(Linear) (Six-base) MAPSORT of: KIBB0963.seq  Check: 7485  from: 1  to: 579



KIBB0963.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AleI CACnn'nnGTG

Cuts at:      0     142     579 
   Size:        142     437
 
ApaI G_GGCC'C

Cuts at:      0     530     579 
   Size:        530      49
 
ApoI r'AATT_y

Cuts at:      0     126     322     579 
   Size:        126     196     257

  Fragments arranged by size: 

                 257     196     126
 
BanII G_rGCy'C

Cuts at:      0     530     564     579 
   Size:        530      34      15

  Fragments arranged by size: 

                 530      34      15
 
BglI GCCn_nnn'nGGC

Cuts at:      0     294     579 
   Size:        294     285
 
BglII A'GATC_T

Cuts at:      0     164     579 
   Size:        164     415
 
Bme1580I G_kGCm'C

Cuts at:      0     530     579 
   Size:        530      49
 
BseYI C'CCAG_C

Cuts at:      0     261     579 
   Size:        261     318
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     579 
   Size:         88     491
 
Bsp1286I G_dGCh'C

Cuts at:      0     530     564     579 
   Size:        530      34      15

  Fragments arranged by size: 

                 530      34      15
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     116     179     579 
   Size:        116      63     400

  Fragments arranged by size: 

                 400     116      63
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     229     579 
   Size:        229     350
 
BstYI r'GATC_y

Cuts at:      0     164     579 
   Size:        164     415
 
BtgI C'CryG_G

Cuts at:      0     249     579 
   Size:        249     330
 
BtsI GCAGTG_nn'

Cuts at:      0      76     579 
   Size:         76     503
 
EaeI y'GGCC_r

Cuts at:      0     219     546     579 
   Size:        219     327      33

  Fragments arranged by size: 

                 327     219      33
 
EarI CTCTTCn'nnn_

Cuts at:      0      56     155     445     579 
   Size:         56      99     290     134

  Fragments arranged by size: 

                 290     134      99      56
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      39     579 
   Size:         39     540
 
EcoO109I rG'GnC_Cy

Cuts at:      0      32     579 
   Size:         32     547
 
HaeII r_GCGC'y

Cuts at:      0     230     579 
   Size:        230     349
 
MscI TGG'CCA

Cuts at:      0     221     548     579 
   Size:        221     327      31

  Fragments arranged by size: 

                 327     221      31
 
MslI CAynn'nnrTG

Cuts at:      0     142     579 
   Size:        142     437
 
MspA1I CmG'CkG

Cuts at:      0      95     579 
   Size:         95     484
 
PspOMI G'GGCC_C

Cuts at:      0     526     579 
   Size:        526      53
 
StuI AGG'CCT

Cuts at:      0     297     579 
   Size:        297     282
 
StyI C'CwwG_G

Cuts at:      0     530     549     579 
   Size:        530      19      30

  Fragments arranged by size: 

                 530      30      19
 

 Enzymes that do cut:

     AleI     ApaI     ApoI    BanII     BglI    BglII Bme1580I    BseYI
     BsgI Bsp1286I   BstAPI    BstXI    BstYI     BtgI     BtsI     EaeI
     EarI    EcoNI EcoO109I    HaeII     MscI     MslI   MspA1I   PspOMI
     StuI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
  Eco57MI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI