(Linear) (Six-base) MAPSORT of: KIBB0961.seq  Check: 7310  from: 1  to: 579



KIBB0961.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AfeI AGC'GCT

Cuts at:      0     352     579 
   Size:        352     227
 
ApaI G_GGCC'C

Cuts at:      0     203     530     579 
   Size:        203     327      49

  Fragments arranged by size: 

                 327     203      49
 
ApoI r'AATT_y

Cuts at:      0     322     579 
   Size:        322     257
 
BanI G'GyrC_C

Cuts at:      0     232     579 
   Size:        232     347
 
BanII G_rGCy'C

Cuts at:      0     203     530     579 
   Size:        203     327      49

  Fragments arranged by size: 

                 327     203      49
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      49     579 
   Size:         49     530
 
BbvCI CC'TCA_GC

Cuts at:      0     238     579 
   Size:        238     341
 
BfrBI ATG'CAT

Cuts at:      0     316     579 
   Size:        316     263
 
Bme1580I G_kGCm'C

Cuts at:      0     203     530     579 
   Size:        203     327      49

  Fragments arranged by size: 

                 327     203      49
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      18     579 
   Size:         18     561
 
Bpu10I CC'TnA_GC

Cuts at:      0     238     579 
   Size:        238     341
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     478     579 
   Size:        478     101
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      29     175     579 
   Size:         29     146     404

  Fragments arranged by size: 

                 404     146      29
 
BseYI C'CCAG_C

Cuts at:      0     261     403     556     579 
   Size:        261     142     153      23

  Fragments arranged by size: 

                 261     153     142      23
 
Bsp1286I G_dGCh'C

Cuts at:      0     203     530     579 
   Size:        203     327      49

  Fragments arranged by size: 

                 327     203      49
 
BssSI C'ACGA_G

Cuts at:      0     274     579 
   Size:        274     305
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     116     179     379     579 
   Size:        116      63     200     200

  Fragments arranged by size: 

                 200     200     116      63
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     337     579 
   Size:        337     242
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0       9      44     579 
   Size:          9      35     535

  Fragments arranged by size: 

                 535      35       9
 
EaeI y'GGCC_r

Cuts at:      0     146     288     579 
   Size:        146     142     291

  Fragments arranged by size: 

                 291     146     142
 
EarI CTCTTCn'nnn_

Cuts at:      0      56     579 
   Size:         56     523
 
EcoO109I rG'GnC_Cy

Cuts at:      0     560     579 
   Size:        560      19
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     444     579 
   Size:        444     135
 
HaeII r_GCGC'y

Cuts at:      0     354     579 
   Size:        354     225
 
HincII GTy'rAC

Cuts at:      0     464     579 
   Size:        464     115
 
MscI TGG'CCA

Cuts at:      0     290     579 
   Size:        290     289
 
NsiI A_TGCA'T

Cuts at:      0     318     579 
   Size:        318     261
 
PspOMI G'GGCC_C

Cuts at:      0     199     526     579 
   Size:        199     327      53

  Fragments arranged by size: 

                 327     199      53
 
SmlI C'TyrA_G

Cuts at:      0     457     579 
   Size:        457     122
 
StuI AGG'CCT

Cuts at:      0     424     455     579 
   Size:        424      31     124

  Fragments arranged by size: 

                 424     124      31
 
StyI C'CwwG_G

Cuts at:      0     530     563     579 
   Size:        530      33      16

  Fragments arranged by size: 

                 530      33      16
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     337     579 
   Size:        337     242
 
XmnI GAAnn'nnTTC

Cuts at:      0      65     579 
   Size:         65     514
 

 Enzymes that do cut:

     AfeI     ApaI     ApoI     BanI    BanII     BbsI    BbvCI    BfrBI
 Bme1580I     BmrI   Bpu10I    BpuEI    BseRI    BseYI Bsp1286I    BssSI
   BstAPI    BstXI    CspCI     EaeI     EarI EcoO109I     FalI    HaeII
   HincII     MscI     NsiI   PspOMI     SmlI     StuI     StyI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BcgI
    BciVI     BclI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
     BpmI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    Hin4I  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     ZraI