Restriction map for
pF1KB0962
(Linear) (Six-base) MAPSORT of: KIBB0962.seq Check: 6230 from: 1 to: 1275
KIBB0962.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 1046 1275
Size: 724 322 229
Fragments arranged by size:
724 322 229
AlwNI CAG_nnn'CTG
Cuts at: 0 738 1275
Size: 738 537
ApoI r'AATT_y
Cuts at: 0 550 588 874 1275
Size: 550 38 286 401
Fragments arranged by size:
550 401 286 38
AvaI C'yCGr_G
Cuts at: 0 285 1006 1275
Size: 285 721 269
Fragments arranged by size:
721 285 269
BamHI G'GATC_C
Cuts at: 0 539 1275
Size: 539 736
BanI G'GyrC_C
Cuts at: 0 290 1275
Size: 290 985
BanII G_rGCy'C
Cuts at: 0 1007 1168 1211 1275
Size: 1007 161 43 64
Fragments arranged by size:
1007 161 64 43
BbsI GAAGACnn'nnnn_
Cuts at: 0 498 712 1186 1275
Size: 498 214 474 89
Fragments arranged by size:
498 474 214 89
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 382 416 1275
Size: 382 34 859
Fragments arranged by size:
859 382 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1167 1275
Size: 1167 108
BfrBI ATG'CAT
Cuts at: 0 116 1275
Size: 116 1159
BglI GCCn_nnn'nGGC
Cuts at: 0 729 1275
Size: 729 546
Bme1580I G_kGCm'C
Cuts at: 0 98 1275
Size: 98 1177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 835 874 1275
Size: 760 75 39 401
Fragments arranged by size:
760 401 75 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 1275
Size: 667 608
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 592 595 1219 1275
Size: 592 3 624 56
Fragments arranged by size:
624 592 56 3
BseYI C'CCAG_C
Cuts at: 0 615 956 1099 1275
Size: 615 341 143 176
Fragments arranged by size:
615 341 176 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 783 1275
Size: 277 506 492
Fragments arranged by size:
506 492 277
BsiHKAI G_wGCw'C
Cuts at: 0 1168 1211 1275
Size: 1168 43 64
Fragments arranged by size:
1168 64 43
Bsp1286I G_dGCh'C
Cuts at: 0 98 1007 1168 1211 1275
Size: 98 909 161 43 64
Fragments arranged by size:
909 161 98 64 43
BspHI T'CATG_A
Cuts at: 0 621 1275
Size: 621 654
BsrGI T'GTAC_A
Cuts at: 0 489 1275
Size: 489 786
BstAPI GCAn_nnn'nTGC
Cuts at: 0 720 1275
Size: 720 555
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 1275
Size: 57 1218
BstYI r'GATC_y
Cuts at: 0 434 539 1275
Size: 434 105 736
Fragments arranged by size:
736 434 105
DraIII CAC_nnn'GTG
Cuts at: 0 487 1225 1275
Size: 487 738 50
Fragments arranged by size:
738 487 50
EaeI y'GGCC_r
Cuts at: 0 2 1275
Size: 2 1273
EarI CTCTTCn'nnn_
Cuts at: 0 767 946 1065 1275
Size: 767 179 119 210
Fragments arranged by size:
767 210 179 119
EcoICRI GAG'CTC
Cuts at: 0 1166 1209 1275
Size: 1166 43 66
Fragments arranged by size:
1166 66 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 760 835 874 1046 1275
Size: 724 36 75 39 172 229
Fragments arranged by size:
724 229 172 75 39 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 577 1275
Size: 577 698
EcoRV GAT'ATC
Cuts at: 0 747 1275
Size: 747 528
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 244 276 355 387 994 1026 1137 1169
Size: 244 32 79 32 607 32 111 32
Cuts at: 1169 1275
Size: 106
Fragments arranged by size:
607 244 111 106 79 32 32 32
32
MscI TGG'CCA
Cuts at: 0 4 1275
Size: 4 1271
MspA1I CmG'CkG
Cuts at: 0 735 1275
Size: 735 540
NsiI A_TGCA'T
Cuts at: 0 118 1275
Size: 118 1157
NspI r_CATG'y
Cuts at: 0 284 723 1275
Size: 284 439 552
Fragments arranged by size:
552 439 284
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 982 1014 1275
Size: 982 32 261
Fragments arranged by size:
982 261 32
PstI C_TGCA'G
Cuts at: 0 71 1023 1275
Size: 71 952 252
Fragments arranged by size:
952 252 71
PvuII CAG'CTG
Cuts at: 0 735 1275
Size: 735 540
SacI G_AGCT'C
Cuts at: 0 1168 1211 1275
Size: 1168 43 64
Fragments arranged by size:
1168 64 43
SapI GCTCTTCn'nnn_
Cuts at: 0 946 1275
Size: 946 329
SexAI A'CCwGG_T
Cuts at: 0 515 1275
Size: 515 760
SfcI C'TryA_G
Cuts at: 0 67 1019 1275
Size: 67 952 256
Fragments arranged by size:
952 256 67
SmaI CCC'GGG
Cuts at: 0 1008 1275
Size: 1008 267
SmlI C'TyrA_G
Cuts at: 0 682 1275
Size: 682 593
StyI C'CwwG_G
Cuts at: 0 359 1275
Size: 359 916
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 741 1275
Size: 741 534
TatI w'GTAC_w
Cuts at: 0 468 489 1275
Size: 468 21 786
Fragments arranged by size:
786 468 21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 735 1275
Size: 735 540
XmaI C'CCGG_G
Cuts at: 0 1006 1275
Size: 1006 269
XmnI GAAnn'nnTTC
Cuts at: 0 589 776 874 1275
Size: 589 187 98 401
Fragments arranged by size:
589 401 187 98
Enzymes that do cut:
AcuI AlwNI ApoI AvaI BamHI BanI BanII BbsI
BcgI BciVI BfrBI BglI Bme1580I BpmI BpuEI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspHI BsrGI BstAPI BstXI
BstYI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoRV
Hin4I MscI MspA1I NsiI NspI PpiI PstI PvuII
SacI SapI SexAI SfcI SmaI SmlI StyI TaqII
TatI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BbeI BbvCI BclI BglII
BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EagI EciI EcoO109I EcoRI FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PflMI
PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XhoI ZraI