(Linear) (Six-base) MAPSORT of: KIBB0951.seq  Check: 4905  from: 1  to: 549



KIBB0951.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      73     105     549 
   Size:         73      32     444

  Fragments arranged by size: 

                 444      73      32
 
ApoI r'AATT_y

Cuts at:      0     513     549 
   Size:        513      36
 
AvaI C'yCGr_G

Cuts at:      0     329     449     549 
   Size:        329     120     100

  Fragments arranged by size: 

                 329     120     100
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      30      63     549 
   Size:         30      33     486

  Fragments arranged by size: 

                 486      33      30
 
BanI G'GyrC_C

Cuts at:      0     333     549 
   Size:        333     216
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      61     549 
   Size:         61     488
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     514     548     549 
   Size:        514      34       1

  Fragments arranged by size: 

                 514      34       1
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      31     549 
   Size:         31     518
 
BglII A'GATC_T

Cuts at:      0     363     549 
   Size:        363     186
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     356     549 
   Size:        356     193
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     452     549 
   Size:        452      97
 
BsmI GAATG_Cn'

Cuts at:      0     203     549 
   Size:        203     346
 
BstYI r'GATC_y

Cuts at:      0     363     549 
   Size:        363     186
 
BtgI C'CryG_G

Cuts at:      0     234     318     549 
   Size:        234      84     231

  Fragments arranged by size: 

                 234     231      84
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     356     549 
   Size:        356     193
 
EcoRI G'AATT_C

Cuts at:      0     513     549 
   Size:        513      36
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     126     158     549 
   Size:        126      32     391

  Fragments arranged by size: 

                 391     126      32
 
MslI CAynn'nnrTG

Cuts at:      0     239     549 
   Size:        239     310
 
MspA1I CmG'CkG

Cuts at:      0      44     462     549 
   Size:         44     418      87

  Fragments arranged by size: 

                 418      87      44
 
NcoI C'CATG_G

Cuts at:      0     234     318     549 
   Size:        234      84     231

  Fragments arranged by size: 

                 234     231      84
 
PfoI T'CCnGG_A

Cuts at:      0     491     549 
   Size:        491      58
 
PvuII CAG'CTG

Cuts at:      0     462     549 
   Size:        462      87
 
SfcI C'TryA_G

Cuts at:      0      15     110     549 
   Size:         15      95     439

  Fragments arranged by size: 

                 439      95      15
 
SmaI CCC'GGG

Cuts at:      0     331     549 
   Size:        331     218
 
SmlI C'TyrA_G

Cuts at:      0     449     467     549 
   Size:        449      18      82

  Fragments arranged by size: 

                 449      82      18
 
StyI C'CwwG_G

Cuts at:      0     234     318     549 
   Size:        234      84     231

  Fragments arranged by size: 

                 234     231      84
 
TatI w'GTAC_w

Cuts at:      0     197     549 
   Size:        197     352
 
XhoI C'TCGA_G

Cuts at:      0     449     549 
   Size:        449     100
 
XmaI C'CCGG_G

Cuts at:      0     329     549 
   Size:        329     220
 

 Enzymes that do cut:

     AloI     ApoI     AvaI     BaeI     BanI     BbsI     BcgI    BciVI
    BglII     BpmI    BpuEI     BsmI    BstYI     BtgI  Eco57MI    EcoRI
    Hin4I     MslI   MspA1I     NcoI     PfoI    PvuII     SfcI     SmaI
     SmlI     StyI     TatI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII    BamHI    BanII     BbeI    BbvCI
     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XmnI     ZraI