(Linear) (Six-base) MAPSORT of: KIBB0942.seq  Check: 8133  from: 1  to: 1176



KIBB0942.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     367    1176 
   Size:        367     809
 
AflII C'TTAA_G

Cuts at:      0     826    1176 
   Size:        826     350
 
AflIII A'CryG_T

Cuts at:      0     749     889    1176 
   Size:        749     140     287

  Fragments arranged by size: 

                 749     287     140
 
AleI CACnn'nnGTG

Cuts at:      0     705    1176 
   Size:        705     471
 
AlwNI CAG_nnn'CTG

Cuts at:      0     145     646     661    1176 
   Size:        145     501      15     515

  Fragments arranged by size: 

                 515     501     145      15
 
ApaLI G'TGCA_C

Cuts at:      0    1012    1176 
   Size:       1012     164
 
ApoI r'AATT_y

Cuts at:      0     265     617     631     718     764    1176 
   Size:        265     352      14      87      46     412

  Fragments arranged by size: 

                 412     352     265      87      46      14
 
AvaI C'yCGr_G

Cuts at:      0      61    1176 
   Size:         61    1115
 
BanI G'GyrC_C

Cuts at:      0     595    1176 
   Size:        595     581
 
BanII G_rGCy'C

Cuts at:      0      62     231    1176 
   Size:         62     169     945

  Fragments arranged by size: 

                 945     169      62
 
Bme1580I G_kGCm'C

Cuts at:      0    1016    1176 
   Size:       1016     160
 
BmgBI CAC'GTC

Cuts at:      0     890    1176 
   Size:        890     286
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     179    1176 
   Size:        179     997
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     683     926    1176 
   Size:        683     243     250

  Fragments arranged by size: 

                 683     250     243
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     112     142    1176 
   Size:        112      30    1034

  Fragments arranged by size: 

                1034     112      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     539    1176 
   Size:        539     637
 
BsiHKAI G_wGCw'C

Cuts at:      0    1016    1176 
   Size:       1016     160
 
BsmI GAATG_Cn'

Cuts at:      0     346     472    1176 
   Size:        346     126     704

  Fragments arranged by size: 

                 704     346     126
 
Bsp1286I G_dGCh'C

Cuts at:      0      62     231    1016    1176 
   Size:         62     169     785     160

  Fragments arranged by size: 

                 785     169     160      62
 
BspHI T'CATG_A

Cuts at:      0     729    1176 
   Size:        729     447
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      18    1176 
   Size:         18    1158
 
BsrBI CCG'CTC

Cuts at:      0     298    1176 
   Size:        298     878
 
BsrDI GCAATG_nn'

Cuts at:      0      91    1176 
   Size:         91    1085
 
BsrGI T'GTAC_A

Cuts at:      0      48    1176 
   Size:         48    1128
 
BssSI C'ACGA_G

Cuts at:      0      79    1176 
   Size:         79    1097
 
BstEII G'GTnAC_C

Cuts at:      0     375    1176 
   Size:        375     801
 
BtsI GCAGTG_nn'

Cuts at:      0     122     578     662    1176 
   Size:        122     456      84     514

  Fragments arranged by size: 

                 514     456     122      84
 
DraI TTT'AAA

Cuts at:      0     723    1176 
   Size:        723     453
 
EarI CTCTTCn'nnn_

Cuts at:      0    1108    1138    1176 
   Size:       1108      30      38

  Fragments arranged by size: 

                1108      38      30
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     367     683     926    1176 
   Size:        367     316     243     250

  Fragments arranged by size: 

                 367     316     250     243
 
EcoO109I rG'GnC_Cy

Cuts at:      0      30    1176 
   Size:         30    1146
 
EcoRV GAT'ATC

Cuts at:      0    1103    1176 
   Size:       1103      73
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     952    1176 
   Size:        952     224
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     568     600    1176 
   Size:        568      32     576

  Fragments arranged by size: 

                 576     568      32
 
HindIII A'AGCT_T

Cuts at:      0     823    1176 
   Size:        823     353
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1115    1176 
   Size:       1115      61
 
MslI CAynn'nnrTG

Cuts at:      0     197     242     705     814    1176 
   Size:        197      45     463     109     362

  Fragments arranged by size: 

                 463     362     197     109      45
 
MspA1I CmG'CkG

Cuts at:      0     946    1176 
   Size:        946     230
 
NspI r_CATG'y

Cuts at:      0     753    1176 
   Size:        753     423
 
PciI A'CATG_T

Cuts at:      0     749    1176 
   Size:        749     427
 
PpuMI rG'GwC_Cy

Cuts at:      0      30    1176 
   Size:         30    1146
 
PsiI TTA'TAA

Cuts at:      0     679    1176 
   Size:        679     497
 
PstI C_TGCA'G

Cuts at:      0     951    1176 
   Size:        951     225
 
PvuII CAG'CTG

Cuts at:      0     946    1176 
   Size:        946     230
 
SfcI C'TryA_G

Cuts at:      0     947    1176 
   Size:        947     229
 
SmlI C'TyrA_G

Cuts at:      0     826    1176 
   Size:        826     350
 
SpeI A'CTAG_T

Cuts at:      0     702    1176 
   Size:        702     474
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     410    1176 
   Size:        410     766
 
TatI w'GTAC_w

Cuts at:      0      48    1176 
   Size:         48    1128
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     112     144    1176 
   Size:        112      32    1032

  Fragments arranged by size: 

                1032     112      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     188     935    1176 
   Size:        188     747     241

  Fragments arranged by size: 

                 747     241     188
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AleI    AlwNI    ApaLI     ApoI     AvaI
     BanI    BanII Bme1580I    BmgBI     BmrI     BpmI    BsaXI    BseRI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrGI
    BssSI   BstEII     BtsI     DraI     EarI  Eco57MI EcoO109I    EcoRV
     FalI    Hin4I  HindIII     MmeI     MslI   MspA1I     NspI     PciI
    PpuMI     PsiI     PstI    PvuII     SfcI     SmlI     SpeI    TaqII
     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AlfI     AloI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BsrFI   BssHII   BstAPI    BstBI    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     SspI
     StuI     StyI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI