(Linear) (Six-base) MAPSORT of: KIBB0955.seq  Check: 9234  from: 1  to: 561



KIBB0955.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      64     561 
   Size:         64     497
 
ApaI G_GGCC'C

Cuts at:      0     147     236     561 
   Size:        147      89     325

  Fragments arranged by size: 

                 325     147      89
 
ApoI r'AATT_y

Cuts at:      0     301     561 
   Size:        301     260
 
AvaI C'yCGr_G

Cuts at:      0     154     561 
   Size:        154     407
 
BanI G'GyrC_C

Cuts at:      0     201     355     561 
   Size:        201     154     206

  Fragments arranged by size: 

                 206     201     154
 
BanII G_rGCy'C

Cuts at:      0     147     236     561 
   Size:        147      89     325

  Fragments arranged by size: 

                 325     147      89
 
BbeI G_GCGC'C

Cuts at:      0     205     561 
   Size:        205     356
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     463     561 
   Size:        463      98
 
BclI T'GATC_A

Cuts at:      0     468     561 
   Size:        468      93
 
BfrBI ATG'CAT

Cuts at:      0     447     561 
   Size:        447     114
 
Bme1580I G_kGCm'C

Cuts at:      0     147     236     561 
   Size:        147      89     325

  Fragments arranged by size: 

                 325     147      89
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     321     561 
   Size:        321     240
 
BsaHI Gr'CG_yC

Cuts at:      0     202     561 
   Size:        202     359
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     172     190     193     196     355     561 
   Size:        172      18       3       3     159     206

  Fragments arranged by size: 

                 206     172     159      18       3       3
 
BseYI C'CCAG_C

Cuts at:      0     188     241     561 
   Size:        188      53     320

  Fragments arranged by size: 

                 320     188      53
 
Bsp1286I G_dGCh'C

Cuts at:      0     147     236     561 
   Size:        147      89     325

  Fragments arranged by size: 

                 325     147      89
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      56     561 
   Size:         56     505
 
BsrBI CCG'CTC

Cuts at:      0     379     561 
   Size:        379     182
 
BsrFI r'CCGG_y

Cuts at:      0     358     561 
   Size:        358     203
 
BstEII G'GTnAC_C

Cuts at:      0      76     561 
   Size:         76     485
 
BtsI GCAGTG_nn'

Cuts at:      0      48     561 
   Size:         48     513
 
EarI CTCTTCn'nnn_

Cuts at:      0      29     332     482     561 
   Size:         29     303     150      79

  Fragments arranged by size: 

                 303     150      79      29
 
EcoO109I rG'GnC_Cy

Cuts at:      0     143     561 
   Size:        143     418
 
HaeII r_GCGC'y

Cuts at:      0     205     561 
   Size:        205     356
 
KasI G'GCGC_C

Cuts at:      0     201     561 
   Size:        201     360
 
MspA1I CmG'CkG

Cuts at:      0     104     140     561 
   Size:        104      36     421

  Fragments arranged by size: 

                 421     104      36
 
NaeI GCC'GGC

Cuts at:      0     360     561 
   Size:        360     201
 
NarI GG'CG_CC

Cuts at:      0     202     561 
   Size:        202     359
 
NgoMIV G'CCGG_C

Cuts at:      0     358     561 
   Size:        358     203
 
NsiI A_TGCA'T

Cuts at:      0     449     561 
   Size:        449     112
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     195     561 
   Size:        195     366
 
PspOMI G'GGCC_C

Cuts at:      0     143     232     561 
   Size:        143      89     329

  Fragments arranged by size: 

                 329     143      89
 
PspXI vC'TCGA_Gb

Cuts at:      0     154     561 
   Size:        154     407
 
PstI C_TGCA'G

Cuts at:      0      53     561 
   Size:         53     508
 
SapI GCTCTTCn'nnn_

Cuts at:      0     482     561 
   Size:        482      79
 
SfcI C'TryA_G

Cuts at:      0      49     363     561 
   Size:         49     314     198

  Fragments arranged by size: 

                 314     198      49
 
SfoI GGC'GCC

Cuts at:      0     203     561 
   Size:        203     358
 
SmlI C'TyrA_G

Cuts at:      0     154     561 
   Size:        154     407
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     538     561 
   Size:        538      23
 
XhoI C'TCGA_G

Cuts at:      0     154     561 
   Size:        154     407
 

 Enzymes that do cut:

     AlfI     ApaI     ApoI     AvaI     BanI    BanII     BbeI     BbsI
     BclI    BfrBI Bme1580I     BmrI    BsaHI    BseRI    BseYI Bsp1286I
    BspMI    BsrBI    BsrFI   BstEII     BtsI     EarI EcoO109I    HaeII
     KasI   MspA1I     NaeI     NarI   NgoMIV     NsiI    PflMI   PspOMI
    PspXI     PstI     SapI     SfcI     SfoI     SmlI    TaqII     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NcoI     NdeI     NheI
     NotI     NruI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI     ZraI