Restriction map for
pF1KB0946
(Linear) (Six-base) MAPSORT of: KIBB0946.seq Check: 4337 from: 1 to: 1197
KIBB0946.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AccI GT'mk_AC
Cuts at: 0 394 1197
Size: 394 803
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 1196 1197
Size: 446 750 1
Fragments arranged by size:
750 446 1
ApoI r'AATT_y
Cuts at: 0 322 330 342 432 462 522 662 914
Size: 322 8 12 90 30 60 140 252
Cuts at: 914 1082 1197
Size: 168 115
Fragments arranged by size:
322 252 168 140 115 90 60 30
12 8
BanII G_rGCy'C
Cuts at: 0 185 1197
Size: 185 1012
BbsI GAAGACnn'nnnn_
Cuts at: 0 712 1197
Size: 712 485
BclI T'GATC_A
Cuts at: 0 155 1197
Size: 155 1042
BfrBI ATG'CAT
Cuts at: 0 776 1197
Size: 776 421
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 578 1197
Size: 578 619
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 678 1028 1197
Size: 678 350 169
Fragments arranged by size:
678 350 169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1108 1197
Size: 1108 89
BsaI GGTCTCn'nnnn_
Cuts at: 0 126 1197
Size: 126 1071
BsiHKAI G_wGCw'C
Cuts at: 0 832 1197
Size: 832 365
BsmI GAATG_Cn'
Cuts at: 0 364 1114 1197
Size: 364 750 83
Fragments arranged by size:
750 364 83
BsmBI CGTCTCn'nnnn_
Cuts at: 0 983 1197
Size: 983 214
Bsp1286I G_dGCh'C
Cuts at: 0 185 832 1197
Size: 185 647 365
Fragments arranged by size:
647 365 185
BspHI T'CATG_A
Cuts at: 0 42 1197
Size: 42 1155
BtgI C'CryG_G
Cuts at: 0 717 801 1197
Size: 717 84 396
Fragments arranged by size:
717 396 84
BtsI GCAGTG_nn'
Cuts at: 0 615 1197
Size: 615 582
DraI TTT'AAA
Cuts at: 0 521 1197
Size: 521 676
EarI CTCTTCn'nnn_
Cuts at: 0 41 1197
Size: 41 1156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 678 1028 1196 1197
Size: 446 232 350 168 1
Fragments arranged by size:
446 350 232 168 1
EcoRI G'AATT_C
Cuts at: 0 432 662 914 1082 1197
Size: 432 230 252 168 115
Fragments arranged by size:
432 252 230 168 115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 205 237 581 613 1197
Size: 205 32 344 32 584
Fragments arranged by size:
584 344 205 32 32
HindIII A'AGCT_T
Cuts at: 0 1043 1197
Size: 1043 154
MslI CAynn'nnrTG
Cuts at: 0 443 1197
Size: 443 754
NcoI C'CATG_G
Cuts at: 0 717 801 1197
Size: 717 84 396
Fragments arranged by size:
717 396 84
NdeI CA'TA_TG
Cuts at: 0 150 1197
Size: 150 1047
NsiI A_TGCA'T
Cuts at: 0 778 1197
Size: 778 419
NspI r_CATG'y
Cuts at: 0 59 608 1197
Size: 59 549 589
Fragments arranged by size:
589 549 59
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 1197
Size: 106 32 1059
Fragments arranged by size:
1059 106 32
PsiI TTA'TAA
Cuts at: 0 484 1197
Size: 484 713
SexAI A'CCwGG_T
Cuts at: 0 116 1197
Size: 116 1081
SfcI C'TryA_G
Cuts at: 0 29 125 1053 1197
Size: 29 96 928 144
Fragments arranged by size:
928 144 96 29
SmlI C'TyrA_G
Cuts at: 0 1123 1197
Size: 1123 74
SphI G_CATG'C
Cuts at: 0 59 1197
Size: 59 1138
StuI AGG'CCT
Cuts at: 0 793 961 1197
Size: 793 168 236
Fragments arranged by size:
793 236 168
StyI C'CwwG_G
Cuts at: 0 717 801 1197
Size: 717 84 396
Fragments arranged by size:
717 396 84
XmnI GAAnn'nnTTC
Cuts at: 0 1045 1197
Size: 1045 152
Enzymes that do cut:
AccI AcuI ApoI BanII BbsI BclI BfrBI BplI
BpmI BpuEI BsaI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BtgI BtsI DraI EarI Eco57MI EcoRI Hin4I HindIII
MslI NcoI NdeI NsiI NspI PpiI PsiI SexAI
SfcI SmlI SphI StuI StyI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BciVI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiWI BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII
DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI
NgoMIV NheI NotI NruI PacI PasI PciI PflMI
PfoI PmeI PmlI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI