Restriction map for
pF1KB0948
(Linear) (Six-base) MAPSORT of: KIBB0948.seq Check: 2408 from: 1 to: 1206
KIBB0948.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 0 216 1206
Size: 216 990
AflII C'TTAA_G
Cuts at: 0 900 1206
Size: 900 306
AflIII A'CryG_T
Cuts at: 0 211 1206
Size: 211 995
AlwNI CAG_nnn'CTG
Cuts at: 0 1046 1206
Size: 1046 160
BanI G'GyrC_C
Cuts at: 0 24 1206
Size: 24 1182
BbeI G_GCGC'C
Cuts at: 0 28 1206
Size: 28 1178
BbsI GAAGACnn'nnnn_
Cuts at: 0 82 1206
Size: 82 1124
BfrBI ATG'CAT
Cuts at: 0 1124 1206
Size: 1124 82
BmgBI CAC'GTC
Cuts at: 0 126 1206
Size: 126 1080
BmrI ACTGGGnnnn_n'
Cuts at: 0 443 1206
Size: 443 763
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 154 371 623 707 723 959 1040
Size: 97 57 217 252 84 16 236 81
Cuts at: 1040 1056 1124 1206
Size: 16 68 82
Fragments arranged by size:
252 236 217 97 84 82 81 68
57 16 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 777 1110 1206
Size: 777 333 96
Fragments arranged by size:
777 333 96
BsaI GGTCTCn'nnnn_
Cuts at: 0 130 1206
Size: 130 1076
BsaAI yAC'GTr
Cuts at: 0 212 1206
Size: 212 994
BsaHI Gr'CG_yC
Cuts at: 0 25 1206
Size: 25 1181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 474 726 1059 1206
Size: 390 84 252 333 147
Fragments arranged by size:
390 333 252 147 84
BsiHKAI G_wGCw'C
Cuts at: 0 211 1206
Size: 211 995
BsmI GAATG_Cn'
Cuts at: 0 625 793 1126 1206
Size: 625 168 333 80
Fragments arranged by size:
625 333 168 80
Bsp1286I G_dGCh'C
Cuts at: 0 211 1206
Size: 211 995
BspHI T'CATG_A
Cuts at: 0 417 1206
Size: 417 789
BsrBI CCG'CTC
Cuts at: 0 16 1206
Size: 16 1190
BsrDI GCAATG_nn'
Cuts at: 0 630 1206
Size: 630 576
BstYI r'GATC_y
Cuts at: 0 721 1206
Size: 721 485
BstZ17I GTA'TAC
Cuts at: 0 217 1206
Size: 217 989
BtsI GCAGTG_nn'
Cuts at: 0 45 462 714 967 1047 1206
Size: 45 417 252 253 80 159
Fragments arranged by size:
417 253 252 159 80 45
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 612 647 1206
Size: 612 35 559
Fragments arranged by size:
612 559 35
EarI CTCTTCn'nnn_
Cuts at: 0 315 492 828 1206
Size: 315 177 336 378
Fragments arranged by size:
378 336 315 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 154 371 623 707 723 959 1040
Size: 97 57 217 252 84 16 236 81
Cuts at: 1040 1056 1124 1206
Size: 16 68 82
Fragments arranged by size:
252 236 217 97 84 82 81 68
57 16 16
HaeII r_GCGC'y
Cuts at: 0 28 1206
Size: 28 1178
KasI G'GCGC_C
Cuts at: 0 24 1206
Size: 24 1182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 932 1206
Size: 932 274
MslI CAynn'nnrTG
Cuts at: 0 338 416 1119 1206
Size: 338 78 703 87
Fragments arranged by size:
703 338 87 78
NarI GG'CG_CC
Cuts at: 0 25 1206
Size: 25 1181
NdeI CA'TA_TG
Cuts at: 0 1116 1206
Size: 1116 90
NsiI A_TGCA'T
Cuts at: 0 1126 1206
Size: 1126 80
PasI CC'CwG_GG
Cuts at: 0 159 1206
Size: 159 1047
PmlI CAC'GTG
Cuts at: 0 212 1206
Size: 212 994
PsiI TTA'TAA
Cuts at: 0 1202 1206
Size: 1202 4
SapI GCTCTTCn'nnn_
Cuts at: 0 492 828 1206
Size: 492 336 378
Fragments arranged by size:
492 378 336
SfoI GGC'GCC
Cuts at: 0 26 1206
Size: 26 1180
SmlI C'TyrA_G
Cuts at: 0 756 900 1089 1206
Size: 756 144 189 117
Fragments arranged by size:
756 189 144 117
StuI AGG'CCT
Cuts at: 0 258 360 528 696 780 864 1029 1197
Size: 258 102 168 168 84 84 165 168
Cuts at: 1197 1206
Size: 9
Fragments arranged by size:
258 168 168 168 165 102 84 84
9
XmnI GAAnn'nnTTC
Cuts at: 0 822 1206
Size: 822 384
Enzymes that do cut:
AccI AflII AflIII AlwNI BanI BbeI BbsI BfrBI
BmgBI BmrI BpmI BpuEI BsaI BsaAI BsaHI BsgI
BsiHKAI BsmI Bsp1286I BspHI BsrBI BsrDI BstYI BstZ17I
BtsI CspCI EarI Eco57MI HaeII KasI MmeI MslI
NarI NdeI NsiI PasI PmlI PsiI SapI SfoI
SmlI StuI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmtI BplI
Bpu10I BsaBI BsaWI BsaXI BseRI BseYI BsiEI BsiWI
BsmBI BspEI BspMI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI Bsu36I BtgI BtgZI ClaI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MscI MspA1I NaeI
NcoI NgoMIV NheI NotI NruI NspI PacI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI