Restriction map for
pF1KB0940
(Linear) (Six-base) MAPSORT of: KIBB0940.seq Check: 6621 from: 1 to: 1170
KIBB0940.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AatII G_ACGT'C
Cuts at: 0 332 1170
Size: 332 838
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 923 1170
Size: 923 247
AflIII A'CryG_T
Cuts at: 0 707 1170
Size: 707 463
AgeI A'CCGG_T
Cuts at: 0 512 1170
Size: 512 658
AseI AT'TA_AT
Cuts at: 0 971 1170
Size: 971 199
BanII G_rGCy'C
Cuts at: 0 193 1170
Size: 193 977
BmgBI CAC'GTC
Cuts at: 0 710 1170
Size: 710 460
BsaBI GATnn'nnATC
Cuts at: 0 582 1170
Size: 582 588
BsaHI Gr'CG_yC
Cuts at: 0 329 1170
Size: 329 841
BsaWI w'CCGG_w
Cuts at: 0 512 1170
Size: 512 658
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1058 1088 1141 1170
Size: 1058 30 53 29
Fragments arranged by size:
1058 53 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 119 183 1170
Size: 119 64 987
Fragments arranged by size:
987 119 64
BseYI C'CCAG_C
Cuts at: 0 181 772 1170
Size: 181 591 398
Fragments arranged by size:
591 398 181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 1012 1170
Size: 118 894 158
Fragments arranged by size:
894 158 118
BsiHKAI G_wGCw'C
Cuts at: 0 1110 1170
Size: 1110 60
Bsp1286I G_dGCh'C
Cuts at: 0 193 1110 1170
Size: 193 917 60
Fragments arranged by size:
917 193 60
BspHI T'CATG_A
Cuts at: 0 648 857 1170
Size: 648 209 313
Fragments arranged by size:
648 313 209
BsrFI r'CCGG_y
Cuts at: 0 102 512 1170
Size: 102 410 658
Fragments arranged by size:
658 410 102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 174 1170
Size: 174 996
BtgI C'CryG_G
Cuts at: 0 120 173 1061 1170
Size: 120 53 888 109
Fragments arranged by size:
888 120 109 53
BtsI GCAGTG_nn'
Cuts at: 0 738 1162 1170
Size: 738 424 8
Fragments arranged by size:
738 424 8
ClaI AT'CG_AT
Cuts at: 0 548 1170
Size: 548 622
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 807 842 1170
Size: 807 35 328
Fragments arranged by size:
807 328 35
DraI TTT'AAA
Cuts at: 0 212 1170
Size: 212 958
DraIII CAC_nnn'GTG
Cuts at: 0 718 1170
Size: 718 452
EaeI y'GGCC_r
Cuts at: 0 702 1170
Size: 702 468
EarI CTCTTCn'nnn_
Cuts at: 0 253 1170
Size: 253 917
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 923 1170
Size: 923 247
EcoO109I rG'GnC_Cy
Cuts at: 0 75 688 1114 1170
Size: 75 613 426 56
Fragments arranged by size:
613 426 75 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 38 70 230 262 319 351 616 646
Size: 38 32 160 32 57 32 265 30
Cuts at: 646 648 678 929 961 1170
Size: 2 30 251 32 209
Fragments arranged by size:
265 251 209 160 57 38 32 32
32 32 30 30 2
MfeI C'AATT_G
Cuts at: 0 960 1170
Size: 960 210
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1037 1170
Size: 1037 133
MscI TGG'CCA
Cuts at: 0 704 1170
Size: 704 466
MslI CAynn'nnrTG
Cuts at: 0 1138 1170
Size: 1138 32
MspA1I CmG'CkG
Cuts at: 0 698 1170
Size: 698 472
NcoI C'CATG_G
Cuts at: 0 173 1061 1170
Size: 173 888 109
Fragments arranged by size:
888 173 109
NdeI CA'TA_TG
Cuts at: 0 1094 1170
Size: 1094 76
NspI r_CATG'y
Cuts at: 0 85 1170
Size: 85 1085
PpuMI rG'GwC_Cy
Cuts at: 0 75 1114 1170
Size: 75 1039 56
Fragments arranged by size:
1039 75 56
PsiI TTA'TAA
Cuts at: 0 1043 1170
Size: 1043 127
SanDI GG'GwC_CC
Cuts at: 0 75 1170
Size: 75 1095
SphI G_CATG'C
Cuts at: 0 85 1170
Size: 85 1085
StyI C'CwwG_G
Cuts at: 0 173 1061 1170
Size: 173 888 109
Fragments arranged by size:
888 173 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1141 1170
Size: 1141 29
ZraI GAC'GTC
Cuts at: 0 330 1170
Size: 330 840
Enzymes that do cut:
AatII AcuI AflIII AgeI AseI BanII BmgBI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BsrFI BstXI BtgI BtsI ClaI CspCI DraI
DraIII EaeI EarI Eco57MI EcoO109I Hin4I MfeI MmeI
MscI MslI MspA1I NcoI NdeI NspI PpuMI PsiI
SanDI SphI StyI TstI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgZI DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MluI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StuI SwaI TaqII
TatI Tth111I XbaI XcmI XhoI XmaI XmnI