1011 1035
Size: 1011 24
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 547 970 1035
Size: 547 423 65
Fragments arranged by size:
547 423 65
AlwNI CAG_nnn'CTG
Cuts at: 0 552 808 1035
Size: 552 256 227
Fragments arranged by size:
552 256 227
ApaI G_GGCC'C
Cuts at: 0 240 254 902 1035
Size: 240 14 648 133
Fragments arranged by size:
648 240 133 14
ApaLI G'TGCA_C
Cuts at: 0 145 1035
Size: 145 890
ApoI r'AATT_y
Cuts at: 0 61 1035
Size: 61 974
AvaI C'yCGr_G
Cuts at: 0 156 643 1035
Size: 156 487 392
Fragments arranged by size:
487 392 156
BanI G'GyrC_C
Cuts at: 0 363 940 1035
Size: 363 577 95
Fragments arranged by size:
577 363 95
BanII G_rGCy'C
Cuts at: 0 240 254 347 902 1035
Size: 240 14 93 555 133
Fragments arranged by size:
555 240 133 93 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 679 1035
Size: 679 356
BglI GCCn_nnn'nGGC
Cuts at: 0 503 1035
Size: 503 532
BlpI GC'TnA_GC
Cuts at: 0 818 1035
Size: 818 217
Bme1580I G_kGCm'C
Cuts at: 0 149 240 254 902 1035
Size: 149 91 14 648 133
Fragments arranged by size:
648 149 133 91 14
BmgBI CAC'GTC
Cuts at: 0 168 438 1035
Size: 168 270 597
Fragments arranged by size:
597 270 168
BmrI ACTGGGnnnn_n'
Cuts at: 0 869 1035
Size: 869 166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 574 1035
Size: 279 295 461
Fragments arranged by size:
461 295 279
BsaI GGTCTCn'nnnn_
Cuts at: 0 448 1035
Size: 448 587
BsaAI yAC'GTr
Cuts at: 0 592 1035
Size: 592 443
BsaHI Gr'CG_yC
Cuts at: 0 68 1035
Size: 68 967
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 777 807 1035
Size: 777 30 228
Fragments arranged by size:
777 228 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1025 1035
Size: 1025 10
BseYI C'CCAG_C
Cuts at: 0 908 992 1035
Size: 908 84 43
Fragments arranged by size:
908 84 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 295 460 901 1035
Size: 214 81 165 441 134
Fragments arranged by size:
441 214 165 134 81
BsiEI CG_ry'CG
Cuts at: 0 10 1035
Size: 10 1025
BsiHKAI G_wGCw'C
Cuts at: 0 149 1035
Size: 149 886
BsmI GAATG_Cn'
Cuts at: 0 1004 1035
Size: 1004 31
Bsp1286I G_dGCh'C
Cuts at: 0 149 240 254 347 902 1035
Size: 149 91 14 93 555 133
Fragments arranged by size:
555 149 133 93 91 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 209 923 1035
Size: 209 714 112
Fragments arranged by size:
714 209 112
BsrBI CCG'CTC
Cuts at: 0 110 558 1035
Size: 110 448 477
Fragments arranged by size:
477 448 110
BssHII G'CGCG_C
Cuts at: 0 25 1035
Size: 25 1010
BstXI CCAn_nnnn'nTGG
Cuts at: 0 303 1035
Size: 303 732
BstYI r'GATC_y
Cuts at: 0 152 638 1035
Size: 152 486 397
Fragments arranged by size:
486 397 152
BtsI GCAGTG_nn'
Cuts at: 0 259 849 1035
Size: 259 590 186
Fragments arranged by size:
590 259 186
EaeI y'GGCC_r
Cuts at: 0 7 242 355 379 1035
Size: 7 235 113 24 656
Fragments arranged by size:
656 235 113 24 7
EagI C'GGCC_G
Cuts at: 0 7 1035
Size: 7 1028
EarI CTCTTCn'nnn_
Cuts at: 0 49 1035
Size: 49 986
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 403 1035
Size: 403 632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 574 1011 1035
Size: 279 295 437 24
Fragments arranged by size:
437 295 279 24
EcoO109I rG'GnC_Cy
Cuts at: 0 237 251 549 790 805 898 899 912
Size: 237 14 298 241 15 93 1 13
Cuts at: 912 1035
Size: 123
Fragments arranged by size:
298 241 237 123 93 15 14 13
1
FspI TGC'GCA
Cuts at: 0 373 1035
Size: 373 662
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 777 809 1035
Size: 777 32 226
Fragments arranged by size:
777 226 32
KpnI G_GTAC'C
Cuts at: 0 944 1035
Size: 944 91
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 341 1035
Size: 102 239 694
Fragments arranged by size:
694 239 102
MscI TGG'CCA
Cuts at: 0 244 1035
Size: 244 791
MslI CAynn'nnrTG
Cuts at: 0 436 482 529 1035
Size: 436 46 47 506
Fragments arranged by size:
506 436 47 46
MspA1I CmG'CkG
Cuts at: 0 248 299 330 1035
Size: 248 51 31 705
Fragments arranged by size:
705 248 51 31
PasI CC'CwG_GG
Cuts at: 0 501 546 1035
Size: 501 45 489
Fragments arranged by size:
501 489 45
PflMI CCAn_nnn'nTGG
Cuts at: 0 552 1035
Size: 552 483
PfoI T'CCnGG_A
Cuts at: 0 116 564 1035
Size: 116 448 471
Fragments arranged by size:
471 448 116
PpuMI rG'GwC_Cy
Cuts at: 0 790 912 1035
Size: 790 122 123
Fragments arranged by size:
790 123 122
PspOMI G'GGCC_C
Cuts at: 0 236 250 898 1035
Size: 236 14 648 137
Fragments arranged by size:
648 236 137 14
PspXI vC'TCGA_Gb
Cuts at: 0 643 1035
Size: 643 392
PstI C_TGCA'G
Cuts at: 0 329 804 1035
Size: 329 475 231
Fragments arranged by size:
475 329 231
SapI GCTCTTCn'nnn_
Cuts at: 0 49 1035
Size: 49 986
SexAI A'CCwGG_T
Cuts at: 0 848 1035
Size: 848 187
SfcI C'TryA_G
Cuts at: 0 325 800 1035
Size: 325 475 235
Fragments arranged by size:
475 325 235
SmaI CCC'GGG
Cuts at: 0 158 1035
Size: 158 877
SmlI C'TyrA_G
Cuts at: 0 643 1035
Size: 643 392
StyI C'CwwG_G
Cuts at: 0 677 1035
Size: 677 358
TatI w'GTAC_w
Cuts at: 0 610 1035
Size: 610 425
Tth111I GACn'n_nGTC
Cuts at: 0 850 1035
Size: 850 185
XhoI C'TCGA_G
Cuts at: 0 643 1035
Size: 643 392
XmaI C'CCGG_G
Cuts at: 0 156 1035
Size: 156 879
Enzymes that do cut:
Acc65I AcuI AlfI AlwNI ApaI ApaLI ApoI AvaI
BanI BanII BbsI BglI BlpI Bme1580I BmgBI BmrI
BpmI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BssHII BstXI
BstYI BtsI EaeI EagI EarI EciI Eco57MI EcoO109I
FspI Hin4I KpnI MmeI MscI MslI MspA1I PasI
PflMI PfoI PpuMI PspOMI PspXI PstI SapI SexAI
SfcI SmaI SmlI StyI TatI Tth111I XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BmtI BplI Bpu10I BpuEI BsaBI BsaWI BsiWI BsmBI
BspEI BspHI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspAI HaeII HincII HindIII HpaI KasI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PshAI
PsiI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TstI
XbaI XcmI XmnI ZraI