(Linear) (Six-base) MAPSORT of: KIBB0947.seq  Check: 7731  from: 1  to: 534



KIBB0947.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AccI GT'mk_AC

Cuts at:      0     286     534 
   Size:        286     248
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     534 
   Size:        256     278
 
AlwNI CAG_nnn'CTG

Cuts at:      0     526     534 
   Size:        526       8
 
BamHI G'GATC_C

Cuts at:      0      27     534 
   Size:         27     507
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     150     534 
   Size:        150     384
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338     534 
   Size:        338     196
 
Bpu10I CC'TnA_GC

Cuts at:      0     406     534 
   Size:        406     128
 
BseYI C'CCAG_C

Cuts at:      0     291     466     534 
   Size:        291     175      68

  Fragments arranged by size: 

                 291     175      68
 
BspHI T'CATG_A

Cuts at:      0     503     534 
   Size:        503      31
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      99     366     534 
   Size:         99     267     168

  Fragments arranged by size: 

                 267     168      99
 
BsrFI r'CCGG_y

Cuts at:      0       3     534 
   Size:          3     531
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     349     534 
   Size:        349     185
 
BstYI r'GATC_y

Cuts at:      0      27     534 
   Size:         27     507
 
BstZ17I GTA'TAC

Cuts at:      0     287     534 
   Size:        287     247
 
EarI CTCTTCn'nnn_

Cuts at:      0      21     487     534 
   Size:         21     466      47

  Fragments arranged by size: 

                 466      47      21
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     338     534 
   Size:        256      82     196

  Fragments arranged by size: 

                 256     196      82
 
EcoO109I rG'GnC_Cy

Cuts at:      0      88     197     523     534 
   Size:         88     109     326      11

  Fragments arranged by size: 

                 326     109      88      11
 
NaeI GCC'GGC

Cuts at:      0       5     534 
   Size:          5     529
 
NgoMIV G'CCGG_C

Cuts at:      0       3     534 
   Size:          3     531
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     473     534 
   Size:        473      61
 
PfoI T'CCnGG_A

Cuts at:      0     315     534 
   Size:        315     219
 
PpuMI rG'GwC_Cy

Cuts at:      0      88     197     523     534 
   Size:         88     109     326      11

  Fragments arranged by size: 

                 326     109      88      11
 
StyI C'CwwG_G

Cuts at:      0     267     534 
   Size:        267     267
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     291     323     534 
   Size:        291      32     211

  Fragments arranged by size: 

                 291     211      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     442     534 
   Size:        442      92
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI    BamHI     BbsI     BpmI   Bpu10I    BseYI
    BspHI    BspMI    BsrFI   BstAPI    BstYI  BstZ17I     EarI  Eco57MI
 EcoO109I     NaeI   NgoMIV    PflMI     PfoI    PpuMI     StyI     TstI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI