Restriction map for
pF1KB0930
(Linear) (Six-base) MAPSORT of: KIBB0930.seq Check: 130 from: 1 to: 1113
KIBB0930.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 105 354 748 975 1113
Size: 12 93 249 394 227 138
Fragments arranged by size:
394 249 227 138 93 12
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 844 876 1113
Size: 844 32 237
Fragments arranged by size:
844 237 32
ApaI G_GGCC'C
Cuts at: 0 93 1113
Size: 93 1020
ApaLI G'TGCA_C
Cuts at: 0 589 841 1113
Size: 589 252 272
Fragments arranged by size:
589 272 252
AvaI C'yCGr_G
Cuts at: 0 619 1113
Size: 619 494
BanII G_rGCy'C
Cuts at: 0 93 309 1113
Size: 93 216 804
Fragments arranged by size:
804 216 93
BbsI GAAGACnn'nnnn_
Cuts at: 0 212 1113
Size: 212 901
BbvCI CC'TCA_GC
Cuts at: 0 907 1113
Size: 907 206
BclI T'GATC_A
Cuts at: 0 291 1113
Size: 291 822
BlpI GC'TnA_GC
Cuts at: 0 553 743 1113
Size: 553 190 370
Fragments arranged by size:
553 370 190
Bme1580I G_kGCm'C
Cuts at: 0 93 593 619 845 1113
Size: 93 500 26 226 268
Fragments arranged by size:
500 268 226 93 26
BmgBI CAC'GTC
Cuts at: 0 36 105 1113
Size: 36 69 1008
Fragments arranged by size:
1008 69 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 1113
Size: 395 718
Bpu10I CC'TnA_GC
Cuts at: 0 907 1113
Size: 907 206
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 1113
Size: 36 1077
BsaI GGTCTCn'nnnn_
Cuts at: 0 210 1113
Size: 210 903
BsaAI yAC'GTr
Cuts at: 0 324 539 1113
Size: 324 215 574
Fragments arranged by size:
574 324 215
BsaHI Gr'CG_yC
Cuts at: 0 425 1113
Size: 425 688
BsaWI w'CCGG_w
Cuts at: 0 638 890 974 1001 1113
Size: 638 252 84 27 112
Fragments arranged by size:
638 252 112 84 27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 52 82 181 211 844 874 1113
Size: 52 30 99 30 633 30 239
Fragments arranged by size:
633 239 99 52 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 205 1113
Size: 73 132 908
Fragments arranged by size:
908 132 73
BseYI C'CCAG_C
Cuts at: 0 79 1113
Size: 79 1034
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 994 1113
Size: 256 738 119
Fragments arranged by size:
738 256 119
BsiEI CG_ry'CG
Cuts at: 0 481 859 1113
Size: 481 378 254
Fragments arranged by size:
481 378 254
BsiHKAI G_wGCw'C
Cuts at: 0 458 593 770 845 1113
Size: 458 135 177 75 268
Fragments arranged by size:
458 268 177 135 75
BsmBI CGTCTCn'nnnn_
Cuts at: 0 174 1113
Size: 174 939
Bsp1286I G_dGCh'C
Cuts at: 0 93 309 458 593 619 770 845 1113
Size: 93 216 149 135 26 151 75 268
Fragments arranged by size:
268 216 151 149 135 93 75 26
BspEI T'CCGG_A
Cuts at: 0 890 1113
Size: 890 223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 662 1113
Size: 662 451
BsrBI CCG'CTC
Cuts at: 0 855 1113
Size: 855 258
BsrFI r'CCGG_y
Cuts at: 0 566 1113
Size: 566 547
BssHII G'CGCG_C
Cuts at: 0 507 1113
Size: 507 606
BssSI C'ACGA_G
Cuts at: 0 780 1113
Size: 780 333
BtgI C'CryG_G
Cuts at: 0 196 434 1113
Size: 196 238 679
Fragments arranged by size:
679 238 196
BtsI GCAGTG_nn'
Cuts at: 0 132 239 1113
Size: 132 107 874
Fragments arranged by size:
874 132 107
EaeI y'GGCC_r
Cuts at: 0 38 250 478 627 856 1038 1113
Size: 38 212 228 149 229 182 75
Fragments arranged by size:
229 228 212 182 149 75 38
EagI C'GGCC_G
Cuts at: 0 478 856 1113
Size: 478 378 257
Fragments arranged by size:
478 378 257
EarI CTCTTCn'nnn_
Cuts at: 0 1015 1113
Size: 1015 98
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 105 354 395 748 975 1113
Size: 12 93 249 41 353 227 138
Fragments arranged by size:
353 249 227 138 93 41 12
EcoO109I rG'GnC_Cy
Cuts at: 0 89 90 244 773 1113
Size: 89 1 154 529 340
Fragments arranged by size:
529 340 154 89 1
HaeII r_GCGC'y
Cuts at: 0 498 553 663 1113
Size: 498 55 110 450
Fragments arranged by size:
498 450 110 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 149 154 181 186 1113
Size: 149 5 27 5 927
Fragments arranged by size:
927 149 27 5 5
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 842 1113
Size: 842 271
MscI TGG'CCA
Cuts at: 0 40 1113
Size: 40 1073
MspA1I CmG'CkG
Cuts at: 0 557 837 932 1113
Size: 557 280 95 181
Fragments arranged by size:
557 280 181 95
NaeI GCC'GGC
Cuts at: 0 568 1113
Size: 568 545
NgoMIV G'CCGG_C
Cuts at: 0 566 1113
Size: 566 547
PmlI CAC'GTG
Cuts at: 0 324 539 1113
Size: 324 215 574
Fragments arranged by size:
574 324 215
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 508 540 844 876 1113
Size: 508 32 304 32 237
Fragments arranged by size:
508 304 237 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 244 1113
Size: 244 869
PspOMI G'GGCC_C
Cuts at: 0 89 1113
Size: 89 1024
PstI C_TGCA'G
Cuts at: 0 273 659 1113
Size: 273 386 454
Fragments arranged by size:
454 386 273
PvuII CAG'CTG
Cuts at: 0 557 837 1113
Size: 557 280 276
Fragments arranged by size:
557 280 276
SanDI GG'GwC_CC
Cuts at: 0 244 1113
Size: 244 869
SfcI C'TryA_G
Cuts at: 0 269 655 1113
Size: 269 386 458
Fragments arranged by size:
458 386 269
SmlI C'TyrA_G
Cuts at: 0 51 1113
Size: 51 1062
StuI AGG'CCT
Cuts at: 0 607 706 1113
Size: 607 99 407
Fragments arranged by size:
607 407 99
StyI C'CwwG_G
Cuts at: 0 602 896 983 1113
Size: 602 294 87 130
Fragments arranged by size:
602 294 130 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 52 84 181 213 1113
Size: 52 32 97 32 900
Fragments arranged by size:
900 97 52 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 166 1113
Size: 166 947
XmnI GAAnn'nnTTC
Cuts at: 0 367 691 802 1024 1054 1113
Size: 367 324 111 222 30 59
Fragments arranged by size:
367 324 222 111 59 30
Enzymes that do cut:
AcuI AloI ApaI ApaLI AvaI BanII BbsI BbvCI
BclI BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII
BssSI BtgI BtsI EaeI EagI EarI Eco57MI EcoO109I
HaeII Hin4I MmeI MscI MspA1I NaeI NgoMIV PmlI
PpiI PpuMI PspOMI PstI PvuII SanDI SfcI SmlI
StuI StyI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BfrBI BglI BglII BmrI BmtI BplI BsaBI BsiWI
BsmI BspHI BsrDI BsrGI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MslI NarI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TatI XbaI XcmI XhoI XmaI ZraI