Restriction map for
pF1KB0959
(Linear) (Six-base) MAPSORT of: KIBB0959.seq Check: 1499 from: 1 to: 570
KIBB0959.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 240 300 432 465 558 570
Size: 240 60 132 33 93 12
Fragments arranged by size:
240 132 93 60 33 12
ApaLI G'TGCA_C
Cuts at: 0 346 570
Size: 346 224
BanI G'GyrC_C
Cuts at: 0 19 225 570
Size: 19 206 345
Fragments arranged by size:
345 206 19
BbeI G_GCGC'C
Cuts at: 0 229 570
Size: 229 341
BglI GCCn_nnn'nGGC
Cuts at: 0 284 423 570
Size: 284 139 147
Fragments arranged by size:
284 147 139
Bme1580I G_kGCm'C
Cuts at: 0 22 350 487 570
Size: 22 328 137 83
Fragments arranged by size:
328 137 83 22
BmgBI CAC'GTC
Cuts at: 0 59 570
Size: 59 511
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 570
Size: 487 83
BsaAI yAC'GTr
Cuts at: 0 447 570
Size: 447 123
BsaHI Gr'CG_yC
Cuts at: 0 226 514 570
Size: 226 288 56
Fragments arranged by size:
288 226 56
BsaWI w'CCGG_w
Cuts at: 0 335 570
Size: 335 235
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 160 190 570
Size: 160 30 380
Fragments arranged by size:
380 160 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 90 570
Size: 90 480
BseYI C'CCAG_C
Cuts at: 0 408 480 570
Size: 408 72 90
Fragments arranged by size:
408 90 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 570
Size: 253 317
BsiHKAI G_wGCw'C
Cuts at: 0 343 350 570
Size: 343 7 220
Fragments arranged by size:
343 220 7
BsmBI CGTCTCn'nnnn_
Cuts at: 0 505 570
Size: 505 65
Bsp1286I G_dGCh'C
Cuts at: 0 22 343 350 487 570
Size: 22 321 7 137 83
Fragments arranged by size:
321 137 83 22 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 308 570
Size: 308 262
BsrFI r'CCGG_y
Cuts at: 0 228 439 570
Size: 228 211 131
Fragments arranged by size:
228 211 131
BssHII G'CGCG_C
Cuts at: 0 328 570
Size: 328 242
BssSI C'ACGA_G
Cuts at: 0 38 570
Size: 38 532
BstAPI GCAn_nnn'nTGC
Cuts at: 0 432 570
Size: 432 138
BstEII G'GTnAC_C
Cuts at: 0 354 570
Size: 354 216
Bsu36I CC'TnA_GG
Cuts at: 0 262 570
Size: 262 308
DraIII CAC_nnn'GTG
Cuts at: 0 354 570
Size: 354 216
EaeI y'GGCC_r
Cuts at: 0 441 570
Size: 441 129
EarI CTCTTCn'nnn_
Cuts at: 0 88 570
Size: 88 482
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 570
Size: 487 83
EcoO109I rG'GnC_Cy
Cuts at: 0 26 47 208 237 267 570
Size: 26 21 161 29 30 303
Fragments arranged by size:
303 161 30 29 26 21
FspI TGC'GCA
Cuts at: 0 304 451 570
Size: 304 147 119
Fragments arranged by size:
304 147 119
HaeII r_GCGC'y
Cuts at: 0 229 570
Size: 229 341
KasI G'GCGC_C
Cuts at: 0 225 570
Size: 225 345
MspA1I CmG'CkG
Cuts at: 0 308 432 558 570
Size: 308 124 126 12
Fragments arranged by size:
308 126 124 12
NaeI GCC'GGC
Cuts at: 0 441 570
Size: 441 129
NarI GG'CG_CC
Cuts at: 0 226 570
Size: 226 344
NgoMIV G'CCGG_C
Cuts at: 0 439 570
Size: 439 131
NspI r_CATG'y
Cuts at: 0 278 570
Size: 278 292
PmlI CAC'GTG
Cuts at: 0 447 570
Size: 447 123
PpuMI rG'GwC_Cy
Cuts at: 0 26 267 570
Size: 26 241 303
Fragments arranged by size:
303 241 26
PstI C_TGCA'G
Cuts at: 0 461 503 570
Size: 461 42 67
Fragments arranged by size:
461 67 42
PvuII CAG'CTG
Cuts at: 0 558 570
Size: 558 12
SanDI GG'GwC_CC
Cuts at: 0 267 570
Size: 267 303
SbfI CC_TGCA'GG
Cuts at: 0 461 570
Size: 461 109
SfcI C'TryA_G
Cuts at: 0 457 499 570
Size: 457 42 71
Fragments arranged by size:
457 71 42
SfoI GGC'GCC
Cuts at: 0 227 570
Size: 227 343
SgrAI Cr'CCGG_yG
Cuts at: 0 228 570
Size: 228 342
StuI AGG'CCT
Cuts at: 0 364 570
Size: 364 206
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 43 75 570
Size: 43 32 495
Fragments arranged by size:
495 43 32
Enzymes that do cut:
AlwNI ApaLI BanI BbeI BglI Bme1580I BmgBI BpmI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspMI BsrFI BssHII BssSI BstAPI BstEII
Bsu36I DraIII EaeI EarI Eco57MI EcoO109I FspI HaeII
KasI MspA1I NaeI NarI NgoMIV NspI PmlI PpuMI
PstI PvuII SanDI SbfI SfcI SfoI SgrAI StuI
TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsiEI
BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BstBI
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI ScaI SexAI SfiI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StyI SwaI TaqII TatI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI