Restriction map for
pF1KB0939
(Linear) (Six-base) MAPSORT of: KIBB0939.seq Check: 125 from: 1 to: 480
KIBB0939.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 480
Size: 230 250
ApaI G_GGCC'C
Cuts at: 0 46 480
Size: 46 434
AvaI C'yCGr_G
Cuts at: 0 301 480
Size: 301 179
BanII G_rGCy'C
Cuts at: 0 46 404 480
Size: 46 358 76
Fragments arranged by size:
358 76 46
Bme1580I G_kGCm'C
Cuts at: 0 46 480
Size: 46 434
BmrI ACTGGGnnnn_n'
Cuts at: 0 210 480
Size: 210 270
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 250 371 427 480
Size: 110 140 121 56 53
Fragments arranged by size:
140 121 110 56 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 480
Size: 255 225
BsaI GGTCTCn'nnnn_
Cuts at: 0 6 480
Size: 6 474
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 439 469 480
Size: 439 30 11
Fragments arranged by size:
439 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 98 110 113 247 460 480
Size: 98 12 3 134 213 20
Fragments arranged by size:
213 134 98 20 12 3
BseYI C'CCAG_C
Cuts at: 0 362 480
Size: 362 118
BsiHKAI G_wGCw'C
Cuts at: 0 404 480
Size: 404 76
Bsp1286I G_dGCh'C
Cuts at: 0 46 404 480
Size: 46 358 76
Fragments arranged by size:
358 76 46
BsrBI CCG'CTC
Cuts at: 0 158 480
Size: 158 322
Bsu36I CC'TnA_GG
Cuts at: 0 50 480
Size: 50 430
EarI CTCTTCn'nnn_
Cuts at: 0 20 480
Size: 20 460
EcoICRI GAG'CTC
Cuts at: 0 402 480
Size: 402 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 230 250 371 427 480
Size: 110 120 20 121 56 53
Fragments arranged by size:
121 120 110 56 53 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 125 480
Size: 125 355
EcoO109I rG'GnC_Cy
Cuts at: 0 42 43 131 151 455 480
Size: 42 1 88 20 304 25
Fragments arranged by size:
304 88 42 25 20 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 439 471 480
Size: 439 32 9
Fragments arranged by size:
439 32 9
MspA1I CmG'CkG
Cuts at: 0 351 366 480
Size: 351 15 114
Fragments arranged by size:
351 114 15
PasI CC'CwG_GG
Cuts at: 0 147 480
Size: 147 333
PflMI CCAn_nnn'nTGG
Cuts at: 0 354 480
Size: 354 126
PfoI T'CCnGG_A
Cuts at: 0 404 480
Size: 404 76
PpuMI rG'GwC_Cy
Cuts at: 0 131 151 455 480
Size: 131 20 304 25
Fragments arranged by size:
304 131 25 20
PspOMI G'GGCC_C
Cuts at: 0 42 480
Size: 42 438
PstI C_TGCA'G
Cuts at: 0 182 480
Size: 182 298
SacI G_AGCT'C
Cuts at: 0 404 480
Size: 404 76
SanDI GG'GwC_CC
Cuts at: 0 131 151 480
Size: 131 20 329
Fragments arranged by size:
329 131 20
SexAI A'CCwGG_T
Cuts at: 0 200 480
Size: 200 280
SfcI C'TryA_G
Cuts at: 0 178 480
Size: 178 302
SmlI C'TyrA_G
Cuts at: 0 270 301 480
Size: 270 31 179
Fragments arranged by size:
270 179 31
StyI C'CwwG_G
Cuts at: 0 94 480
Size: 94 386
XhoI C'TCGA_G
Cuts at: 0 301 480
Size: 301 179
XmnI GAAnn'nnTTC
Cuts at: 0 191 480
Size: 191 289
Enzymes that do cut:
AcuI ApaI AvaI BanII Bme1580I BmrI BpmI BpuEI
BsaI BsaXI BseRI BseYI BsiHKAI Bsp1286I BsrBI Bsu36I
EarI EcoICRI Eco57MI EcoNI EcoO109I Hin4I MspA1I PasI
PflMI PfoI PpuMI PspOMI PstI SacI SanDI SexAI
SfcI SmlI StyI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaHI
BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI