(Linear) (Six-base) MAPSORT of: KIBB0965.seq  Check: 6646  from: 1  to: 582



KIBB0965.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AccI GT'mk_AC

Cuts at:      0      50     582 
   Size:         50     532
 
AflIII A'CryG_T

Cuts at:      0      97     582 
   Size:         97     485
 
ApoI r'AATT_y

Cuts at:      0     325     522     582 
   Size:        325     197      60

  Fragments arranged by size: 

                 325     197      60
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     126     160     582 
   Size:        126      34     422

  Fragments arranged by size: 

                 422     126      34
 
BglII A'GATC_T

Cuts at:      0     164     582 
   Size:        164     418
 
Bme1580I G_kGCm'C

Cuts at:      0     361     582 
   Size:        361     221
 
BmgBI CAC'GTC

Cuts at:      0      98     582 
   Size:         98     484
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      18     582 
   Size:         18     564
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     481     582 
   Size:        481     101
 
Bsp1286I G_dGCh'C

Cuts at:      0     361     582 
   Size:        361     221
 
BspHI T'CATG_A

Cuts at:      0     153     582 
   Size:        153     429
 
BsrDI GCAATG_nn'

Cuts at:      0      81     582 
   Size:         81     501
 
BsrFI r'CCGG_y

Cuts at:      0     291     582 
   Size:        291     291
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     116     179     509     582 
   Size:        116      63     330      73

  Fragments arranged by size: 

                 330     116      73      63
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      76     582 
   Size:         76     506
 
BstYI r'GATC_y

Cuts at:      0     164     582 
   Size:        164     418
 
BtgI C'CryG_G

Cuts at:      0     533     582 
   Size:        533      49
 
BtsI GCAGTG_nn'

Cuts at:      0     105     329     582 
   Size:        105     224     253

  Fragments arranged by size: 

                 253     224     105
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     522     557     582 
   Size:        522      35      25

  Fragments arranged by size: 

                 522      35      25
 
EarI CTCTTCn'nnn_

Cuts at:      0     448     582 
   Size:        448     134
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      29     582 
   Size:         29     553
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     481     582 
   Size:        481     101
 
HaeII r_GCGC'y

Cuts at:      0     230     582 
   Size:        230     352
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     300     582 
   Size:        140     160     282

  Fragments arranged by size: 

                 282     160     140
 
MspA1I CmG'CkG

Cuts at:      0     437     582 
   Size:        437     145
 
NaeI GCC'GGC

Cuts at:      0     293     582 
   Size:        293     289
 
NgoMIV G'CCGG_C

Cuts at:      0     291     582 
   Size:        291     291
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     111     582 
   Size:        111     471
 
PstI C_TGCA'G

Cuts at:      0     121     272     582 
   Size:        121     151     310

  Fragments arranged by size: 

                 310     151     121
 
SbfI CC_TGCA'GG

Cuts at:      0     121     582 
   Size:        121     461
 
SfcI C'TryA_G

Cuts at:      0     117     268     582 
   Size:        117     151     314

  Fragments arranged by size: 

                 314     151     117
 
StuI AGG'CCT

Cuts at:      0     554     582 
   Size:        554      28
 
StyI C'CwwG_G

Cuts at:      0     415     582 
   Size:        415     167
 

 Enzymes that do cut:

     AccI   AflIII     ApoI     BcgI    BglII Bme1580I    BmgBI     BmrI
     BpmI Bsp1286I    BspHI    BsrDI    BsrFI   BstAPI    BstXI    BstYI
     BtgI     BtsI    CspCI     EarI     EciI  Eco57MI    HaeII     MmeI
   MspA1I     NaeI   NgoMIV    PflMI     PstI     SbfI     SfcI     StuI
     StyI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI     BlpI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrGI   BssHII    BssSI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI     DraI   DraIII
     DrdI     EaeI     EagI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI