(Linear) (Six-base) MAPSORT of: KIBB0945.seq Check: 1331 from: 1 to: 510
KIBB0945.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 0 209 398 510
Size: 209 189 112
Fragments arranged by size:
209 189 112
ApoI r'AATT_y
Cuts at: 0 220 454 510
Size: 220 234 56
Fragments arranged by size:
234 220 56
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 252 510
Size: 252 258
BsaHI Gr'CG_yC
Cuts at: 0 233 510
Size: 233 277
BsiHKAI G_wGCw'C
Cuts at: 0 37 510
Size: 37 473
BsmBI CGTCTCn'nnnn_
Cuts at: 0 240 510
Size: 240 270
Bsp1286I G_dGCh'C
Cuts at: 0 37 510
Size: 37 473
DraI TTT'AAA
Cuts at: 0 216 510
Size: 216 294
EarI CTCTTCn'nnn_
Cuts at: 0 116 340 510
Size: 116 224 170
Fragments arranged by size:
224 170 116
EcoRI G'AATT_C
Cuts at: 0 220 454 510
Size: 220 234 56
Fragments arranged by size:
234 220 56
EcoRV GAT'ATC
Cuts at: 0 483 510
Size: 483 27
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 38 510
Size: 38 472
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 115 147 510
Size: 115 32 363
Fragments arranged by size:
363 115 32
HindIII A'AGCT_T
Cuts at: 0 85 510
Size: 85 425
MslI CAynn'nnrTG
Cuts at: 0 44 510
Size: 44 466
TatI w'GTAC_w
Cuts at: 0 162 510
Size: 162 348
Enzymes that do cut:
AccI ApoI BplI BsaHI BsiHKAI BsmBI Bsp1286I DraI
EarI EcoRI EcoRV FalI Hin4I HindIII MslI TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiWI BsmI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI
Eco57MI EcoNI EcoO109I FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI