Restriction map for
pF1KB0967
(Linear) (Six-base) MAPSORT of: KIBB0967.seq Check: 196 from: 1 to: 618
KIBB0967.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 563 618
Size: 563 55
AvaI C'yCGr_G
Cuts at: 0 25 60 278 618
Size: 25 35 218 340
Fragments arranged by size:
340 218 35 25
AvrII C'CTAG_G
Cuts at: 0 207 618
Size: 207 411
BanI G'GyrC_C
Cuts at: 0 161 211 236 618
Size: 161 50 25 382
Fragments arranged by size:
382 161 50 25
BanII G_rGCy'C
Cuts at: 0 26 61 91 618
Size: 26 35 30 527
Fragments arranged by size:
527 35 30 26
BbeI G_GCGC'C
Cuts at: 0 165 240 618
Size: 165 75 378
Fragments arranged by size:
378 165 75
BbvCI CC'TCA_GC
Cuts at: 0 169 440 518 618
Size: 169 271 78 100
Fragments arranged by size:
271 169 100 78
BlpI GC'TnA_GC
Cuts at: 0 284 618
Size: 284 334
BmgBI CAC'GTC
Cuts at: 0 351 618
Size: 351 267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 618
Size: 75 543
Bpu10I CC'TnA_GC
Cuts at: 0 169 440 518 618
Size: 169 271 78 100
Fragments arranged by size:
271 169 100 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 618
Size: 331 287
BsaHI Gr'CG_yC
Cuts at: 0 162 237 409 618
Size: 162 75 172 209
Fragments arranged by size:
209 172 162 75
BseYI C'CCAG_C
Cuts at: 0 288 586 618
Size: 288 298 32
Fragments arranged by size:
298 288 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 618
Size: 523 95
Bsp1286I G_dGCh'C
Cuts at: 0 26 61 91 618
Size: 26 35 30 527
Fragments arranged by size:
527 35 30 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 414 545 618
Size: 414 131 73
Fragments arranged by size:
414 131 73
BsrDI GCAATG_nn'
Cuts at: 0 403 618
Size: 403 215
BstXI CCAn_nnnn'nTGG
Cuts at: 0 266 618
Size: 266 352
Bsu36I CC'TnA_GG
Cuts at: 0 18 618
Size: 18 600
BtsI GCAGTG_nn'
Cuts at: 0 519 618
Size: 519 99
EaeI y'GGCC_r
Cuts at: 0 137 618
Size: 137 481
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 618
Size: 75 543
EcoNI CCTnn'n_nnAGG
Cuts at: 0 383 618
Size: 383 235
EcoO109I rG'GnC_Cy
Cuts at: 0 50 426 522 597 618
Size: 50 376 96 75 21
Fragments arranged by size:
376 96 75 50 21
HaeII r_GCGC'y
Cuts at: 0 165 240 578 618
Size: 165 75 338 40
Fragments arranged by size:
338 165 75 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 277 309 618
Size: 277 32 309
Fragments arranged by size:
309 277 32
KasI G'GCGC_C
Cuts at: 0 161 236 618
Size: 161 75 382
Fragments arranged by size:
382 161 75
MscI TGG'CCA
Cuts at: 0 139 618
Size: 139 479
MspA1I CmG'CkG
Cuts at: 0 586 618
Size: 586 32
NarI GG'CG_CC
Cuts at: 0 162 237 618
Size: 162 75 381
Fragments arranged by size:
381 162 75
PasI CC'CwG_GG
Cuts at: 0 379 618
Size: 379 239
PpuMI rG'GwC_Cy
Cuts at: 0 426 618
Size: 426 192
PstI C_TGCA'G
Cuts at: 0 275 371 618
Size: 275 96 247
Fragments arranged by size:
275 247 96
PvuII CAG'CTG
Cuts at: 0 586 618
Size: 586 32
SbfI CC_TGCA'GG
Cuts at: 0 371 618
Size: 371 247
SexAI A'CCwGG_T
Cuts at: 0 473 618
Size: 473 145
SfcI C'TryA_G
Cuts at: 0 271 367 618
Size: 271 96 251
Fragments arranged by size:
271 251 96
SfoI GGC'GCC
Cuts at: 0 163 238 618
Size: 163 75 380
Fragments arranged by size:
380 163 75
SmlI C'TyrA_G
Cuts at: 0 310 618
Size: 310 308
StyI C'CwwG_G
Cuts at: 0 53 207 618
Size: 53 154 411
Fragments arranged by size:
411 154 53
Tth111I GACn'n_nGTC
Cuts at: 0 349 475 618
Size: 349 126 143
Fragments arranged by size:
349 143 126
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 355 618
Size: 355 263
Enzymes that do cut:
AlfI AvaI AvrII BanI BanII BbeI BbvCI BlpI
BmgBI BpmI Bpu10I BpuEI BsaHI BseYI BsgI Bsp1286I
BspMI BsrDI BstXI Bsu36I BtsI EaeI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI MscI MspA1I NarI PasI
PpuMI PstI PvuII SbfI SexAI SfcI SfoI SmlI
StyI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI BaeI BamHI BbsI BcgI
BciVI BclI BfrBI BglI BglII Bme1580I BmrI BmtI
BplI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI
EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI ScaI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI TstI XbaI XhoI XmaI XmnI
ZraI